Diffusional restrictions in glyoxyl-agarose immobilized penicillin G acylase of different particle size and protein loading

Particle size and enzyme protein loading are design parameters of enzyme immobilization affecting biocatalyst performance that can be varied within broad margins. Their effect on mass transfer limitations at different bulk penicillin G concentrations has been studied with glyoxyl agarose immobilized...

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Autores principales:	Illanes,Andrés, González,José Miguel, Gómez,José Manuel, Valencia,Pedro, Wilson,Lorena
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2010
Materias:	diffusional restrictions enzyme kinetics immobilized enzyme penicillin G acylase penicillin hydrolysis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100002
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spelling	oai:scielo:S0717-345820100001000022010-04-28Diffusional restrictions in glyoxyl-agarose immobilized penicillin G acylase of different particle size and protein loadingIllanes,AndrésGonzález,José MiguelGómez,José ManuelValencia,PedroWilson,Lorena diffusional restrictions enzyme kinetics immobilized enzyme penicillin G acylase penicillin hydrolysis Particle size and enzyme protein loading are design parameters of enzyme immobilization affecting biocatalyst performance that can be varied within broad margins. Their effect on mass transfer limitations at different bulk penicillin G concentrations has been studied with glyoxyl agarose immobilized penicillin G acylase biocatalysts of average particle size of 5·10-5m and 10·10-4m at protein loadings from 15 to 130 mg/g gel. Internal diffusional restrictions were evaluated for such biocatalysts: Thiele modulus varied from 1.17 for the small particles at the lower protein load to 5.84 for the large particles at the higher protein load. Effectiveness factors at different bulk substrate concentrations were determined for all biocatalysts, values ranging from 0.78 for small particle size at 25 mM penicillin G to 0.15 for large particle size at 2 mM penicillin G. Enzyme protein loading had a strong impact on the effectiveness factors of immobilized penicillin G acylase, being it more pronounced in the case of large particle size biocatalysts. At conditions in which 6-aminopenicillanic acid is industrially produced, all biocatalysts tested were mass-transfer limited, being this information valuable for reactor design and performance evaluation.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.13 n.1 20102010-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100002en10.4067/S0717-34582010000100002
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	diffusional restrictions enzyme kinetics immobilized enzyme penicillin G acylase penicillin hydrolysis
spellingShingle	diffusional restrictions enzyme kinetics immobilized enzyme penicillin G acylase penicillin hydrolysis Illanes,Andrés González,José Miguel Gómez,José Manuel Valencia,Pedro Wilson,Lorena Diffusional restrictions in glyoxyl-agarose immobilized penicillin G acylase of different particle size and protein loading
description	Particle size and enzyme protein loading are design parameters of enzyme immobilization affecting biocatalyst performance that can be varied within broad margins. Their effect on mass transfer limitations at different bulk penicillin G concentrations has been studied with glyoxyl agarose immobilized penicillin G acylase biocatalysts of average particle size of 5·10-5m and 10·10-4m at protein loadings from 15 to 130 mg/g gel. Internal diffusional restrictions were evaluated for such biocatalysts: Thiele modulus varied from 1.17 for the small particles at the lower protein load to 5.84 for the large particles at the higher protein load. Effectiveness factors at different bulk substrate concentrations were determined for all biocatalysts, values ranging from 0.78 for small particle size at 25 mM penicillin G to 0.15 for large particle size at 2 mM penicillin G. Enzyme protein loading had a strong impact on the effectiveness factors of immobilized penicillin G acylase, being it more pronounced in the case of large particle size biocatalysts. At conditions in which 6-aminopenicillanic acid is industrially produced, all biocatalysts tested were mass-transfer limited, being this information valuable for reactor design and performance evaluation.
author	Illanes,Andrés González,José Miguel Gómez,José Manuel Valencia,Pedro Wilson,Lorena
author_facet	Illanes,Andrés González,José Miguel Gómez,José Manuel Valencia,Pedro Wilson,Lorena
author_sort	Illanes,Andrés
title	Diffusional restrictions in glyoxyl-agarose immobilized penicillin G acylase of different particle size and protein loading
title_short	Diffusional restrictions in glyoxyl-agarose immobilized penicillin G acylase of different particle size and protein loading
title_full	Diffusional restrictions in glyoxyl-agarose immobilized penicillin G acylase of different particle size and protein loading
title_fullStr	Diffusional restrictions in glyoxyl-agarose immobilized penicillin G acylase of different particle size and protein loading
title_full_unstemmed	Diffusional restrictions in glyoxyl-agarose immobilized penicillin G acylase of different particle size and protein loading
title_sort	diffusional restrictions in glyoxyl-agarose immobilized penicillin g acylase of different particle size and protein loading
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100002
work_keys_str_mv	AT illanesandres diffusionalrestrictionsinglyoxylagaroseimmobilizedpenicillingacylaseofdifferentparticlesizeandproteinloading AT gonzalezjosemiguel diffusionalrestrictionsinglyoxylagaroseimmobilizedpenicillingacylaseofdifferentparticlesizeandproteinloading AT gomezjosemanuel diffusionalrestrictionsinglyoxylagaroseimmobilizedpenicillingacylaseofdifferentparticlesizeandproteinloading AT valenciapedro diffusionalrestrictionsinglyoxylagaroseimmobilizedpenicillingacylaseofdifferentparticlesizeandproteinloading AT wilsonlorena diffusionalrestrictionsinglyoxylagaroseimmobilizedpenicillingacylaseofdifferentparticlesizeandproteinloading
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Diffusional restrictions in glyoxyl-agarose immobilized penicillin G acylase of different particle size and protein loading

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