Enhancement of wanlongmycin production by nitrogen ion beam implantation

In an attempt to obtain an industrial strain with higher yield of wanlongmycin, the wild strain Streptomyces griseovariabilis GAAS2507 was mutated by a novel mutagen, nitrogen ion beam with energy of 20 kilo electron volts (KeV) and dose ranging from 7.80 x 10(14) to 2.86 x 10(15) ions/cm². One muta...

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Autores principales:	Zhang,Xiaoyong, Lin,Birun, Gao,Xiangyang, Shen,Huifang
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2010
Materias:	mutagen nitrogen ion beam implantation Streptomyces griseovariabilis GAAS2057 wanlongmycin
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100003
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spelling	oai:scielo:S0717-345820100001000032010-04-28Enhancement of wanlongmycin production by nitrogen ion beam implantationZhang,XiaoyongLin,BirunGao,XiangyangShen,Huifang mutagen nitrogen ion beam implantation Streptomyces griseovariabilis GAAS2057 wanlongmycin In an attempt to obtain an industrial strain with higher yield of wanlongmycin, the wild strain Streptomyces griseovariabilis GAAS2507 was mutated by a novel mutagen, nitrogen ion beam with energy of 20 kilo electron volts (KeV) and dose ranging from 7.80 x 10(14) to 2.86 x 10(15) ions/cm². One mutant strain WN939 was obtained. Its yield of wanlongmycin reached 271.24 µg/mL, which was 82.10% higher than that of the wild strain. The mutant strain WN939 was relatively stable for the production of wanlongmycin through six successive transfers of cultures and a repeat fermentation in a 30 L fermentor. In addition, the mutant strains were investigated and divided into five types by their colony phenotypes and production of wanlongmycin. Among them, three types mutant strains exhibited positive mutation, while the other two types mutant strains exhibited negative mutation.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.13 n.1 20102010-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100003en10.4067/S0717-34582010000100003
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	mutagen nitrogen ion beam implantation Streptomyces griseovariabilis GAAS2057 wanlongmycin
spellingShingle	mutagen nitrogen ion beam implantation Streptomyces griseovariabilis GAAS2057 wanlongmycin Zhang,Xiaoyong Lin,Birun Gao,Xiangyang Shen,Huifang Enhancement of wanlongmycin production by nitrogen ion beam implantation
description	In an attempt to obtain an industrial strain with higher yield of wanlongmycin, the wild strain Streptomyces griseovariabilis GAAS2507 was mutated by a novel mutagen, nitrogen ion beam with energy of 20 kilo electron volts (KeV) and dose ranging from 7.80 x 10(14) to 2.86 x 10(15) ions/cm². One mutant strain WN939 was obtained. Its yield of wanlongmycin reached 271.24 µg/mL, which was 82.10% higher than that of the wild strain. The mutant strain WN939 was relatively stable for the production of wanlongmycin through six successive transfers of cultures and a repeat fermentation in a 30 L fermentor. In addition, the mutant strains were investigated and divided into five types by their colony phenotypes and production of wanlongmycin. Among them, three types mutant strains exhibited positive mutation, while the other two types mutant strains exhibited negative mutation.
author	Zhang,Xiaoyong Lin,Birun Gao,Xiangyang Shen,Huifang
author_facet	Zhang,Xiaoyong Lin,Birun Gao,Xiangyang Shen,Huifang
author_sort	Zhang,Xiaoyong
title	Enhancement of wanlongmycin production by nitrogen ion beam implantation
title_short	Enhancement of wanlongmycin production by nitrogen ion beam implantation
title_full	Enhancement of wanlongmycin production by nitrogen ion beam implantation
title_fullStr	Enhancement of wanlongmycin production by nitrogen ion beam implantation
title_full_unstemmed	Enhancement of wanlongmycin production by nitrogen ion beam implantation
title_sort	enhancement of wanlongmycin production by nitrogen ion beam implantation
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100003
work_keys_str_mv	AT zhangxiaoyong enhancementofwanlongmycinproductionbynitrogenionbeamimplantation AT linbirun enhancementofwanlongmycinproductionbynitrogenionbeamimplantation AT gaoxiangyang enhancementofwanlongmycinproductionbynitrogenionbeamimplantation AT shenhuifang enhancementofwanlongmycinproductionbynitrogenionbeamimplantation
_version_	1718441807193309184

Enhancement of wanlongmycin production by nitrogen ion beam implantation

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