In vitro regeneration and genetic fidelity of Tigridia pavonia (L.f.) DC.

Plants of Tigridia pavonia (L.f.) DC were regenerated from twin-scaling explants cultured on Murashige and Skoog and N6 basal medium. The highest formation of shoots per responding explant was obtained on N6 medium supplemented with 4.5 µM 2,4-dichlorophenoxy acetic acid in combination with 2.2 µM b...

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Autores principales:	Piña-Escutia,José Luis, Vázquez-García,Luis Miguel, Arzate-Fernández,Amaury Martín
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2010
Materias:	genetic integrity micropropagation oceloxóchitl
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100007
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spelling	oai:scielo:S0717-345820100001000072010-04-28In vitro regeneration and genetic fidelity of Tigridia pavonia (L.f.) DC.Piña-Escutia,José LuisVázquez-García,Luis MiguelArzate-Fernández,Amaury Martín genetic integrity micropropagation oceloxóchitl Plants of Tigridia pavonia (L.f.) DC were regenerated from twin-scaling explants cultured on Murashige and Skoog and N6 basal medium. The highest formation of shoots per responding explant was obtained on N6 medium supplemented with 4.5 µM 2,4-dichlorophenoxy acetic acid in combination with 2.2 µM benzylaminopurine. Shoots rooted readily on N6 basal medium supplemented with 1 g l-1 activated charcoal and 2.6 µM naphtalenacetic acid. The rooted shoots achieved 100% survival. Inter Simple Sequence Repeat analysis was carried out to check for possible genetic alterations in plants obtained after two consecutive subcultures. The results revealed that the recovered plants did not exhibit any type of polymorphism.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.13 n.1 20102010-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100007en10.4067/S0717-34582010000100007
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	genetic integrity micropropagation oceloxóchitl
spellingShingle	genetic integrity micropropagation oceloxóchitl Piña-Escutia,José Luis Vázquez-García,Luis Miguel Arzate-Fernández,Amaury Martín In vitro regeneration and genetic fidelity of Tigridia pavonia (L.f.) DC.
description	Plants of Tigridia pavonia (L.f.) DC were regenerated from twin-scaling explants cultured on Murashige and Skoog and N6 basal medium. The highest formation of shoots per responding explant was obtained on N6 medium supplemented with 4.5 µM 2,4-dichlorophenoxy acetic acid in combination with 2.2 µM benzylaminopurine. Shoots rooted readily on N6 basal medium supplemented with 1 g l-1 activated charcoal and 2.6 µM naphtalenacetic acid. The rooted shoots achieved 100% survival. Inter Simple Sequence Repeat analysis was carried out to check for possible genetic alterations in plants obtained after two consecutive subcultures. The results revealed that the recovered plants did not exhibit any type of polymorphism.
author	Piña-Escutia,José Luis Vázquez-García,Luis Miguel Arzate-Fernández,Amaury Martín
author_facet	Piña-Escutia,José Luis Vázquez-García,Luis Miguel Arzate-Fernández,Amaury Martín
author_sort	Piña-Escutia,José Luis
title	In vitro regeneration and genetic fidelity of Tigridia pavonia (L.f.) DC.
title_short	In vitro regeneration and genetic fidelity of Tigridia pavonia (L.f.) DC.
title_full	In vitro regeneration and genetic fidelity of Tigridia pavonia (L.f.) DC.
title_fullStr	In vitro regeneration and genetic fidelity of Tigridia pavonia (L.f.) DC.
title_full_unstemmed	In vitro regeneration and genetic fidelity of Tigridia pavonia (L.f.) DC.
title_sort	in vitro regeneration and genetic fidelity of tigridia pavonia (l.f.) dc.
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100007
work_keys_str_mv	AT pinaescutiajoseluis invitroregenerationandgeneticfidelityoftigridiapavonialfdc AT vazquezgarcialuismiguel invitroregenerationandgeneticfidelityoftigridiapavonialfdc AT arzatefernandezamaurymartin invitroregenerationandgeneticfidelityoftigridiapavonialfdc
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In vitro regeneration and genetic fidelity of Tigridia pavonia (L.f.) DC.

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