Inhibition of shrimp pathogenic vibrios by extracellular compounds from a proteolytic bacterium Pseudomonas sp. W3

Pseudomonas sp. W3, a bacterium known to produce an extracellular alkaline protease, secreted secondary metabolites that inhibited pathogenic bacteria responsible for shrimp luminous vibriosis disease. Antivibrio compounds in the culture supernatant or culture filtrates (0.45 µm and 0.22 µm) of the...

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Autores principales:	Rattanachuay,Pattamarat, Kantachote,Duangporn, Tantirungkij,Manee, Nitoda,Teruhiko, Kanzaki,Hiroshi
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2010
Materias:	bacteriolytic enzymes bioactive compounds Pseudomonas sp. W3 shrimp pathogenic bacteria Vibrio spp
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100008
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spelling	oai:scielo:S0717-345820100001000082010-04-28Inhibition of shrimp pathogenic vibrios by extracellular compounds from a proteolytic bacterium Pseudomonas sp. W3Rattanachuay,PattamaratKantachote,DuangpornTantirungkij,ManeeNitoda,TeruhikoKanzaki,Hiroshi bacteriolytic enzymes bioactive compounds Pseudomonas sp. W3 shrimp pathogenic bacteria Vibrio spp Pseudomonas sp. W3, a bacterium known to produce an extracellular alkaline protease, secreted secondary metabolites that inhibited pathogenic bacteria responsible for shrimp luminous vibriosis disease. Antivibrio compounds in the culture supernatant or culture filtrates (0.45 µm and 0.22 µm) of the isolate W3 were tested using an agar well diffusion method on a number of pathogenic vibrios. Vibrio harveyi PSU 2015 a pathogenic isolate was the most sensitive strain. The effectiveness of preparations from the isolate W3 against V. harveyi PSU 2015, and V. cholerae PSSCMI 0062 was in the order of culture supernatant > 0.45 µm culture filtrate > 0.22 µm culture filtrate. These extracellular antivibrio compounds also lysed both dead and living cells of V. harveyi PSU 2015. Results of the partial characterization tests indicated that there was some particulate antivibrio compound that was destroyed by treatment with enzymes particularly α-chymotrypsin, autoclaving at 121ºC for 15 min and was mostly removed by filtration through a 0.22 µm filter. Most of the inhibitory compounds were of small molecular weight able to pass through a 0.22 µm filter and were resistant to treatment with various enzymes, pH values between 4-8 and temperatures up to 121ºC for 30 min. The optimum pH for the antivibrio activity in the 0.45 µm culture filtrate was between pH 6-7.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.13 n.1 20102010-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100008en10.4067/S0717-34582010000100008
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	bacteriolytic enzymes bioactive compounds Pseudomonas sp. W3 shrimp pathogenic bacteria Vibrio spp
spellingShingle	bacteriolytic enzymes bioactive compounds Pseudomonas sp. W3 shrimp pathogenic bacteria Vibrio spp Rattanachuay,Pattamarat Kantachote,Duangporn Tantirungkij,Manee Nitoda,Teruhiko Kanzaki,Hiroshi Inhibition of shrimp pathogenic vibrios by extracellular compounds from a proteolytic bacterium Pseudomonas sp. W3
description	Pseudomonas sp. W3, a bacterium known to produce an extracellular alkaline protease, secreted secondary metabolites that inhibited pathogenic bacteria responsible for shrimp luminous vibriosis disease. Antivibrio compounds in the culture supernatant or culture filtrates (0.45 µm and 0.22 µm) of the isolate W3 were tested using an agar well diffusion method on a number of pathogenic vibrios. Vibrio harveyi PSU 2015 a pathogenic isolate was the most sensitive strain. The effectiveness of preparations from the isolate W3 against V. harveyi PSU 2015, and V. cholerae PSSCMI 0062 was in the order of culture supernatant > 0.45 µm culture filtrate > 0.22 µm culture filtrate. These extracellular antivibrio compounds also lysed both dead and living cells of V. harveyi PSU 2015. Results of the partial characterization tests indicated that there was some particulate antivibrio compound that was destroyed by treatment with enzymes particularly α-chymotrypsin, autoclaving at 121ºC for 15 min and was mostly removed by filtration through a 0.22 µm filter. Most of the inhibitory compounds were of small molecular weight able to pass through a 0.22 µm filter and were resistant to treatment with various enzymes, pH values between 4-8 and temperatures up to 121ºC for 30 min. The optimum pH for the antivibrio activity in the 0.45 µm culture filtrate was between pH 6-7.
author	Rattanachuay,Pattamarat Kantachote,Duangporn Tantirungkij,Manee Nitoda,Teruhiko Kanzaki,Hiroshi
author_facet	Rattanachuay,Pattamarat Kantachote,Duangporn Tantirungkij,Manee Nitoda,Teruhiko Kanzaki,Hiroshi
author_sort	Rattanachuay,Pattamarat
title	Inhibition of shrimp pathogenic vibrios by extracellular compounds from a proteolytic bacterium Pseudomonas sp. W3
title_short	Inhibition of shrimp pathogenic vibrios by extracellular compounds from a proteolytic bacterium Pseudomonas sp. W3
title_full	Inhibition of shrimp pathogenic vibrios by extracellular compounds from a proteolytic bacterium Pseudomonas sp. W3
title_fullStr	Inhibition of shrimp pathogenic vibrios by extracellular compounds from a proteolytic bacterium Pseudomonas sp. W3
title_full_unstemmed	Inhibition of shrimp pathogenic vibrios by extracellular compounds from a proteolytic bacterium Pseudomonas sp. W3
title_sort	inhibition of shrimp pathogenic vibrios by extracellular compounds from a proteolytic bacterium pseudomonas sp. w3
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100008
work_keys_str_mv	AT rattanachuaypattamarat inhibitionofshrimppathogenicvibriosbyextracellularcompoundsfromaproteolyticbacteriumpseudomonasspw3 AT kantachoteduangporn inhibitionofshrimppathogenicvibriosbyextracellularcompoundsfromaproteolyticbacteriumpseudomonasspw3 AT tantirungkijmanee inhibitionofshrimppathogenicvibriosbyextracellularcompoundsfromaproteolyticbacteriumpseudomonasspw3 AT nitodateruhiko inhibitionofshrimppathogenicvibriosbyextracellularcompoundsfromaproteolyticbacteriumpseudomonasspw3 AT kanzakihiroshi inhibitionofshrimppathogenicvibriosbyextracellularcompoundsfromaproteolyticbacteriumpseudomonasspw3
_version_	1718441808371908608

Inhibition of shrimp pathogenic vibrios by extracellular compounds from a proteolytic bacterium Pseudomonas sp. W3

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