Molecular authentication of the traditional Chinese medicinal plant Angelica sinensis based on internal transcribed spacer of nrDNA

Traditionally, the authentication of the traditional Chinese medicines (TCM), Angelica sinensis, is based on slightly different morphological characters and complex compounds. Usually, those methods are simultaneously affected by several factors, leading to subtle and ambiguous results. In this stud...

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Autores principales:	Feng,Tu, Liu,Shuang, He,Xing-jin
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2010
Materias:	Angelica sinensis internal transcribed spacer molecular authentication specific primers traditional Chinese medicine
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100009
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spelling	oai:scielo:S0717-345820100001000092010-04-28Molecular authentication of the traditional Chinese medicinal plant Angelica sinensis based on internal transcribed spacer of nrDNAFeng,TuLiu,ShuangHe,Xing-jin Angelica sinensis internal transcribed spacer molecular authentication specific primers traditional Chinese medicine Traditionally, the authentication of the traditional Chinese medicines (TCM), Angelica sinensis, is based on slightly different morphological characters and complex compounds. Usually, those methods are simultaneously affected by several factors, leading to subtle and ambiguous results. In this study, the internal transcribed spacer (ITS) regions of A. sinensis and seven other Angelica species used as adulterants were sequenced. A pair of specific primers was designed from the polymorphic ITS regions to distinguish A. sinensis from the adulterants and regional substitutes. These ITS-derived primers amplified approximately 520 bp specific fragments from the adulterants, whereas no products was amplified with the DNA of A. sinensis. We tested eight commercially crude materials purchased in the market by using these specific primers. The result showed that there were four samples adulterating A. sinensis with regional substitutes. This indicated that A. sinensis could be accurately distinguished from the adulterants and regional substitutes. Therefore, the method of molecular authentication based on the ITS sequences may be contributed to raw material production and quality control of A. sinensis.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.13 n.1 20102010-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100009en10.4067/S0717-34582010000100009
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Angelica sinensis internal transcribed spacer molecular authentication specific primers traditional Chinese medicine
spellingShingle	Angelica sinensis internal transcribed spacer molecular authentication specific primers traditional Chinese medicine Feng,Tu Liu,Shuang He,Xing-jin Molecular authentication of the traditional Chinese medicinal plant Angelica sinensis based on internal transcribed spacer of nrDNA
description	Traditionally, the authentication of the traditional Chinese medicines (TCM), Angelica sinensis, is based on slightly different morphological characters and complex compounds. Usually, those methods are simultaneously affected by several factors, leading to subtle and ambiguous results. In this study, the internal transcribed spacer (ITS) regions of A. sinensis and seven other Angelica species used as adulterants were sequenced. A pair of specific primers was designed from the polymorphic ITS regions to distinguish A. sinensis from the adulterants and regional substitutes. These ITS-derived primers amplified approximately 520 bp specific fragments from the adulterants, whereas no products was amplified with the DNA of A. sinensis. We tested eight commercially crude materials purchased in the market by using these specific primers. The result showed that there were four samples adulterating A. sinensis with regional substitutes. This indicated that A. sinensis could be accurately distinguished from the adulterants and regional substitutes. Therefore, the method of molecular authentication based on the ITS sequences may be contributed to raw material production and quality control of A. sinensis.
author	Feng,Tu Liu,Shuang He,Xing-jin
author_facet	Feng,Tu Liu,Shuang He,Xing-jin
author_sort	Feng,Tu
title	Molecular authentication of the traditional Chinese medicinal plant Angelica sinensis based on internal transcribed spacer of nrDNA
title_short	Molecular authentication of the traditional Chinese medicinal plant Angelica sinensis based on internal transcribed spacer of nrDNA
title_full	Molecular authentication of the traditional Chinese medicinal plant Angelica sinensis based on internal transcribed spacer of nrDNA
title_fullStr	Molecular authentication of the traditional Chinese medicinal plant Angelica sinensis based on internal transcribed spacer of nrDNA
title_full_unstemmed	Molecular authentication of the traditional Chinese medicinal plant Angelica sinensis based on internal transcribed spacer of nrDNA
title_sort	molecular authentication of the traditional chinese medicinal plant angelica sinensis based on internal transcribed spacer of nrdna
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100009
work_keys_str_mv	AT fengtu molecularauthenticationofthetraditionalchinesemedicinalplantangelicasinensisbasedoninternaltranscribedspacerofnrdna AT liushuang molecularauthenticationofthetraditionalchinesemedicinalplantangelicasinensisbasedoninternaltranscribedspacerofnrdna AT hexingjin molecularauthenticationofthetraditionalchinesemedicinalplantangelicasinensisbasedoninternaltranscribedspacerofnrdna
_version_	1718441808682287104

Molecular authentication of the traditional Chinese medicinal plant Angelica sinensis based on internal transcribed spacer of nrDNA

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