Normalized embryoid cDNA library of oil palm (Elaeis guineensis)

A normalized embryoid cDNA library (EON) was constructed based on reassociation kinetics reaction. Results from dot blot hybridization and sequencing of EON cDNA clones clearly indicated that the normalization process reduced the frequency of high abundance transcripts and increased the frequency of...

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Autores principales:	Chan,Pek-Lan, Ma,Lay-Sun, Low,Eng-Ti L, Shariff,Elyana M, Ooi,Leslie Cheng-Li, Cheah,Suan-Choo, Singh,Rajinder
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2010
Materias:	auxin expressed sequence tags gene expression low abundance genes tissue culture
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100010
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id	oai:scielo:S0717-34582010000100010
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spelling	oai:scielo:S0717-345820100001000102010-04-28Normalized embryoid cDNA library of oil palm (Elaeis guineensis)Chan,Pek-LanMa,Lay-SunLow,Eng-Ti LShariff,Elyana MOoi,Leslie Cheng-LiCheah,Suan-ChooSingh,Rajinder auxin expressed sequence tags gene expression low abundance genes tissue culture A normalized embryoid cDNA library (EON) was constructed based on reassociation kinetics reaction. Results from dot blot hybridization and sequencing of EON cDNA clones clearly indicated that the normalization process reduced the frequency of high abundance transcripts and increased the frequency of low abundance gene transcripts. A total of 553 non-redundant expressed sequence tags (ESTs) were identified, 325 of these were not observed in the standard oil palm cDNA libraries sequenced previously. A total of 10 EON cDNA clones were chosen for expression profiling across samples from different stages of the tissue culture process. Two of the genes exhibited promising expression patterns for predicting the embryogenic potential in callus. Some of these genes were also differentially expressed in the various tissues of oil palm. This study showed that normalization of the existing embryoid library improved the chances of identifying transcripts not captured in the standard libraries, some of which could be associated with embryogenesis. This collection of ESTs is particularly well suited for use as candidate genes for development of an oil palm DNA chip, which can be used to obtain a more comprehensive view of the molecular mechanism associated with oil palm tissue culture.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.13 n.1 20102010-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100010en10.4067/S0717-34582010000100010
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	auxin expressed sequence tags gene expression low abundance genes tissue culture
spellingShingle	auxin expressed sequence tags gene expression low abundance genes tissue culture Chan,Pek-Lan Ma,Lay-Sun Low,Eng-Ti L Shariff,Elyana M Ooi,Leslie Cheng-Li Cheah,Suan-Choo Singh,Rajinder Normalized embryoid cDNA library of oil palm (Elaeis guineensis)
description	A normalized embryoid cDNA library (EON) was constructed based on reassociation kinetics reaction. Results from dot blot hybridization and sequencing of EON cDNA clones clearly indicated that the normalization process reduced the frequency of high abundance transcripts and increased the frequency of low abundance gene transcripts. A total of 553 non-redundant expressed sequence tags (ESTs) were identified, 325 of these were not observed in the standard oil palm cDNA libraries sequenced previously. A total of 10 EON cDNA clones were chosen for expression profiling across samples from different stages of the tissue culture process. Two of the genes exhibited promising expression patterns for predicting the embryogenic potential in callus. Some of these genes were also differentially expressed in the various tissues of oil palm. This study showed that normalization of the existing embryoid library improved the chances of identifying transcripts not captured in the standard libraries, some of which could be associated with embryogenesis. This collection of ESTs is particularly well suited for use as candidate genes for development of an oil palm DNA chip, which can be used to obtain a more comprehensive view of the molecular mechanism associated with oil palm tissue culture.
author	Chan,Pek-Lan Ma,Lay-Sun Low,Eng-Ti L Shariff,Elyana M Ooi,Leslie Cheng-Li Cheah,Suan-Choo Singh,Rajinder
author_facet	Chan,Pek-Lan Ma,Lay-Sun Low,Eng-Ti L Shariff,Elyana M Ooi,Leslie Cheng-Li Cheah,Suan-Choo Singh,Rajinder
author_sort	Chan,Pek-Lan
title	Normalized embryoid cDNA library of oil palm (Elaeis guineensis)
title_short	Normalized embryoid cDNA library of oil palm (Elaeis guineensis)
title_full	Normalized embryoid cDNA library of oil palm (Elaeis guineensis)
title_fullStr	Normalized embryoid cDNA library of oil palm (Elaeis guineensis)
title_full_unstemmed	Normalized embryoid cDNA library of oil palm (Elaeis guineensis)
title_sort	normalized embryoid cdna library of oil palm (elaeis guineensis)
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100010
work_keys_str_mv	AT chanpeklan normalizedembryoidcdnalibraryofoilpalmelaeisguineensis AT malaysun normalizedembryoidcdnalibraryofoilpalmelaeisguineensis AT lowengtil normalizedembryoidcdnalibraryofoilpalmelaeisguineensis AT shariffelyanam normalizedembryoidcdnalibraryofoilpalmelaeisguineensis AT ooilesliechengli normalizedembryoidcdnalibraryofoilpalmelaeisguineensis AT cheahsuanchoo normalizedembryoidcdnalibraryofoilpalmelaeisguineensis AT singhrajinder normalizedembryoidcdnalibraryofoilpalmelaeisguineensis
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Normalized embryoid cDNA library of oil palm (Elaeis guineensis)

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