Simultaneous saccharification and fermentation process of different cellulosic substrates using a recombinant Saccharomyces cerevisiae harbouring the β-glucosidase gene
In Brazil, the production of ethanol from sugarcane produces large amounts of lignocellulosic residues (bagasse and straw), which have been driving research and development for the production of second generation ethanol. In the present work, a recombinant Saccharomyces cerevisiae strain expressing...
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Pontificia Universidad Católica de Valparaíso
2010
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oai:scielo:S0717-345820100002000052010-04-28Simultaneous saccharification and fermentation process of different cellulosic substrates using a recombinant Saccharomyces cerevisiae harbouring the β-glucosidase geneFerreira,VerônicaFaber,Mariana de OliveiraMesquita,Sabrina da SilvaPereira Jr,Nei bioethanol lignocellulosic biomass sugarcane bagasse derived cellulignin In Brazil, the production of ethanol from sugarcane produces large amounts of lignocellulosic residues (bagasse and straw), which have been driving research and development for the production of second generation ethanol. In the present work, a recombinant Saccharomyces cerevisiae strain expressing the β-glucosidase gene from Humicola grisea was used for ethanol production from three different cellulosic sources by simultaneous saccharification and fermentation. Initially, a enzymatic pre-hydrolysis step was done with a solid:liquid ratio of 1:4, and an enzymatic load of 25 filter paper activity (FPU).g-1 of cellulosic substrate. Using sugarcane bagasse pretreated cellulignin, crystalline cellulose and carboxymethyl cellulose, 51.7 g L-1, 41.7 g L-1 and 13.8 g L-1 of ethanol was obtained, respectively, at the end of 55 hrs of fermentation. The highest ethanol productivity (0.94 g L-1 hrs-1) was achieved using sugarcane bagasse pretreated cellulignin. The use of a recombinant S. cerevisiae led to extremely low glucose concentrations when compared to other works reported in literature.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.13 n.2 20102010-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000200005en10.4067/S0717-34582010000200005 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
bioethanol lignocellulosic biomass sugarcane bagasse derived cellulignin |
| spellingShingle |
bioethanol lignocellulosic biomass sugarcane bagasse derived cellulignin Ferreira,Verônica Faber,Mariana de Oliveira Mesquita,Sabrina da Silva Pereira Jr,Nei Simultaneous saccharification and fermentation process of different cellulosic substrates using a recombinant Saccharomyces cerevisiae harbouring the β-glucosidase gene |
| description |
In Brazil, the production of ethanol from sugarcane produces large amounts of lignocellulosic residues (bagasse and straw), which have been driving research and development for the production of second generation ethanol. In the present work, a recombinant Saccharomyces cerevisiae strain expressing the β-glucosidase gene from Humicola grisea was used for ethanol production from three different cellulosic sources by simultaneous saccharification and fermentation. Initially, a enzymatic pre-hydrolysis step was done with a solid:liquid ratio of 1:4, and an enzymatic load of 25 filter paper activity (FPU).g-1 of cellulosic substrate. Using sugarcane bagasse pretreated cellulignin, crystalline cellulose and carboxymethyl cellulose, 51.7 g L-1, 41.7 g L-1 and 13.8 g L-1 of ethanol was obtained, respectively, at the end of 55 hrs of fermentation. The highest ethanol productivity (0.94 g L-1 hrs-1) was achieved using sugarcane bagasse pretreated cellulignin. The use of a recombinant S. cerevisiae led to extremely low glucose concentrations when compared to other works reported in literature. |
| author |
Ferreira,Verônica Faber,Mariana de Oliveira Mesquita,Sabrina da Silva Pereira Jr,Nei |
| author_facet |
Ferreira,Verônica Faber,Mariana de Oliveira Mesquita,Sabrina da Silva Pereira Jr,Nei |
| author_sort |
Ferreira,Verônica |
| title |
Simultaneous saccharification and fermentation process of different cellulosic substrates using a recombinant Saccharomyces cerevisiae harbouring the β-glucosidase gene |
| title_short |
Simultaneous saccharification and fermentation process of different cellulosic substrates using a recombinant Saccharomyces cerevisiae harbouring the β-glucosidase gene |
| title_full |
Simultaneous saccharification and fermentation process of different cellulosic substrates using a recombinant Saccharomyces cerevisiae harbouring the β-glucosidase gene |
| title_fullStr |
Simultaneous saccharification and fermentation process of different cellulosic substrates using a recombinant Saccharomyces cerevisiae harbouring the β-glucosidase gene |
| title_full_unstemmed |
Simultaneous saccharification and fermentation process of different cellulosic substrates using a recombinant Saccharomyces cerevisiae harbouring the β-glucosidase gene |
| title_sort |
simultaneous saccharification and fermentation process of different cellulosic substrates using a recombinant saccharomyces cerevisiae harbouring the β-glucosidase gene |
| publisher |
Pontificia Universidad Católica de Valparaíso |
| publishDate |
2010 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000200005 |
| work_keys_str_mv |
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| _version_ |
1718441812370128896 |