Monitoring the threat of unintentional transgene flow into maize gene banks and breeding materials

The use of transgenic crops is steadily increasing around the world, led by soybean (based on total area) and maize (in terms of total number of countries). Transgenic maize is grown in at least 17 countries across four continents: Africa, America, Asia and Europe. The comprehensive global spread of...

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Autores principales: Mezzalama,Monica, Crouch,Jonathan H, Ortiz,Rodomiro
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso 2010
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000200006
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spelling oai:scielo:S0717-345820100002000062010-04-28Monitoring the threat of unintentional transgene flow into maize gene banks and breeding materialsMezzalama,MonicaCrouch,Jonathan HOrtiz,Rodomiro biosafety genetic integrity germplasm enhancement GM-maize The use of transgenic crops is steadily increasing around the world, led by soybean (based on total area) and maize (in terms of total number of countries). Transgenic maize is grown in at least 17 countries across four continents: Africa, America, Asia and Europe. The comprehensive global spread of transgenic maize has significant implications for organizations involved in germplasm conservation and genetic enhancement; particularly as some countries require a GMO-free declaration when receiving shipments of maize germplasm. This article describes the protocol used by the International Maize and Wheat Improvement Center (CIMMYT) for monitoring unintentional transgene flow in maize genebank and breeding plots. The protocol is based on polymerase chain reaction (PCR) markers for detecting specific recombinant DNA sequences in bulked samples collected from sentinel plots. To date, no unintentional transgene flow has been detected in CIMMYT fields of maize genebank accessions or breeding materials.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.13 n.2 20102010-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000200006en10.4067/S0717-34582010000200006
institution Scielo Chile
collection Scielo Chile
language English
topic biosafety
genetic integrity
germplasm enhancement
GM-maize
spellingShingle biosafety
genetic integrity
germplasm enhancement
GM-maize
Mezzalama,Monica
Crouch,Jonathan H
Ortiz,Rodomiro
Monitoring the threat of unintentional transgene flow into maize gene banks and breeding materials
description The use of transgenic crops is steadily increasing around the world, led by soybean (based on total area) and maize (in terms of total number of countries). Transgenic maize is grown in at least 17 countries across four continents: Africa, America, Asia and Europe. The comprehensive global spread of transgenic maize has significant implications for organizations involved in germplasm conservation and genetic enhancement; particularly as some countries require a GMO-free declaration when receiving shipments of maize germplasm. This article describes the protocol used by the International Maize and Wheat Improvement Center (CIMMYT) for monitoring unintentional transgene flow in maize genebank and breeding plots. The protocol is based on polymerase chain reaction (PCR) markers for detecting specific recombinant DNA sequences in bulked samples collected from sentinel plots. To date, no unintentional transgene flow has been detected in CIMMYT fields of maize genebank accessions or breeding materials.
author Mezzalama,Monica
Crouch,Jonathan H
Ortiz,Rodomiro
author_facet Mezzalama,Monica
Crouch,Jonathan H
Ortiz,Rodomiro
author_sort Mezzalama,Monica
title Monitoring the threat of unintentional transgene flow into maize gene banks and breeding materials
title_short Monitoring the threat of unintentional transgene flow into maize gene banks and breeding materials
title_full Monitoring the threat of unintentional transgene flow into maize gene banks and breeding materials
title_fullStr Monitoring the threat of unintentional transgene flow into maize gene banks and breeding materials
title_full_unstemmed Monitoring the threat of unintentional transgene flow into maize gene banks and breeding materials
title_sort monitoring the threat of unintentional transgene flow into maize gene banks and breeding materials
publisher Pontificia Universidad Católica de Valparaíso
publishDate 2010
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000200006
work_keys_str_mv AT mezzalamamonica monitoringthethreatofunintentionaltransgeneflowintomaizegenebanksandbreedingmaterials
AT crouchjonathanh monitoringthethreatofunintentionaltransgeneflowintomaizegenebanksandbreedingmaterials
AT ortizrodomiro monitoringthethreatofunintentionaltransgeneflowintomaizegenebanksandbreedingmaterials
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