Monitoring the threat of unintentional transgene flow into maize gene banks and breeding materials
The use of transgenic crops is steadily increasing around the world, led by soybean (based on total area) and maize (in terms of total number of countries). Transgenic maize is grown in at least 17 countries across four continents: Africa, America, Asia and Europe. The comprehensive global spread of...
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Pontificia Universidad Católica de Valparaíso
2010
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oai:scielo:S0717-345820100002000062010-04-28Monitoring the threat of unintentional transgene flow into maize gene banks and breeding materialsMezzalama,MonicaCrouch,Jonathan HOrtiz,Rodomiro biosafety genetic integrity germplasm enhancement GM-maize The use of transgenic crops is steadily increasing around the world, led by soybean (based on total area) and maize (in terms of total number of countries). Transgenic maize is grown in at least 17 countries across four continents: Africa, America, Asia and Europe. The comprehensive global spread of transgenic maize has significant implications for organizations involved in germplasm conservation and genetic enhancement; particularly as some countries require a GMO-free declaration when receiving shipments of maize germplasm. This article describes the protocol used by the International Maize and Wheat Improvement Center (CIMMYT) for monitoring unintentional transgene flow in maize genebank and breeding plots. The protocol is based on polymerase chain reaction (PCR) markers for detecting specific recombinant DNA sequences in bulked samples collected from sentinel plots. To date, no unintentional transgene flow has been detected in CIMMYT fields of maize genebank accessions or breeding materials.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.13 n.2 20102010-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000200006en10.4067/S0717-34582010000200006 |
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Scielo Chile |
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English |
topic |
biosafety genetic integrity germplasm enhancement GM-maize |
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biosafety genetic integrity germplasm enhancement GM-maize Mezzalama,Monica Crouch,Jonathan H Ortiz,Rodomiro Monitoring the threat of unintentional transgene flow into maize gene banks and breeding materials |
description |
The use of transgenic crops is steadily increasing around the world, led by soybean (based on total area) and maize (in terms of total number of countries). Transgenic maize is grown in at least 17 countries across four continents: Africa, America, Asia and Europe. The comprehensive global spread of transgenic maize has significant implications for organizations involved in germplasm conservation and genetic enhancement; particularly as some countries require a GMO-free declaration when receiving shipments of maize germplasm. This article describes the protocol used by the International Maize and Wheat Improvement Center (CIMMYT) for monitoring unintentional transgene flow in maize genebank and breeding plots. The protocol is based on polymerase chain reaction (PCR) markers for detecting specific recombinant DNA sequences in bulked samples collected from sentinel plots. To date, no unintentional transgene flow has been detected in CIMMYT fields of maize genebank accessions or breeding materials. |
author |
Mezzalama,Monica Crouch,Jonathan H Ortiz,Rodomiro |
author_facet |
Mezzalama,Monica Crouch,Jonathan H Ortiz,Rodomiro |
author_sort |
Mezzalama,Monica |
title |
Monitoring the threat of unintentional transgene flow into maize gene banks and breeding materials |
title_short |
Monitoring the threat of unintentional transgene flow into maize gene banks and breeding materials |
title_full |
Monitoring the threat of unintentional transgene flow into maize gene banks and breeding materials |
title_fullStr |
Monitoring the threat of unintentional transgene flow into maize gene banks and breeding materials |
title_full_unstemmed |
Monitoring the threat of unintentional transgene flow into maize gene banks and breeding materials |
title_sort |
monitoring the threat of unintentional transgene flow into maize gene banks and breeding materials |
publisher |
Pontificia Universidad Católica de Valparaíso |
publishDate |
2010 |
url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000200006 |
work_keys_str_mv |
AT mezzalamamonica monitoringthethreatofunintentionaltransgeneflowintomaizegenebanksandbreedingmaterials AT crouchjonathanh monitoringthethreatofunintentionaltransgeneflowintomaizegenebanksandbreedingmaterials AT ortizrodomiro monitoringthethreatofunintentionaltransgeneflowintomaizegenebanksandbreedingmaterials |
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1718441812831502336 |