Development and application of high-throughput amplified fragment length polymorphism technique in Calluna vulgaris (Ericaceae)

Calluna vulgaris is an important ornamental crop of the horticultural industry in Europe. In order to improve breeding of this species, especially of the most important trait of ‘bud-flowering', the implementation of molecular techniques that allow rapid, reproducible and efficient sc...

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Autores principales:	Borchert,Thomas, Gawenda,Inka
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2010
Materias:	DNA content mapping marker-assisted selection ornamental plant tissue
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000200007
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spelling	oai:scielo:S0717-345820100002000072010-04-28Development and application of high-throughput amplified fragment length polymorphism technique in Calluna vulgaris (Ericaceae)Borchert,ThomasGawenda,Inka DNA content mapping marker-assisted selection ornamental plant tissue Calluna vulgaris is an important ornamental crop of the horticultural industry in Europe. In order to improve breeding of this species, especially of the most important trait of ‘bud-flowering', the implementation of molecular techniques that allow rapid, reproducible and efficient screening of whole segregating populations e.g. for molecular marker and mapping approaches is a requirement. We therefore aimed to introduce the powerful tool of amplified fragment length polymorphisms (AFLP®), a widely and successfully applied method, into our methodological assortment. As an essential prerequisite, the isolated DNA should be of adequate quality which is a common obstacle when dealing with woody species and their interfering secondary components/metabolites. The results of screening different and modified DNA isolation protocols are described. As the outcome of our evaluations of reaction conditions during the AFLP® procedure, we circumstantiate a functional protocol ranging from DNA extraction to visualization of AFLP® banding patterns for the woody crop C. vulgaris. This method is suitable for high throughput genetic applications and may even be transferable to other species. In addition, costs are reduced by reasonable reagents and multiplexing assays.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.13 n.2 20102010-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000200007en10.4067/S0717-34582010000200007
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	DNA content mapping marker-assisted selection ornamental plant tissue
spellingShingle	DNA content mapping marker-assisted selection ornamental plant tissue Borchert,Thomas Gawenda,Inka Development and application of high-throughput amplified fragment length polymorphism technique in Calluna vulgaris (Ericaceae)
description	Calluna vulgaris is an important ornamental crop of the horticultural industry in Europe. In order to improve breeding of this species, especially of the most important trait of ‘bud-flowering', the implementation of molecular techniques that allow rapid, reproducible and efficient screening of whole segregating populations e.g. for molecular marker and mapping approaches is a requirement. We therefore aimed to introduce the powerful tool of amplified fragment length polymorphisms (AFLP®), a widely and successfully applied method, into our methodological assortment. As an essential prerequisite, the isolated DNA should be of adequate quality which is a common obstacle when dealing with woody species and their interfering secondary components/metabolites. The results of screening different and modified DNA isolation protocols are described. As the outcome of our evaluations of reaction conditions during the AFLP® procedure, we circumstantiate a functional protocol ranging from DNA extraction to visualization of AFLP® banding patterns for the woody crop C. vulgaris. This method is suitable for high throughput genetic applications and may even be transferable to other species. In addition, costs are reduced by reasonable reagents and multiplexing assays.
author	Borchert,Thomas Gawenda,Inka
author_facet	Borchert,Thomas Gawenda,Inka
author_sort	Borchert,Thomas
title	Development and application of high-throughput amplified fragment length polymorphism technique in Calluna vulgaris (Ericaceae)
title_short	Development and application of high-throughput amplified fragment length polymorphism technique in Calluna vulgaris (Ericaceae)
title_full	Development and application of high-throughput amplified fragment length polymorphism technique in Calluna vulgaris (Ericaceae)
title_fullStr	Development and application of high-throughput amplified fragment length polymorphism technique in Calluna vulgaris (Ericaceae)
title_full_unstemmed	Development and application of high-throughput amplified fragment length polymorphism technique in Calluna vulgaris (Ericaceae)
title_sort	development and application of high-throughput amplified fragment length polymorphism technique in calluna vulgaris (ericaceae)
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000200007
work_keys_str_mv	AT borchertthomas developmentandapplicationofhighthroughputamplifiedfragmentlengthpolymorphismtechniqueincallunavulgarisericaceae AT gawendainka developmentandapplicationofhighthroughputamplifiedfragmentlengthpolymorphismtechniqueincallunavulgarisericaceae
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Development and application of high-throughput amplified fragment length polymorphism technique in Calluna vulgaris (Ericaceae)

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