Genetic diversity associated with agronomic traits using microsatellite markers in Pakistani rice landraces

Genetic diversity underlies the improvement of crops by plant breeding. Land races of rice (Oryza sativa L.) can contain some valuable alleles not common in modern germplasm. The aim here was to measure genetic diversity and its effect on agronomic traits among rice land-race genotypes grown in Paki...

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Autores principales:	Pervaiz,Zahida Hassan, Rabbani,M. Ashiq, Khaliq,Ishtiaq, Pearce,Stephen R, Malik,Salman A
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2010
Materias:	genetic diversity landraces microsatellite markers Oryza sativa L. Pakistan rice
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300004
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spelling	oai:scielo:S0717-345820100003000042010-06-08Genetic diversity associated with agronomic traits using microsatellite markers in Pakistani rice landracesPervaiz,Zahida HassanRabbani,M. AshiqKhaliq,IshtiaqPearce,Stephen RMalik,Salman A genetic diversity landraces microsatellite markers Oryza sativa L. Pakistan rice Genetic diversity underlies the improvement of crops by plant breeding. Land races of rice (Oryza sativa L.) can contain some valuable alleles not common in modern germplasm. The aim here was to measure genetic diversity and its effect on agronomic traits among rice land-race genotypes grown in Pakistan. Diversity was measured using thirty-five microsatellite markers and seventy-five genotypes. Among the markers used a total of 142 alleles were detected at 32 polymorphic SSR loci, while three loci were monomorphic in Pakistani rice landraces. The number of alleles identified by each marker ranged from 2 to 13 with a mean of 4.4. Size differences between the smallest and largest alleles varied from 11bp to 71bp. Polymorphism information content ranged from 0.124 to 0.836, with an average of 0.569. At nine microsatellite loci, basmati-type landraces amplified more different alleles than those in the coarse-type. DNA markers RM70 and RM72 divided the rice landraces on the basis of days to flowering. A dendrogram based on total microsatellite polymorphism grouped 75 genotypes into four major clusters at 0.40 similarity coefficient, differentiating tall, late maturing and slender aromatic types from the short, early and bold non-aromatic ones. It inferred that Pakistani landraces have diverse genetic bases and can be utilized in future breeding programs. The DNA markers developed will assist in genotype identification, purity testing and plant variety protection.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.13 n.3 20102010-05-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300004en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	genetic diversity landraces microsatellite markers Oryza sativa L. Pakistan rice
spellingShingle	genetic diversity landraces microsatellite markers Oryza sativa L. Pakistan rice Pervaiz,Zahida Hassan Rabbani,M. Ashiq Khaliq,Ishtiaq Pearce,Stephen R Malik,Salman A Genetic diversity associated with agronomic traits using microsatellite markers in Pakistani rice landraces
description	Genetic diversity underlies the improvement of crops by plant breeding. Land races of rice (Oryza sativa L.) can contain some valuable alleles not common in modern germplasm. The aim here was to measure genetic diversity and its effect on agronomic traits among rice land-race genotypes grown in Pakistan. Diversity was measured using thirty-five microsatellite markers and seventy-five genotypes. Among the markers used a total of 142 alleles were detected at 32 polymorphic SSR loci, while three loci were monomorphic in Pakistani rice landraces. The number of alleles identified by each marker ranged from 2 to 13 with a mean of 4.4. Size differences between the smallest and largest alleles varied from 11bp to 71bp. Polymorphism information content ranged from 0.124 to 0.836, with an average of 0.569. At nine microsatellite loci, basmati-type landraces amplified more different alleles than those in the coarse-type. DNA markers RM70 and RM72 divided the rice landraces on the basis of days to flowering. A dendrogram based on total microsatellite polymorphism grouped 75 genotypes into four major clusters at 0.40 similarity coefficient, differentiating tall, late maturing and slender aromatic types from the short, early and bold non-aromatic ones. It inferred that Pakistani landraces have diverse genetic bases and can be utilized in future breeding programs. The DNA markers developed will assist in genotype identification, purity testing and plant variety protection.
author	Pervaiz,Zahida Hassan Rabbani,M. Ashiq Khaliq,Ishtiaq Pearce,Stephen R Malik,Salman A
author_facet	Pervaiz,Zahida Hassan Rabbani,M. Ashiq Khaliq,Ishtiaq Pearce,Stephen R Malik,Salman A
author_sort	Pervaiz,Zahida Hassan
title	Genetic diversity associated with agronomic traits using microsatellite markers in Pakistani rice landraces
title_short	Genetic diversity associated with agronomic traits using microsatellite markers in Pakistani rice landraces
title_full	Genetic diversity associated with agronomic traits using microsatellite markers in Pakistani rice landraces
title_fullStr	Genetic diversity associated with agronomic traits using microsatellite markers in Pakistani rice landraces
title_full_unstemmed	Genetic diversity associated with agronomic traits using microsatellite markers in Pakistani rice landraces
title_sort	genetic diversity associated with agronomic traits using microsatellite markers in pakistani rice landraces
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300004
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Genetic diversity associated with agronomic traits using microsatellite markers in Pakistani rice landraces

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