EST sequencing and SSR marker development from cultivated peanut (Arachis hypogaea L.)

Making use of the gene resources of wild type peanuts is a way to increase the genetic diversity of the cultivars. Marker assisted selection (MAS) could shorten the process of inter-specific hybridization and provide a possible way to remove the undesirable traits. However, the limited number of mol...

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Autores principales:	Song,Guo Qi, Li,Meng Jun, Xiao,Han, Wang,Xing Jun, Tang,Rong Hua, Xia,Han, Zhao,Chuan Zhi, Bi,Yu Ping
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2010
Materias:	Arachis hypogaea L. cultivars EST-SSR polymorphism wild-type peanut
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300007
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spelling	oai:scielo:S0717-345820100003000072010-06-08EST sequencing and SSR marker development from cultivated peanut (Arachis hypogaea L.)Song,Guo QiLi,Meng JunXiao,HanWang,Xing JunTang,Rong HuaXia,HanZhao,Chuan ZhiBi,Yu Ping Arachis hypogaea L. cultivars EST-SSR polymorphism wild-type peanut Making use of the gene resources of wild type peanuts is a way to increase the genetic diversity of the cultivars. Marker assisted selection (MAS) could shorten the process of inter-specific hybridization and provide a possible way to remove the undesirable traits. However, the limited number of molecular markers available in peanut retarded its MAS process. We started a peanut ESTs (Expressed Sequence Tags) project aiming at cloning genes with agronomic importance and developing molecular markers. In this study we found 610 ESTs that contained one or more SSRs from 12,000 peanut ESTs. The most abundant SSRs in peanut are trinucleotides (66.3%) SSRs and followed by dinucleotide (28.8%) SSRs. AG/TC (10.7%) repeat was the most abundant and followed by CT/GA (9.0%), CTT/GAA (7.4%), and AAG/TTC (7.3%) repeats. Ninety-four SSR containing ESTs were randomly selected for primer design and synthesis, of which 33 pairs could generate good amplification and were used for polymorphism assessment. Results showed that polymorphism was very low in cultivars, while high level of polymorphism was revealed in wild type peanuts.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.13 n.3 20102010-05-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300007en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Arachis hypogaea L. cultivars EST-SSR polymorphism wild-type peanut
spellingShingle	Arachis hypogaea L. cultivars EST-SSR polymorphism wild-type peanut Song,Guo Qi Li,Meng Jun Xiao,Han Wang,Xing Jun Tang,Rong Hua Xia,Han Zhao,Chuan Zhi Bi,Yu Ping EST sequencing and SSR marker development from cultivated peanut (Arachis hypogaea L.)
description	Making use of the gene resources of wild type peanuts is a way to increase the genetic diversity of the cultivars. Marker assisted selection (MAS) could shorten the process of inter-specific hybridization and provide a possible way to remove the undesirable traits. However, the limited number of molecular markers available in peanut retarded its MAS process. We started a peanut ESTs (Expressed Sequence Tags) project aiming at cloning genes with agronomic importance and developing molecular markers. In this study we found 610 ESTs that contained one or more SSRs from 12,000 peanut ESTs. The most abundant SSRs in peanut are trinucleotides (66.3%) SSRs and followed by dinucleotide (28.8%) SSRs. AG/TC (10.7%) repeat was the most abundant and followed by CT/GA (9.0%), CTT/GAA (7.4%), and AAG/TTC (7.3%) repeats. Ninety-four SSR containing ESTs were randomly selected for primer design and synthesis, of which 33 pairs could generate good amplification and were used for polymorphism assessment. Results showed that polymorphism was very low in cultivars, while high level of polymorphism was revealed in wild type peanuts.
author	Song,Guo Qi Li,Meng Jun Xiao,Han Wang,Xing Jun Tang,Rong Hua Xia,Han Zhao,Chuan Zhi Bi,Yu Ping
author_facet	Song,Guo Qi Li,Meng Jun Xiao,Han Wang,Xing Jun Tang,Rong Hua Xia,Han Zhao,Chuan Zhi Bi,Yu Ping
author_sort	Song,Guo Qi
title	EST sequencing and SSR marker development from cultivated peanut (Arachis hypogaea L.)
title_short	EST sequencing and SSR marker development from cultivated peanut (Arachis hypogaea L.)
title_full	EST sequencing and SSR marker development from cultivated peanut (Arachis hypogaea L.)
title_fullStr	EST sequencing and SSR marker development from cultivated peanut (Arachis hypogaea L.)
title_full_unstemmed	EST sequencing and SSR marker development from cultivated peanut (Arachis hypogaea L.)
title_sort	est sequencing and ssr marker development from cultivated peanut (arachis hypogaea l.)
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300007
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EST sequencing and SSR marker development from cultivated peanut (Arachis hypogaea L.)

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