Genotypic variation of Agrobacterium-mediated transformation of Italian ryegrass

In the present study, genotypic variation of Agrobacterium-mediated transformation of Korean Italian ryegrass has been evaluated. Mature seed-derived calli of seven cultivars were inoculated and co-cultured with Agrobacterium tumefaciens carrying the binary vector pCAMBIA1301, which contains a repor...

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Autores principales:	Lee,Ki-Won, Choi,Gi Jun, Kim,Ki-Yong, Yoon,Sei Hyung, Ji,Hee Chung, Park,Hyung Soo, Lim,Young Chul, Lee,Sang-Hoon
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2010
Materias:	Agrobacterium forage crop Italian ryegrass transgenic plants
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300008
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spelling	oai:scielo:S0717-345820100003000082010-06-08Genotypic variation of Agrobacterium-mediated transformation of Italian ryegrassLee,Ki-WonChoi,Gi JunKim,Ki-YongYoon,Sei HyungJi,Hee ChungPark,Hyung SooLim,Young ChulLee,Sang-Hoon Agrobacterium forage crop Italian ryegrass transgenic plants In the present study, genotypic variation of Agrobacterium-mediated transformation of Korean Italian ryegrass has been evaluated. Mature seed-derived calli of seven cultivars were inoculated and co-cultured with Agrobacterium tumefaciens carrying the binary vector pCAMBIA1301, which contains a reporter gene (gus) and a plant selectable marker gene conferring resistance to hygromycin (hpt) in the T-DNA region. The effects of several factors such as callus type and callus age on transformation effectiveness and the expression of the GUS gene were investigated. The highest transformation effectiveness (6.7%) was obtained with the Hwasan 101 cultivar when 9-week-old calli (type-I) were inoculated with Agrobacterium. The overall transformation rates of the examined cultivars ranged from 0.4% to 6.7%. GUS histochemical assays, PCR, and southern analysis of transgenic plants demonstrated that transgenes were successfully integrated into the genome of Italian ryegrass. Thus, evaluation of transformation effectiveness and selection of a suitable cultivar of Italian ryegrass may improve molecular breeding of this species.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.13 n.3 20102010-05-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300008en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Agrobacterium forage crop Italian ryegrass transgenic plants
spellingShingle	Agrobacterium forage crop Italian ryegrass transgenic plants Lee,Ki-Won Choi,Gi Jun Kim,Ki-Yong Yoon,Sei Hyung Ji,Hee Chung Park,Hyung Soo Lim,Young Chul Lee,Sang-Hoon Genotypic variation of Agrobacterium-mediated transformation of Italian ryegrass
description	In the present study, genotypic variation of Agrobacterium-mediated transformation of Korean Italian ryegrass has been evaluated. Mature seed-derived calli of seven cultivars were inoculated and co-cultured with Agrobacterium tumefaciens carrying the binary vector pCAMBIA1301, which contains a reporter gene (gus) and a plant selectable marker gene conferring resistance to hygromycin (hpt) in the T-DNA region. The effects of several factors such as callus type and callus age on transformation effectiveness and the expression of the GUS gene were investigated. The highest transformation effectiveness (6.7%) was obtained with the Hwasan 101 cultivar when 9-week-old calli (type-I) were inoculated with Agrobacterium. The overall transformation rates of the examined cultivars ranged from 0.4% to 6.7%. GUS histochemical assays, PCR, and southern analysis of transgenic plants demonstrated that transgenes were successfully integrated into the genome of Italian ryegrass. Thus, evaluation of transformation effectiveness and selection of a suitable cultivar of Italian ryegrass may improve molecular breeding of this species.
author	Lee,Ki-Won Choi,Gi Jun Kim,Ki-Yong Yoon,Sei Hyung Ji,Hee Chung Park,Hyung Soo Lim,Young Chul Lee,Sang-Hoon
author_facet	Lee,Ki-Won Choi,Gi Jun Kim,Ki-Yong Yoon,Sei Hyung Ji,Hee Chung Park,Hyung Soo Lim,Young Chul Lee,Sang-Hoon
author_sort	Lee,Ki-Won
title	Genotypic variation of Agrobacterium-mediated transformation of Italian ryegrass
title_short	Genotypic variation of Agrobacterium-mediated transformation of Italian ryegrass
title_full	Genotypic variation of Agrobacterium-mediated transformation of Italian ryegrass
title_fullStr	Genotypic variation of Agrobacterium-mediated transformation of Italian ryegrass
title_full_unstemmed	Genotypic variation of Agrobacterium-mediated transformation of Italian ryegrass
title_sort	genotypic variation of agrobacterium-mediated transformation of italian ryegrass
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300008
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Genotypic variation of Agrobacterium-mediated transformation of Italian ryegrass

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