Cloning and expression of S-Adenosyl Methionine Synthetase gene in recombinant E. coli strain for large scale production of SAMe

S-Adenosyl Methionine (SAMe) Synthetase is an enzyme which catalyses the synthesis of S-Adenosyl Methionine using methionine and ATP. It is also known as AdoMet which is well known methyl donor, which modifies DNA, RNA, histones and other proteins, dictating replicational, transcriptional and transl...

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Autores principales:	Detchanamurthy,Swaminathan, Shanmugam,Kirubanandan, Parkunan,Salai Madhumathi Arasi, Puttananjaiah,Shilpa, Somasundaram,Balaji, Subramanian,Kannan
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2010
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400001
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spelling	oai:scielo:S0717-345820100004000012010-08-04Cloning and expression of S-Adenosyl Methionine Synthetase gene in recombinant E. coli strain for large scale production of SAMeDetchanamurthy,SwaminathanShanmugam,KirubanandanParkunan,Salai Madhumathi ArasiPuttananjaiah,ShilpaSomasundaram,BalajiSubramanian,KannanS-Adenosyl Methionine (SAMe) Synthetase is an enzyme which catalyses the synthesis of S-Adenosyl Methionine using methionine and ATP. It is also known as AdoMet which is well known methyl donor, which modifies DNA, RNA, histones and other proteins, dictating replicational, transcriptional and translational fidelity, mismatch repair, chromatin modeling, epigenetic modifications and imprinting. The objective of the present work is to clone the SAMe Synthetase gene in recombinant E. coli strain in order to express, characterize and purify it for further synthesis of SAMe in a large scale fermentation. Expression was induced by 1 mM IPTG and expressed protein was characterized by SDS-PAGE. The recombinant E. coli cells were used for the production of SAMe through batch and fed batch fermentation operations. The produced SAMe was purified through paper chromatography in order to use it in our future studies.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.13 n.4 20102010-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400001en
institution	Scielo Chile
collection	Scielo Chile
language	English
description	S-Adenosyl Methionine (SAMe) Synthetase is an enzyme which catalyses the synthesis of S-Adenosyl Methionine using methionine and ATP. It is also known as AdoMet which is well known methyl donor, which modifies DNA, RNA, histones and other proteins, dictating replicational, transcriptional and translational fidelity, mismatch repair, chromatin modeling, epigenetic modifications and imprinting. The objective of the present work is to clone the SAMe Synthetase gene in recombinant E. coli strain in order to express, characterize and purify it for further synthesis of SAMe in a large scale fermentation. Expression was induced by 1 mM IPTG and expressed protein was characterized by SDS-PAGE. The recombinant E. coli cells were used for the production of SAMe through batch and fed batch fermentation operations. The produced SAMe was purified through paper chromatography in order to use it in our future studies.
author	Detchanamurthy,Swaminathan Shanmugam,Kirubanandan Parkunan,Salai Madhumathi Arasi Puttananjaiah,Shilpa Somasundaram,Balaji Subramanian,Kannan
spellingShingle	Detchanamurthy,Swaminathan Shanmugam,Kirubanandan Parkunan,Salai Madhumathi Arasi Puttananjaiah,Shilpa Somasundaram,Balaji Subramanian,Kannan Cloning and expression of S-Adenosyl Methionine Synthetase gene in recombinant E. coli strain for large scale production of SAMe
author_facet	Detchanamurthy,Swaminathan Shanmugam,Kirubanandan Parkunan,Salai Madhumathi Arasi Puttananjaiah,Shilpa Somasundaram,Balaji Subramanian,Kannan
author_sort	Detchanamurthy,Swaminathan
title	Cloning and expression of S-Adenosyl Methionine Synthetase gene in recombinant E. coli strain for large scale production of SAMe
title_short	Cloning and expression of S-Adenosyl Methionine Synthetase gene in recombinant E. coli strain for large scale production of SAMe
title_full	Cloning and expression of S-Adenosyl Methionine Synthetase gene in recombinant E. coli strain for large scale production of SAMe
title_fullStr	Cloning and expression of S-Adenosyl Methionine Synthetase gene in recombinant E. coli strain for large scale production of SAMe
title_full_unstemmed	Cloning and expression of S-Adenosyl Methionine Synthetase gene in recombinant E. coli strain for large scale production of SAMe
title_sort	cloning and expression of s-adenosyl methionine synthetase gene in recombinant e. coli strain for large scale production of same
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400001
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Cloning and expression of S-Adenosyl Methionine Synthetase gene in recombinant E. coli strain for large scale production of SAMe

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