Kinetic characteristics of β-cyclodextrin production by cyclodextrin glycosyltransferase from newly isolated Bacillus sp. C26
The kinetic characteristics of β-cyclodextrin production by a cyclodextrin glycosyltransferase (CGTase) produced by Bacillus sp. C26, a new isolate from a soil sample was investigated. Considering highest yield and initial production rate of β-cyclodextrin, among the starches exami...
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Pontificia Universidad Católica de Valparaíso
2010
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oai:scielo:S0717-345820100004000042010-08-04Kinetic characteristics of β-cyclodextrin production by cyclodextrin glycosyltransferase from newly isolated Bacillus sp. C26Cheirsilp,BenjamasKitcha,SuleepornManeerat,Supasilp Bacillus subtilis ZK8 cell adsorption jiean- peptide response surface methodology wood chips The kinetic characteristics of β-cyclodextrin production by a cyclodextrin glycosyltransferase (CGTase) produced by Bacillus sp. C26, a new isolate from a soil sample was investigated. Considering highest yield and initial production rate of β-cyclodextrin, among the starches examined, soluble starch, tapioca starch, sago starch, corn starch and rice starch, tapioca starch was the best substrate for this CGTase. The optimum temperature for tapioca starch gelatinization prior to its use as a substrate for β-cyclodextrin production was 65ºC. The yield and initial production rate of β-cyclodextrin increased with increasing starch concentration up to 6% and an enzyme concentration up to 48 U/g-starch. The kinetic parameters of Vmax and Km of β-cyclodextrin production from tapioca starch by CGTase were 1.59 mg/mL/h and 22.3 mg/mL, respectively. Considering high initial production rate and high yield of β-cyclodextrin, the optimum reaction temperature was at 50ºC. This study provided the necessary kinetic information that may be useful to define the most suitable condition for industrialized production ofβ-cyclodextrin with the high yield and productivity.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.13 n.4 20102010-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400004en |
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English |
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Bacillus subtilis ZK8 cell adsorption jiean- peptide response surface methodology wood chips |
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Bacillus subtilis ZK8 cell adsorption jiean- peptide response surface methodology wood chips Cheirsilp,Benjamas Kitcha,Suleeporn Maneerat,Supasilp Kinetic characteristics of β-cyclodextrin production by cyclodextrin glycosyltransferase from newly isolated Bacillus sp. C26 |
| description |
The kinetic characteristics of β-cyclodextrin production by a cyclodextrin glycosyltransferase (CGTase) produced by Bacillus sp. C26, a new isolate from a soil sample was investigated. Considering highest yield and initial production rate of β-cyclodextrin, among the starches examined, soluble starch, tapioca starch, sago starch, corn starch and rice starch, tapioca starch was the best substrate for this CGTase. The optimum temperature for tapioca starch gelatinization prior to its use as a substrate for β-cyclodextrin production was 65ºC. The yield and initial production rate of β-cyclodextrin increased with increasing starch concentration up to 6% and an enzyme concentration up to 48 U/g-starch. The kinetic parameters of Vmax and Km of β-cyclodextrin production from tapioca starch by CGTase were 1.59 mg/mL/h and 22.3 mg/mL, respectively. Considering high initial production rate and high yield of β-cyclodextrin, the optimum reaction temperature was at 50ºC. This study provided the necessary kinetic information that may be useful to define the most suitable condition for industrialized production ofβ-cyclodextrin with the high yield and productivity. |
| author |
Cheirsilp,Benjamas Kitcha,Suleeporn Maneerat,Supasilp |
| author_facet |
Cheirsilp,Benjamas Kitcha,Suleeporn Maneerat,Supasilp |
| author_sort |
Cheirsilp,Benjamas |
| title |
Kinetic characteristics of β-cyclodextrin production by cyclodextrin glycosyltransferase from newly isolated Bacillus sp. C26 |
| title_short |
Kinetic characteristics of β-cyclodextrin production by cyclodextrin glycosyltransferase from newly isolated Bacillus sp. C26 |
| title_full |
Kinetic characteristics of β-cyclodextrin production by cyclodextrin glycosyltransferase from newly isolated Bacillus sp. C26 |
| title_fullStr |
Kinetic characteristics of β-cyclodextrin production by cyclodextrin glycosyltransferase from newly isolated Bacillus sp. C26 |
| title_full_unstemmed |
Kinetic characteristics of β-cyclodextrin production by cyclodextrin glycosyltransferase from newly isolated Bacillus sp. C26 |
| title_sort |
kinetic characteristics of β-cyclodextrin production by cyclodextrin glycosyltransferase from newly isolated bacillus sp. c26 |
| publisher |
Pontificia Universidad Católica de Valparaíso |
| publishDate |
2010 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400004 |
| work_keys_str_mv |
AT cheirsilpbenjamas kineticcharacteristicsof946cyclodextrinproductionbycyclodextringlycosyltransferasefromnewlyisolatedbacillusspc26 AT kitchasuleeporn kineticcharacteristicsof946cyclodextrinproductionbycyclodextringlycosyltransferasefromnewlyisolatedbacillusspc26 AT maneeratsupasilp kineticcharacteristicsof946cyclodextrinproductionbycyclodextringlycosyltransferasefromnewlyisolatedbacillusspc26 |
| _version_ |
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