Rapid multiplication and in vitro production of leaf biomass in Kaempferia galanga through tissue culture

An efficient protocol has been established for rapid multiplication and in vitro production of leaf biomass in Kaempferia galanga L, a rare medicinal plant. Different plant growth regulators like Benzyladenine (BA), Indoleacetic acid (IAA), Indolebutyric acid (IBA), Napthaleneacetic acid (NAA) and a...

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Autores principales:	Parida,Reena, Mohanty,Sujata, Kuanar,Ananya, Nayak,Sanghamitra
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2010
Materias:	growth regulators Kaempferia in vitro propagation leaf biomass
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400005
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spelling	oai:scielo:S0717-345820100004000052010-08-04Rapid multiplication and in vitro production of leaf biomass in Kaempferia galanga through tissue cultureParida,ReenaMohanty,SujataKuanar,AnanyaNayak,Sanghamitra growth regulators Kaempferia in vitro propagation leaf biomass An efficient protocol has been established for rapid multiplication and in vitro production of leaf biomass in Kaempferia galanga L, a rare medicinal plant. Different plant growth regulators like Benzyladenine (BA), Indoleacetic acid (IAA), Indolebutyric acid (IBA), Napthaleneacetic acid (NAA) and adenine sulphates (Ads) have been tried for induction of multiple shoots using lateral bud of rhizome as explants. The highest rate of shoot multiplication (11.5 ± 0.6) shoot/explant as well as leaf biomass production (7.4 ± 0.3) gram/explant was observed on Murashige and Skoog medium supplemented with Benzyladenine (1 mg/l) and Indoleacetic acid (0.5 mg/l). Data of shoot multiplication and leaf biomass production were statistically analysed. Upon excission of leaves after 2 months of culture under sterile condition, the base of each plantlet was transferred to fresh media which could produce the same leaf biomass within another 2 months in a 50 ml culture tube containing 20 ml and 250 ml conical flasks containing 30 ml Murashige and Skoog medium. The rate of multiplication and leaf biomass production remained unchanged in subsequent subcultures. The regenerated plantlets were acclimatized in greenhouse and subsequently transferred to the field. Survival rate of the plantlets under ex vitro condition was 95 percent. Genetic fidelity of the regenerants was confirmed using random amplified polymorphic DNA (RAPD) marker. The protocol could be commercially utilized for large scale production of true-to-type plantlets and as an alternative method of leaf biomass production in Kaempferia galanga.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.13 n.4 20102010-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400005en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	growth regulators Kaempferia in vitro propagation leaf biomass
spellingShingle	growth regulators Kaempferia in vitro propagation leaf biomass Parida,Reena Mohanty,Sujata Kuanar,Ananya Nayak,Sanghamitra Rapid multiplication and in vitro production of leaf biomass in Kaempferia galanga through tissue culture
description	An efficient protocol has been established for rapid multiplication and in vitro production of leaf biomass in Kaempferia galanga L, a rare medicinal plant. Different plant growth regulators like Benzyladenine (BA), Indoleacetic acid (IAA), Indolebutyric acid (IBA), Napthaleneacetic acid (NAA) and adenine sulphates (Ads) have been tried for induction of multiple shoots using lateral bud of rhizome as explants. The highest rate of shoot multiplication (11.5 ± 0.6) shoot/explant as well as leaf biomass production (7.4 ± 0.3) gram/explant was observed on Murashige and Skoog medium supplemented with Benzyladenine (1 mg/l) and Indoleacetic acid (0.5 mg/l). Data of shoot multiplication and leaf biomass production were statistically analysed. Upon excission of leaves after 2 months of culture under sterile condition, the base of each plantlet was transferred to fresh media which could produce the same leaf biomass within another 2 months in a 50 ml culture tube containing 20 ml and 250 ml conical flasks containing 30 ml Murashige and Skoog medium. The rate of multiplication and leaf biomass production remained unchanged in subsequent subcultures. The regenerated plantlets were acclimatized in greenhouse and subsequently transferred to the field. Survival rate of the plantlets under ex vitro condition was 95 percent. Genetic fidelity of the regenerants was confirmed using random amplified polymorphic DNA (RAPD) marker. The protocol could be commercially utilized for large scale production of true-to-type plantlets and as an alternative method of leaf biomass production in Kaempferia galanga.
author	Parida,Reena Mohanty,Sujata Kuanar,Ananya Nayak,Sanghamitra
author_facet	Parida,Reena Mohanty,Sujata Kuanar,Ananya Nayak,Sanghamitra
author_sort	Parida,Reena
title	Rapid multiplication and in vitro production of leaf biomass in Kaempferia galanga through tissue culture
title_short	Rapid multiplication and in vitro production of leaf biomass in Kaempferia galanga through tissue culture
title_full	Rapid multiplication and in vitro production of leaf biomass in Kaempferia galanga through tissue culture
title_fullStr	Rapid multiplication and in vitro production of leaf biomass in Kaempferia galanga through tissue culture
title_full_unstemmed	Rapid multiplication and in vitro production of leaf biomass in Kaempferia galanga through tissue culture
title_sort	rapid multiplication and in vitro production of leaf biomass in kaempferia galanga through tissue culture
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400005
work_keys_str_mv	AT paridareena rapidmultiplicationandinvitroproductionofleafbiomassinkaempferiagalangathroughtissueculture AT mohantysujata rapidmultiplicationandinvitroproductionofleafbiomassinkaempferiagalangathroughtissueculture AT kuanarananya rapidmultiplicationandinvitroproductionofleafbiomassinkaempferiagalangathroughtissueculture AT nayaksanghamitra rapidmultiplicationandinvitroproductionofleafbiomassinkaempferiagalangathroughtissueculture
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Rapid multiplication and in vitro production of leaf biomass in Kaempferia galanga through tissue culture

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