Response surface methodology used for statistical optimization of jiean-peptide production by Bacillus subtilis cells adsorbed on wood chips

Response surface methodology (RSM) was used for statistical optimization of jiean-peptide (JAA) production by Bacillus subtilis ZK8 cells adsorbed on wood chips to form a novel fermentation system. The Plackett-Burman design was used in the first step to evaluate the effects of eight factors, includ...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhang,Xiaoyong, Zhou,Jinyan, Fu,Wen, Li,Zhidong, Zhong,Juan, Yang,Jie, Xiao,Liang, Tan,Hong
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2010
Materias:	Bacillus subtilis ZK8 cell adsorption jiean- peptide response surface methodology wood chips
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400006
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-34582010000400006
record_format	dspace
spelling	oai:scielo:S0717-345820100004000062010-08-04Response surface methodology used for statistical optimization of jiean-peptide production by Bacillus subtilis cells adsorbed on wood chipsZhang,XiaoyongZhou,JinyanFu,WenLi,ZhidongZhong,JuanYang,JieXiao,LiangTan,Hong Bacillus subtilis ZK8 cell adsorption jiean- peptide response surface methodology wood chips Response surface methodology (RSM) was used for statistical optimization of jiean-peptide (JAA) production by Bacillus subtilis ZK8 cells adsorbed on wood chips to form a novel fermentation system. The Plackett-Burman design was used in the first step to evaluate the effects of eight factors, including six fermentation medium components and two cell adsorption conditions. Among the variables screened, soybean meal hydrolysate (SMH) and MgSO4·7H2O in the fermentation medium had significanteffects on JAA production. In the second step, the concentrations of SMH and MgSO4·7H2O were further optimized using central composite designs and response surface analysis. The optimized concentration of SMH and MgSO4·7H2O was 24% (v/v) and 0.38% (w/v), respectively, which increased the production of JAA in a shake flask system by 41% relative to optimization of a single variable component of the culture medium.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.13 n.4 20102010-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400006en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Bacillus subtilis ZK8 cell adsorption jiean- peptide response surface methodology wood chips
spellingShingle	Bacillus subtilis ZK8 cell adsorption jiean- peptide response surface methodology wood chips Zhang,Xiaoyong Zhou,Jinyan Fu,Wen Li,Zhidong Zhong,Juan Yang,Jie Xiao,Liang Tan,Hong Response surface methodology used for statistical optimization of jiean-peptide production by Bacillus subtilis cells adsorbed on wood chips
description	Response surface methodology (RSM) was used for statistical optimization of jiean-peptide (JAA) production by Bacillus subtilis ZK8 cells adsorbed on wood chips to form a novel fermentation system. The Plackett-Burman design was used in the first step to evaluate the effects of eight factors, including six fermentation medium components and two cell adsorption conditions. Among the variables screened, soybean meal hydrolysate (SMH) and MgSO4·7H2O in the fermentation medium had significanteffects on JAA production. In the second step, the concentrations of SMH and MgSO4·7H2O were further optimized using central composite designs and response surface analysis. The optimized concentration of SMH and MgSO4·7H2O was 24% (v/v) and 0.38% (w/v), respectively, which increased the production of JAA in a shake flask system by 41% relative to optimization of a single variable component of the culture medium.
author	Zhang,Xiaoyong Zhou,Jinyan Fu,Wen Li,Zhidong Zhong,Juan Yang,Jie Xiao,Liang Tan,Hong
author_facet	Zhang,Xiaoyong Zhou,Jinyan Fu,Wen Li,Zhidong Zhong,Juan Yang,Jie Xiao,Liang Tan,Hong
author_sort	Zhang,Xiaoyong
title	Response surface methodology used for statistical optimization of jiean-peptide production by Bacillus subtilis cells adsorbed on wood chips
title_short	Response surface methodology used for statistical optimization of jiean-peptide production by Bacillus subtilis cells adsorbed on wood chips
title_full	Response surface methodology used for statistical optimization of jiean-peptide production by Bacillus subtilis cells adsorbed on wood chips
title_fullStr	Response surface methodology used for statistical optimization of jiean-peptide production by Bacillus subtilis cells adsorbed on wood chips
title_full_unstemmed	Response surface methodology used for statistical optimization of jiean-peptide production by Bacillus subtilis cells adsorbed on wood chips
title_sort	response surface methodology used for statistical optimization of jiean-peptide production by bacillus subtilis cells adsorbed on wood chips
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400006
work_keys_str_mv	AT zhangxiaoyong responsesurfacemethodologyusedforstatisticaloptimizationofjieanpeptideproductionbybacillussubtiliscellsadsorbedonwoodchips AT zhoujinyan responsesurfacemethodologyusedforstatisticaloptimizationofjieanpeptideproductionbybacillussubtiliscellsadsorbedonwoodchips AT fuwen responsesurfacemethodologyusedforstatisticaloptimizationofjieanpeptideproductionbybacillussubtiliscellsadsorbedonwoodchips AT lizhidong responsesurfacemethodologyusedforstatisticaloptimizationofjieanpeptideproductionbybacillussubtiliscellsadsorbedonwoodchips AT zhongjuan responsesurfacemethodologyusedforstatisticaloptimizationofjieanpeptideproductionbybacillussubtiliscellsadsorbedonwoodchips AT yangjie responsesurfacemethodologyusedforstatisticaloptimizationofjieanpeptideproductionbybacillussubtiliscellsadsorbedonwoodchips AT xiaoliang responsesurfacemethodologyusedforstatisticaloptimizationofjieanpeptideproductionbybacillussubtiliscellsadsorbedonwoodchips AT tanhong responsesurfacemethodologyusedforstatisticaloptimizationofjieanpeptideproductionbybacillussubtiliscellsadsorbedonwoodchips
_version_	1718441817502908416

Response surface methodology used for statistical optimization of jiean-peptide production by Bacillus subtilis cells adsorbed on wood chips

Ejemplares similares