Valorization of raw glycerol for citric acid production by Yarrowia lipolytica yeast

In the present report, citric acid production from raw glycerol in two fed-batch systems by acetate negative-mutants of Yarrowia lipolytica: Wratislavia 1.31 and Wratislavia AWG7 was compared. In the system, in which the total glycerol concentration was 200 g∙L-1, the substrate was added b...

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Autores principales:	Rywinska,Anita, Rymowicz,Waldemar, Marcinkiewicz,Marta
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2010
Materias:	citric acid fed-batch culture Yarrowia lipolytica raw glycerol
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400009
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spelling	oai:scielo:S0717-345820100004000092010-08-04Valorization of raw glycerol for citric acid production by Yarrowia lipolytica yeastRywinska,AnitaRymowicz,WaldemarMarcinkiewicz,Marta citric acid fed-batch culture Yarrowia lipolytica raw glycerol In the present report, citric acid production from raw glycerol in two fed-batch systems by acetate negative-mutants of Yarrowia lipolytica: Wratislavia 1.31 and Wratislavia AWG7 was compared. In the system, in which the total glycerol concentration was 200 g∙L-1, the substrate was added by pulsed additions, and in the other, in which the total glycerol concentration was 300 g∙L-1, the substrate was added at a constant feeding rate of 1.4 g∙h-1. Despite high citric acid concentrations (155.2 and 157.5 g∙L-1 with Y. lipolytica Wratislavia 1.31 and Y. lipolytica Wratislavia AWG7, respectively) obtained from 300 g∙L-1 of glycerol, the yield of citric acid was similar, i.e. about 0.6 g∙g-1. The volumetric citric acid productivity was markedly higher (1.05 and 0.94 g∙L-1h-1 with Y. lipolytica Wratislavia 1.31 and Y. lipolytica Wratislavia AWG7 strains, respectively) in the cultures containing 200 g∙L-1 of carbon source.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.13 n.4 20102010-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400009en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	citric acid fed-batch culture Yarrowia lipolytica raw glycerol
spellingShingle	citric acid fed-batch culture Yarrowia lipolytica raw glycerol Rywinska,Anita Rymowicz,Waldemar Marcinkiewicz,Marta Valorization of raw glycerol for citric acid production by Yarrowia lipolytica yeast
description	In the present report, citric acid production from raw glycerol in two fed-batch systems by acetate negative-mutants of Yarrowia lipolytica: Wratislavia 1.31 and Wratislavia AWG7 was compared. In the system, in which the total glycerol concentration was 200 g∙L-1, the substrate was added by pulsed additions, and in the other, in which the total glycerol concentration was 300 g∙L-1, the substrate was added at a constant feeding rate of 1.4 g∙h-1. Despite high citric acid concentrations (155.2 and 157.5 g∙L-1 with Y. lipolytica Wratislavia 1.31 and Y. lipolytica Wratislavia AWG7, respectively) obtained from 300 g∙L-1 of glycerol, the yield of citric acid was similar, i.e. about 0.6 g∙g-1. The volumetric citric acid productivity was markedly higher (1.05 and 0.94 g∙L-1h-1 with Y. lipolytica Wratislavia 1.31 and Y. lipolytica Wratislavia AWG7 strains, respectively) in the cultures containing 200 g∙L-1 of carbon source.
author	Rywinska,Anita Rymowicz,Waldemar Marcinkiewicz,Marta
author_facet	Rywinska,Anita Rymowicz,Waldemar Marcinkiewicz,Marta
author_sort	Rywinska,Anita
title	Valorization of raw glycerol for citric acid production by Yarrowia lipolytica yeast
title_short	Valorization of raw glycerol for citric acid production by Yarrowia lipolytica yeast
title_full	Valorization of raw glycerol for citric acid production by Yarrowia lipolytica yeast
title_fullStr	Valorization of raw glycerol for citric acid production by Yarrowia lipolytica yeast
title_full_unstemmed	Valorization of raw glycerol for citric acid production by Yarrowia lipolytica yeast
title_sort	valorization of raw glycerol for citric acid production by yarrowia lipolytica yeast
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400009
work_keys_str_mv	AT rywinskaanita valorizationofrawglycerolforcitricacidproductionbyyarrowialipolyticayeast AT rymowiczwaldemar valorizationofrawglycerolforcitricacidproductionbyyarrowialipolyticayeast AT marcinkiewiczmarta valorizationofrawglycerolforcitricacidproductionbyyarrowialipolyticayeast
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Valorization of raw glycerol for citric acid production by Yarrowia lipolytica yeast

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