Variety discrimination of Tigridia pavonia (L.f.) DC. assesed by different length RAPD primers

Tigridia pavonia (L.f.) DC. is one of the important phytogenetic resources of México. This species is used as ornamental, food and medicinal purposes. Despite its ornamental and economic potential, there is little information about the genetic variability. In this study, randomly amplified polymorph...

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Autores principales:	Piña-Escutia,José Luis, Vázquez-García,Luis Miguel, Arzate-Fernández,Amaury Martín
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2010
Materias:	genetic resources RAPD Tigridia pavonia variety discrimination
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400010
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spelling	oai:scielo:S0717-345820100004000102010-08-04Variety discrimination of Tigridia pavonia (L.f.) DC. assesed by different length RAPD primersPiña-Escutia,José LuisVázquez-García,Luis MiguelArzate-Fernández,Amaury Martín genetic resources RAPD Tigridia pavonia variety discrimination Tigridia pavonia (L.f.) DC. is one of the important phytogenetic resources of México. This species is used as ornamental, food and medicinal purposes. Despite its ornamental and economic potential, there is little information about the genetic variability. In this study, randomly amplified polymorphic DNA (RAPD) primers of 10, 15 and 20 bases were used to assess the level of genetic variation among nine botanical varieties of Tigridia pavoniacollected in three localities within State of México. The total number fragments, polymorphic fragments, percentage of polymorphism and resolving power were greater for 15 base (55, 52, 94.5 and 5, respectively) and 20 base (47, 45, 95.7 and 3.8, respectively), in comparison with those obtained from 10 base primers (44, 41, 93.1 and 3.6, respectively).Resultsshowed the major effectiveness of 15 and 20 bases RAPD primers in the genetic differentiation of varieties as compared to 10 bases RAPD primers. The dendrograms based on un-weighted pair group method arithmetic average (UPGMA) analysis of the 10, 15, 20 and the pooled (10, 15 and 20) bases RAPD data were consistent in the clustering varieties, grouping them in two main clusters.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.13 n.4 20102010-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400010en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	genetic resources RAPD Tigridia pavonia variety discrimination
spellingShingle	genetic resources RAPD Tigridia pavonia variety discrimination Piña-Escutia,José Luis Vázquez-García,Luis Miguel Arzate-Fernández,Amaury Martín Variety discrimination of Tigridia pavonia (L.f.) DC. assesed by different length RAPD primers
description	Tigridia pavonia (L.f.) DC. is one of the important phytogenetic resources of México. This species is used as ornamental, food and medicinal purposes. Despite its ornamental and economic potential, there is little information about the genetic variability. In this study, randomly amplified polymorphic DNA (RAPD) primers of 10, 15 and 20 bases were used to assess the level of genetic variation among nine botanical varieties of Tigridia pavoniacollected in three localities within State of México. The total number fragments, polymorphic fragments, percentage of polymorphism and resolving power were greater for 15 base (55, 52, 94.5 and 5, respectively) and 20 base (47, 45, 95.7 and 3.8, respectively), in comparison with those obtained from 10 base primers (44, 41, 93.1 and 3.6, respectively).Resultsshowed the major effectiveness of 15 and 20 bases RAPD primers in the genetic differentiation of varieties as compared to 10 bases RAPD primers. The dendrograms based on un-weighted pair group method arithmetic average (UPGMA) analysis of the 10, 15, 20 and the pooled (10, 15 and 20) bases RAPD data were consistent in the clustering varieties, grouping them in two main clusters.
author	Piña-Escutia,José Luis Vázquez-García,Luis Miguel Arzate-Fernández,Amaury Martín
author_facet	Piña-Escutia,José Luis Vázquez-García,Luis Miguel Arzate-Fernández,Amaury Martín
author_sort	Piña-Escutia,José Luis
title	Variety discrimination of Tigridia pavonia (L.f.) DC. assesed by different length RAPD primers
title_short	Variety discrimination of Tigridia pavonia (L.f.) DC. assesed by different length RAPD primers
title_full	Variety discrimination of Tigridia pavonia (L.f.) DC. assesed by different length RAPD primers
title_fullStr	Variety discrimination of Tigridia pavonia (L.f.) DC. assesed by different length RAPD primers
title_full_unstemmed	Variety discrimination of Tigridia pavonia (L.f.) DC. assesed by different length RAPD primers
title_sort	variety discrimination of tigridia pavonia (l.f.) dc. assesed by different length rapd primers
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400010
work_keys_str_mv	AT pinaescutiajoseluis varietydiscriminationoftigridiapavonialfdcassesedbydifferentlengthrapdprimers AT vazquezgarcialuismiguel varietydiscriminationoftigridiapavonialfdcassesedbydifferentlengthrapdprimers AT arzatefernandezamaurymartin varietydiscriminationoftigridiapavonialfdcassesedbydifferentlengthrapdprimers
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Variety discrimination of Tigridia pavonia (L.f.) DC. assesed by different length RAPD primers

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