Simple method to prepare DNA templates from a slice of peanut cotyledonary tissue for Polymerase Chain Reaction

An efficient DNA extraction method was developed for peanut seed, where only 3-5 mg cotyledonary tissue was enough for more than 50 PCR reactions with a reaction volume of 15 μl. Both low copy number and high copy number DNA sequences were successfully amplified. Processing one seed sample...

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Autores principales:	Yu,Shu Tao, Wang,Chuan Tang, Yu,Shan Lin, Wang,Xiu Zhen, Tang,Yue Yi, Chen,Dian Xu, Zhang,Jian Cheng
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2010
Materias:	cotyledonary tissue DNA extraction groundnut PCR peanut
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400012
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spelling	oai:scielo:S0717-345820100004000122010-08-04Simple method to prepare DNA templates from a slice of peanut cotyledonary tissue for Polymerase Chain ReactionYu,Shu TaoWang,Chuan TangYu,Shan LinWang,Xiu ZhenTang,Yue YiChen,Dian XuZhang,Jian Cheng cotyledonary tissue DNA extraction groundnut PCR peanut An efficient DNA extraction method was developed for peanut seed, where only 3-5 mg cotyledonary tissue was enough for more than 50 PCR reactions with a reaction volume of 15 μl. Both low copy number and high copy number DNA sequences were successfully amplified. Processing one seed sample only took about half an hour. Sampling had no significant effects on germination and development. The DNA extraction method makes it possible to identify transformants and conduct molecular marker studies prior to sowing, and thus may greatly hasten research progress.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.13 n.4 20102010-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400012en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	cotyledonary tissue DNA extraction groundnut PCR peanut
spellingShingle	cotyledonary tissue DNA extraction groundnut PCR peanut Yu,Shu Tao Wang,Chuan Tang Yu,Shan Lin Wang,Xiu Zhen Tang,Yue Yi Chen,Dian Xu Zhang,Jian Cheng Simple method to prepare DNA templates from a slice of peanut cotyledonary tissue for Polymerase Chain Reaction
description	An efficient DNA extraction method was developed for peanut seed, where only 3-5 mg cotyledonary tissue was enough for more than 50 PCR reactions with a reaction volume of 15 μl. Both low copy number and high copy number DNA sequences were successfully amplified. Processing one seed sample only took about half an hour. Sampling had no significant effects on germination and development. The DNA extraction method makes it possible to identify transformants and conduct molecular marker studies prior to sowing, and thus may greatly hasten research progress.
author	Yu,Shu Tao Wang,Chuan Tang Yu,Shan Lin Wang,Xiu Zhen Tang,Yue Yi Chen,Dian Xu Zhang,Jian Cheng
author_facet	Yu,Shu Tao Wang,Chuan Tang Yu,Shan Lin Wang,Xiu Zhen Tang,Yue Yi Chen,Dian Xu Zhang,Jian Cheng
author_sort	Yu,Shu Tao
title	Simple method to prepare DNA templates from a slice of peanut cotyledonary tissue for Polymerase Chain Reaction
title_short	Simple method to prepare DNA templates from a slice of peanut cotyledonary tissue for Polymerase Chain Reaction
title_full	Simple method to prepare DNA templates from a slice of peanut cotyledonary tissue for Polymerase Chain Reaction
title_fullStr	Simple method to prepare DNA templates from a slice of peanut cotyledonary tissue for Polymerase Chain Reaction
title_full_unstemmed	Simple method to prepare DNA templates from a slice of peanut cotyledonary tissue for Polymerase Chain Reaction
title_sort	simple method to prepare dna templates from a slice of peanut cotyledonary tissue for polymerase chain reaction
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400012
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Simple method to prepare DNA templates from a slice of peanut cotyledonary tissue for Polymerase Chain Reaction

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