Simple method to prepare DNA templates from a slice of peanut cotyledonary tissue for Polymerase Chain Reaction

An efficient DNA extraction method was developed for peanut seed, where only 3-5 mg cotyledonary tissue was enough for more than 50 PCR reactions with a reaction volume of 15 μl. Both low copy number and high copy number DNA sequences were successfully amplified. Processing one seed sample...

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Autores principales: Yu,Shu Tao, Wang,Chuan Tang, Yu,Shan Lin, Wang,Xiu Zhen, Tang,Yue Yi, Chen,Dian Xu, Zhang,Jian Cheng
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso 2010
Materias:
PCR
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400012
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