Anticancer and apoptosis-inducing activities of microbial metabolites

The problems of systemic toxicity and drug resistance in cancer chemotherapy urge the continuing discovery of new anticancer agents. We explored the specific anticancer activity from microbial metabolites to find new lead compound. 394 microbial extracts were evaluated on anti-proliferative activity...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Phonnok,Sirinet, Uthaisang-Tanechpongtamb,Wanlaya, Wongsatayanon,Benjamas Thanomsub
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2010
Materias:	anticancer apoptosis bioactive compound caspase microbial product
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500001
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-34582010000500001
record_format	dspace
spelling	oai:scielo:S0717-345820100005000012011-05-24Anticancer and apoptosis-inducing activities of microbial metabolitesPhonnok,SirinetUthaisang-Tanechpongtamb,WanlayaWongsatayanon,Benjamas Thanomsub anticancer apoptosis bioactive compound caspase microbial product The problems of systemic toxicity and drug resistance in cancer chemotherapy urge the continuing discovery of new anticancer agents. We explored the specific anticancer activity from microbial metabolites to find new lead compound. 394 microbial extracts were evaluated on anti-proliferative activity against 4 cancer cell lines using MTT assay. Of these, 20 samples showed varying degree of cytotoxicity but specifically to the cancer cell lines since the growth of normal cells was not significantly inhibited by 1 mg/ml of each cell extracts. The 4 most potent extracts exhibited strongest growth inhibition to each cancer cell type were selected for further studied. Cell morphological changes such as cell shrinkage, lose of surface contact and blebbing were observed in all treated cancer cells. DNA-binding dye staining demonstrated nuclear condensation and fragmentation. Chromosomal DNA cleavage detected as DNA ladder pattern by gel electrophoresis including activation of cellular caspase-3 activity, a hallmark of apoptosis, were observed in all treated cancer cell lines. These characteristics suggested the mechanism of apoptosis cell death induced by the extracts. No growth inhibition and apoptosis characteristic were detected in normal cells even at high concentration used suggesting the selective cytotoxicity and potential candidates to develop as anticancer agents.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.13 n.5 20102010-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500001en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	anticancer apoptosis bioactive compound caspase microbial product
spellingShingle	anticancer apoptosis bioactive compound caspase microbial product Phonnok,Sirinet Uthaisang-Tanechpongtamb,Wanlaya Wongsatayanon,Benjamas Thanomsub Anticancer and apoptosis-inducing activities of microbial metabolites
description	The problems of systemic toxicity and drug resistance in cancer chemotherapy urge the continuing discovery of new anticancer agents. We explored the specific anticancer activity from microbial metabolites to find new lead compound. 394 microbial extracts were evaluated on anti-proliferative activity against 4 cancer cell lines using MTT assay. Of these, 20 samples showed varying degree of cytotoxicity but specifically to the cancer cell lines since the growth of normal cells was not significantly inhibited by 1 mg/ml of each cell extracts. The 4 most potent extracts exhibited strongest growth inhibition to each cancer cell type were selected for further studied. Cell morphological changes such as cell shrinkage, lose of surface contact and blebbing were observed in all treated cancer cells. DNA-binding dye staining demonstrated nuclear condensation and fragmentation. Chromosomal DNA cleavage detected as DNA ladder pattern by gel electrophoresis including activation of cellular caspase-3 activity, a hallmark of apoptosis, were observed in all treated cancer cell lines. These characteristics suggested the mechanism of apoptosis cell death induced by the extracts. No growth inhibition and apoptosis characteristic were detected in normal cells even at high concentration used suggesting the selective cytotoxicity and potential candidates to develop as anticancer agents.
author	Phonnok,Sirinet Uthaisang-Tanechpongtamb,Wanlaya Wongsatayanon,Benjamas Thanomsub
author_facet	Phonnok,Sirinet Uthaisang-Tanechpongtamb,Wanlaya Wongsatayanon,Benjamas Thanomsub
author_sort	Phonnok,Sirinet
title	Anticancer and apoptosis-inducing activities of microbial metabolites
title_short	Anticancer and apoptosis-inducing activities of microbial metabolites
title_full	Anticancer and apoptosis-inducing activities of microbial metabolites
title_fullStr	Anticancer and apoptosis-inducing activities of microbial metabolites
title_full_unstemmed	Anticancer and apoptosis-inducing activities of microbial metabolites
title_sort	anticancer and apoptosis-inducing activities of microbial metabolites
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500001
work_keys_str_mv	AT phonnoksirinet anticancerandapoptosisinducingactivitiesofmicrobialmetabolites AT uthaisangtanechpongtambwanlaya anticancerandapoptosisinducingactivitiesofmicrobialmetabolites AT wongsatayanonbenjamasthanomsub anticancerandapoptosisinducingactivitiesofmicrobialmetabolites
_version_	1718441819547631616

Anticancer and apoptosis-inducing activities of microbial metabolites

Ejemplares similares