Detection of single nucleotide polymorphisms in the conserved ESTs regions of Gossypium arboreum

Exploring genetic variation in Gossypium arboreum L. germplasm is useful as it contains many important genes conferring resistance to different stresses. In limited earlier studies, low level of genetic diversity was found by using conventional DNA marker systems which may impede future genome mappi...

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Autores principales:	Shaheen,Tayyaba, Zafar,Yusuf, Mehboob-ur-Rahman
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2010
Materias:	conserved regions Gossypium arboretum SNPs
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500003
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spelling	oai:scielo:S0717-345820100005000032011-05-24Detection of single nucleotide polymorphisms in the conserved ESTs regions of Gossypium arboreumShaheen,TayyabaZafar,YusufMehboob-ur-Rahman, conserved regions Gossypium arboretum SNPs Exploring genetic variation in Gossypium arboreum L. germplasm is useful as it contains many important genes conferring resistance to different stresses. In limited earlier studies, low level of genetic diversity was found by using conventional DNA marker systems which may impede future genome mapping studies. In the present investigation, we explored the extent of Single Nucleotide Polymorphisms (SNP) among 30 conserved regions of Expressed Sequence Tags (EST) of low copy genes between two genotypes of G. arboreum. A total of 27 SNPs including 21 substitutions and 6 Insertions and deletions (Indels) in 7804 bp were found between these genotypes with a frequency of one SNP per 371 bp and one Indel after every 1300 bp. Out of these SNPs, 52% were transitions, whilst 48% SNPs were transversion. In conclusion, SNPs are expedient markers that can explore polymorphism in highly conserved sequences where other markers are not effective.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.13 n.5 20102010-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500003en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	conserved regions Gossypium arboretum SNPs
spellingShingle	conserved regions Gossypium arboretum SNPs Shaheen,Tayyaba Zafar,Yusuf Mehboob-ur-Rahman, Detection of single nucleotide polymorphisms in the conserved ESTs regions of Gossypium arboreum
description	Exploring genetic variation in Gossypium arboreum L. germplasm is useful as it contains many important genes conferring resistance to different stresses. In limited earlier studies, low level of genetic diversity was found by using conventional DNA marker systems which may impede future genome mapping studies. In the present investigation, we explored the extent of Single Nucleotide Polymorphisms (SNP) among 30 conserved regions of Expressed Sequence Tags (EST) of low copy genes between two genotypes of G. arboreum. A total of 27 SNPs including 21 substitutions and 6 Insertions and deletions (Indels) in 7804 bp were found between these genotypes with a frequency of one SNP per 371 bp and one Indel after every 1300 bp. Out of these SNPs, 52% were transitions, whilst 48% SNPs were transversion. In conclusion, SNPs are expedient markers that can explore polymorphism in highly conserved sequences where other markers are not effective.
author	Shaheen,Tayyaba Zafar,Yusuf Mehboob-ur-Rahman,
author_facet	Shaheen,Tayyaba Zafar,Yusuf Mehboob-ur-Rahman,
author_sort	Shaheen,Tayyaba
title	Detection of single nucleotide polymorphisms in the conserved ESTs regions of Gossypium arboreum
title_short	Detection of single nucleotide polymorphisms in the conserved ESTs regions of Gossypium arboreum
title_full	Detection of single nucleotide polymorphisms in the conserved ESTs regions of Gossypium arboreum
title_fullStr	Detection of single nucleotide polymorphisms in the conserved ESTs regions of Gossypium arboreum
title_full_unstemmed	Detection of single nucleotide polymorphisms in the conserved ESTs regions of Gossypium arboreum
title_sort	detection of single nucleotide polymorphisms in the conserved ests regions of gossypium arboreum
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500003
work_keys_str_mv	AT shaheentayyaba detectionofsinglenucleotidepolymorphismsintheconservedestsregionsofgossypiumarboreum AT zafaryusuf detectionofsinglenucleotidepolymorphismsintheconservedestsregionsofgossypiumarboreum AT mehbooburrahman detectionofsinglenucleotidepolymorphismsintheconservedestsregionsofgossypiumarboreum
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Detection of single nucleotide polymorphisms in the conserved ESTs regions of Gossypium arboreum

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