Isolation and characterization of the tissue and development-specific potato snakin-1 promoter inducible by temperature and wounding

Snakin-1 (StSN1) is a broad-spectrum antimicrobial peptide isolated from Solanum tuberosum. Homologous proteins have been identified in a wide range of species but there is no apparent consensus in the roles they play. A 1394 bp fragment of the 5’upstream region of StSN1 gene, designated P...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Almasia,Natalia I, Narhirñak,Vanesa, Hopp,H. Esteban, Vazquez-Rovere,Cecilia
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2010
Materias:	antimicrobial peptide inducible promoter potato snakin/GASA
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500008
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-34582010000500008
record_format	dspace
spelling	oai:scielo:S0717-345820100005000082011-05-24Isolation and characterization of the tissue and development-specific potato snakin-1 promoter inducible by temperature and woundingAlmasia,Natalia INarhirñak,VanesaHopp,H. EstebanVazquez-Rovere,Cecilia antimicrobial peptide inducible promoter potato snakin/GASA Snakin-1 (StSN1) is a broad-spectrum antimicrobial peptide isolated from Solanum tuberosum. Homologous proteins have been identified in a wide range of species but there is no apparent consensus in the roles they play. A 1394 bp fragment of the 5’upstream region of StSN1 gene, designated PStSN1, was isolated from the potato genome and sequenced. Bioinformatics analyses revealed a total of 55 potential regulatory motifs related to tissue-specificity, stress, defence and hormone responsiveness, among others. PStSN1 spatial and temporal activity was studied in transgenic Arabidopsis plants expressing a reporter gene under this promoter control (PStSN1::GUS). Histochemical staining revealed PStSN1::GUS expression in the root vasculature, cotyledons, young leaves and floral organs. Moreover, GUS staining was detected in young developmental stages gradually decreasing as the plant aged. Stress treatments on transgenic plants showed that PStSN1 activity was induced by high/low temperature and wounding. The characterization of PStSN1 in a model plant establishes a framework for the understanding of its possible biological functions and provides a potential tool for plant modification through genetic engineering.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.13 n.5 20102010-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500008en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	antimicrobial peptide inducible promoter potato snakin/GASA
spellingShingle	antimicrobial peptide inducible promoter potato snakin/GASA Almasia,Natalia I Narhirñak,Vanesa Hopp,H. Esteban Vazquez-Rovere,Cecilia Isolation and characterization of the tissue and development-specific potato snakin-1 promoter inducible by temperature and wounding
description	Snakin-1 (StSN1) is a broad-spectrum antimicrobial peptide isolated from Solanum tuberosum. Homologous proteins have been identified in a wide range of species but there is no apparent consensus in the roles they play. A 1394 bp fragment of the 5’upstream region of StSN1 gene, designated PStSN1, was isolated from the potato genome and sequenced. Bioinformatics analyses revealed a total of 55 potential regulatory motifs related to tissue-specificity, stress, defence and hormone responsiveness, among others. PStSN1 spatial and temporal activity was studied in transgenic Arabidopsis plants expressing a reporter gene under this promoter control (PStSN1::GUS). Histochemical staining revealed PStSN1::GUS expression in the root vasculature, cotyledons, young leaves and floral organs. Moreover, GUS staining was detected in young developmental stages gradually decreasing as the plant aged. Stress treatments on transgenic plants showed that PStSN1 activity was induced by high/low temperature and wounding. The characterization of PStSN1 in a model plant establishes a framework for the understanding of its possible biological functions and provides a potential tool for plant modification through genetic engineering.
author	Almasia,Natalia I Narhirñak,Vanesa Hopp,H. Esteban Vazquez-Rovere,Cecilia
author_facet	Almasia,Natalia I Narhirñak,Vanesa Hopp,H. Esteban Vazquez-Rovere,Cecilia
author_sort	Almasia,Natalia I
title	Isolation and characterization of the tissue and development-specific potato snakin-1 promoter inducible by temperature and wounding
title_short	Isolation and characterization of the tissue and development-specific potato snakin-1 promoter inducible by temperature and wounding
title_full	Isolation and characterization of the tissue and development-specific potato snakin-1 promoter inducible by temperature and wounding
title_fullStr	Isolation and characterization of the tissue and development-specific potato snakin-1 promoter inducible by temperature and wounding
title_full_unstemmed	Isolation and characterization of the tissue and development-specific potato snakin-1 promoter inducible by temperature and wounding
title_sort	isolation and characterization of the tissue and development-specific potato snakin-1 promoter inducible by temperature and wounding
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500008
work_keys_str_mv	AT almasianataliai isolationandcharacterizationofthetissueanddevelopmentspecificpotatosnakin1promoterinduciblebytemperatureandwounding AT narhirnakvanesa isolationandcharacterizationofthetissueanddevelopmentspecificpotatosnakin1promoterinduciblebytemperatureandwounding AT hopphesteban isolationandcharacterizationofthetissueanddevelopmentspecificpotatosnakin1promoterinduciblebytemperatureandwounding AT vazquezroverececilia isolationandcharacterizationofthetissueanddevelopmentspecificpotatosnakin1promoterinduciblebytemperatureandwounding
_version_	1718441821668900864

Isolation and characterization of the tissue and development-specific potato snakin-1 promoter inducible by temperature and wounding

Ejemplares similares