Oxidation of volatile reduced sulphur compounds in biotrickling filter inoculated with Thiobacillus thioparus

Reduced volatile sulphur compounds generate an impact on the environment, because of the bad smell and its low odour threshold. Compared with the existing physicochemical technologies for their elimination, biotrickling filters are an economically and environmentally sustainable alternative. Usually...

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Autores principales:	Cáceres,Manuel, Morales,Marjorie, San Martín,Ricardo, Urrutia,Homero, Aroca,Germán
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2010
Materias:	biofiltration odour removal volatile sulphur reduced compounds
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500011
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spelling	oai:scielo:S0717-345820100005000112011-05-24Oxidation of volatile reduced sulphur compounds in biotrickling filter inoculated with Thiobacillus thioparusCáceres,ManuelMorales,MarjorieSan Martín,RicardoUrrutia,HomeroAroca,Germán biofiltration odour removal volatile sulphur reduced compounds Reduced volatile sulphur compounds generate an impact on the environment, because of the bad smell and its low odour threshold. Compared with the existing physicochemical technologies for their elimination, biotrickling filters are an economically and environmentally sustainable alternative. Usually mixed cultures of microorganisms are used for inoculating biotrickling filters, in this case a pure culture of Thiobacillus thioparus is used for generating a biofilm, allowing to measure its capacity for the oxidation of four volatile reduced sulphur compounds: hydrogen sulphide, dimethyl sulphide, methyl mercaptan and dimethyl disulphide, using a residence time of 0.033 hrs. The viable cells of the biofilm were quantified by epifluorescence microscopy, staining the cells with ethidium bromide and acridine orange, polymerase chain reaction analysis in real time was used for testing the predominance of T. thiopharus in the biofilm. The microorganism was able to adhere and grow on the surface of rings made of polyethylene, with a viable population of 7·10(7) cell·ring-1, a 74% of total cells. The real time PCR showed a persistence of the population of T. thioparus for more than 300 days of operation, without being displaced by other microbial species. The maximum elimination capacities for each compound were 34.4; 21.8; 30.8 and 25.6 gS·m-3·h-1 for H2S, dimethylsulphide, dimethyldisulphide and methyl mercaptan, respectively. We conclude that it is possible to implement a biotrickling filter with the bacteria T. thioparus, which can oxidize volatile reduced sulphur compounds efficiently.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.13 n.5 20102010-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500011en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	biofiltration odour removal volatile sulphur reduced compounds
spellingShingle	biofiltration odour removal volatile sulphur reduced compounds Cáceres,Manuel Morales,Marjorie San Martín,Ricardo Urrutia,Homero Aroca,Germán Oxidation of volatile reduced sulphur compounds in biotrickling filter inoculated with Thiobacillus thioparus
description	Reduced volatile sulphur compounds generate an impact on the environment, because of the bad smell and its low odour threshold. Compared with the existing physicochemical technologies for their elimination, biotrickling filters are an economically and environmentally sustainable alternative. Usually mixed cultures of microorganisms are used for inoculating biotrickling filters, in this case a pure culture of Thiobacillus thioparus is used for generating a biofilm, allowing to measure its capacity for the oxidation of four volatile reduced sulphur compounds: hydrogen sulphide, dimethyl sulphide, methyl mercaptan and dimethyl disulphide, using a residence time of 0.033 hrs. The viable cells of the biofilm were quantified by epifluorescence microscopy, staining the cells with ethidium bromide and acridine orange, polymerase chain reaction analysis in real time was used for testing the predominance of T. thiopharus in the biofilm. The microorganism was able to adhere and grow on the surface of rings made of polyethylene, with a viable population of 7·10(7) cell·ring-1, a 74% of total cells. The real time PCR showed a persistence of the population of T. thioparus for more than 300 days of operation, without being displaced by other microbial species. The maximum elimination capacities for each compound were 34.4; 21.8; 30.8 and 25.6 gS·m-3·h-1 for H2S, dimethylsulphide, dimethyldisulphide and methyl mercaptan, respectively. We conclude that it is possible to implement a biotrickling filter with the bacteria T. thioparus, which can oxidize volatile reduced sulphur compounds efficiently.
author	Cáceres,Manuel Morales,Marjorie San Martín,Ricardo Urrutia,Homero Aroca,Germán
author_facet	Cáceres,Manuel Morales,Marjorie San Martín,Ricardo Urrutia,Homero Aroca,Germán
author_sort	Cáceres,Manuel
title	Oxidation of volatile reduced sulphur compounds in biotrickling filter inoculated with Thiobacillus thioparus
title_short	Oxidation of volatile reduced sulphur compounds in biotrickling filter inoculated with Thiobacillus thioparus
title_full	Oxidation of volatile reduced sulphur compounds in biotrickling filter inoculated with Thiobacillus thioparus
title_fullStr	Oxidation of volatile reduced sulphur compounds in biotrickling filter inoculated with Thiobacillus thioparus
title_full_unstemmed	Oxidation of volatile reduced sulphur compounds in biotrickling filter inoculated with Thiobacillus thioparus
title_sort	oxidation of volatile reduced sulphur compounds in biotrickling filter inoculated with thiobacillus thioparus
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500011
work_keys_str_mv	AT caceresmanuel oxidationofvolatilereducedsulphurcompoundsinbiotricklingfilterinoculatedwiththiobacillusthioparus AT moralesmarjorie oxidationofvolatilereducedsulphurcompoundsinbiotricklingfilterinoculatedwiththiobacillusthioparus AT sanmartinricardo oxidationofvolatilereducedsulphurcompoundsinbiotricklingfilterinoculatedwiththiobacillusthioparus AT urrutiahomero oxidationofvolatilereducedsulphurcompoundsinbiotricklingfilterinoculatedwiththiobacillusthioparus AT arocagerman oxidationofvolatilereducedsulphurcompoundsinbiotricklingfilterinoculatedwiththiobacillusthioparus
_version_	1718441822521393152

Oxidation of volatile reduced sulphur compounds in biotrickling filter inoculated with Thiobacillus thioparus

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