Novel protocol to identify true hybrids in normal oleate x high oleate crosses in peanut
A novel hybrid identification protocol was developed for F0:1 peanut seeds resulting from crosses between normal oleate cultivars with wild type FAD2B gene and high oleate genotypes with an A insertion in FAD2B gene. Presence of a series of overlapped peaks in trace file of the PCR product amplified...
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Pontificia Universidad Católica de Valparaíso
2010
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oai:scielo:S0717-345820100005000182011-05-24Novel protocol to identify true hybrids in normal oleate x high oleate crosses in peanutWang,Chuan TangYu,Shan LinZhang,Shu WeiWang,Xiu ZhenTang,Yue YiZhang,Jian ChengChen,Dian Xu identification NIRS PCR peanut true hybrid A novel hybrid identification protocol was developed for F0:1 peanut seeds resulting from crosses between normal oleate cultivars with wild type FAD2B gene and high oleate genotypes with an A insertion in FAD2B gene. Presence of a series of overlapped peaks in trace file of the PCR product amplified with bF19/R1 primers was an indication of hybridity. This protocol may facilitate high oleate breeding and genetic studies in peanut.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.13 n.5 20102010-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500018en |
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Scielo Chile |
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Scielo Chile |
language |
English |
topic |
identification NIRS PCR peanut true hybrid |
spellingShingle |
identification NIRS PCR peanut true hybrid Wang,Chuan Tang Yu,Shan Lin Zhang,Shu Wei Wang,Xiu Zhen Tang,Yue Yi Zhang,Jian Cheng Chen,Dian Xu Novel protocol to identify true hybrids in normal oleate x high oleate crosses in peanut |
description |
A novel hybrid identification protocol was developed for F0:1 peanut seeds resulting from crosses between normal oleate cultivars with wild type FAD2B gene and high oleate genotypes with an A insertion in FAD2B gene. Presence of a series of overlapped peaks in trace file of the PCR product amplified with bF19/R1 primers was an indication of hybridity. This protocol may facilitate high oleate breeding and genetic studies in peanut. |
author |
Wang,Chuan Tang Yu,Shan Lin Zhang,Shu Wei Wang,Xiu Zhen Tang,Yue Yi Zhang,Jian Cheng Chen,Dian Xu |
author_facet |
Wang,Chuan Tang Yu,Shan Lin Zhang,Shu Wei Wang,Xiu Zhen Tang,Yue Yi Zhang,Jian Cheng Chen,Dian Xu |
author_sort |
Wang,Chuan Tang |
title |
Novel protocol to identify true hybrids in normal oleate x high oleate crosses in peanut |
title_short |
Novel protocol to identify true hybrids in normal oleate x high oleate crosses in peanut |
title_full |
Novel protocol to identify true hybrids in normal oleate x high oleate crosses in peanut |
title_fullStr |
Novel protocol to identify true hybrids in normal oleate x high oleate crosses in peanut |
title_full_unstemmed |
Novel protocol to identify true hybrids in normal oleate x high oleate crosses in peanut |
title_sort |
novel protocol to identify true hybrids in normal oleate x high oleate crosses in peanut |
publisher |
Pontificia Universidad Católica de Valparaíso |
publishDate |
2010 |
url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500018 |
work_keys_str_mv |
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