Novel protocol to identify true hybrids in normal oleate x high oleate crosses in peanut

A novel hybrid identification protocol was developed for F0:1 peanut seeds resulting from crosses between normal oleate cultivars with wild type FAD2B gene and high oleate genotypes with an A insertion in FAD2B gene. Presence of a series of overlapped peaks in trace file of the PCR product amplified...

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Autores principales: Wang,Chuan Tang, Yu,Shan Lin, Zhang,Shu Wei, Wang,Xiu Zhen, Tang,Yue Yi, Zhang,Jian Cheng, Chen,Dian Xu
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso 2010
Materias:
PCR
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500018
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spelling oai:scielo:S0717-345820100005000182011-05-24Novel protocol to identify true hybrids in normal oleate x high oleate crosses in peanutWang,Chuan TangYu,Shan LinZhang,Shu WeiWang,Xiu ZhenTang,Yue YiZhang,Jian ChengChen,Dian Xu identification NIRS PCR peanut true hybrid A novel hybrid identification protocol was developed for F0:1 peanut seeds resulting from crosses between normal oleate cultivars with wild type FAD2B gene and high oleate genotypes with an A insertion in FAD2B gene. Presence of a series of overlapped peaks in trace file of the PCR product amplified with bF19/R1 primers was an indication of hybridity. This protocol may facilitate high oleate breeding and genetic studies in peanut.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.13 n.5 20102010-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500018en
institution Scielo Chile
collection Scielo Chile
language English
topic identification
NIRS
PCR
peanut
true hybrid
spellingShingle identification
NIRS
PCR
peanut
true hybrid
Wang,Chuan Tang
Yu,Shan Lin
Zhang,Shu Wei
Wang,Xiu Zhen
Tang,Yue Yi
Zhang,Jian Cheng
Chen,Dian Xu
Novel protocol to identify true hybrids in normal oleate x high oleate crosses in peanut
description A novel hybrid identification protocol was developed for F0:1 peanut seeds resulting from crosses between normal oleate cultivars with wild type FAD2B gene and high oleate genotypes with an A insertion in FAD2B gene. Presence of a series of overlapped peaks in trace file of the PCR product amplified with bF19/R1 primers was an indication of hybridity. This protocol may facilitate high oleate breeding and genetic studies in peanut.
author Wang,Chuan Tang
Yu,Shan Lin
Zhang,Shu Wei
Wang,Xiu Zhen
Tang,Yue Yi
Zhang,Jian Cheng
Chen,Dian Xu
author_facet Wang,Chuan Tang
Yu,Shan Lin
Zhang,Shu Wei
Wang,Xiu Zhen
Tang,Yue Yi
Zhang,Jian Cheng
Chen,Dian Xu
author_sort Wang,Chuan Tang
title Novel protocol to identify true hybrids in normal oleate x high oleate crosses in peanut
title_short Novel protocol to identify true hybrids in normal oleate x high oleate crosses in peanut
title_full Novel protocol to identify true hybrids in normal oleate x high oleate crosses in peanut
title_fullStr Novel protocol to identify true hybrids in normal oleate x high oleate crosses in peanut
title_full_unstemmed Novel protocol to identify true hybrids in normal oleate x high oleate crosses in peanut
title_sort novel protocol to identify true hybrids in normal oleate x high oleate crosses in peanut
publisher Pontificia Universidad Católica de Valparaíso
publishDate 2010
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500018
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