Enhancing DNA electrotransformation efficiency in Escherichia coli DH10B electrocompetent cells

Enhancing DNA electrotransformation efficiency in Escherichia coli DH10B electrocompetent cells

Electrotransformation also known as electroporation is the most reliable and efficient tool for plasmid DNA uptake. Electrotransformation efficiency is function of many factors which include (1) number of cell washes prior to electroporation, (2) electroporation cell number, (3) electroporation DNA...

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Autores principales: Wu,Ning, Matand,Kanyand, Kebede,Bizuayehu, Acquaah,George, Williams,Sonya
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso 2010
Materias:
cell competency
DNA
E. coli DH10B
electroporation
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500021
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