Breeding of high lipid producing strain of Geotrichum robustum by ion beam implantation

To obtain an industrial strain with high lipid yield, the wild strain G0 of Geotrichum robustum was mutated by means of nitrogen ions implantation. Mutagenic effects of strain G0 by low energy N+ ion implantation were studied. The experimental results indicated that the survival rate curve took a ?s...

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Autores principales:	Cao,Yuanyuan, Yao,Jianming, Li,Jun, Chen,Xiangsong, Wu,Jinyong
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2010
Materias:	fatty acids Geotrichum robustum high lipid producing strain ion beam implantation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600002
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spelling	oai:scielo:S0717-345820100006000022011-05-26Breeding of high lipid producing strain of Geotrichum robustum by ion beam implantationCao,YuanyuanYao,JianmingLi,JunChen,XiangsongWu,Jinyong fatty acids Geotrichum robustum high lipid producing strain ion beam implantation To obtain an industrial strain with high lipid yield, the wild strain G0 of Geotrichum robustum was mutated by means of nitrogen ions implantation. Mutagenic effects of strain G0 by low energy N+ ion implantation were studied. The experimental results indicated that the survival rate curve took a ?saddle? shape, and the positive mutation rate was increased to 22.00% at the dose of nitrogen ions 2.0 x 10(15) ions/cm² when the survival rate was 28.60%. By repeated screening, a high lipid producing strain G9 was obtained. The biomass, lipid content and lipid yield of the mutant can reach 40.25 g/L, 71.14% and 28.63 g/L after cultured in a 5L fermenter for 8 days, increasing by 52.81%, 68.82% and 157.93% to those of wild strain, respectively. Analysis results on fatty acids composition and relative content by gas chromatography and mass spectrometry showed that the lipid in strain G9 was mainly composed of 16-carbon and 18-carbon fatty acids, including 37.360% oleic acid, 23.631% palmitic acid, 4.458% linoleic acid and 26.465% stearic acid. Such compositional features were quite similar to plant oil. Geotrichum robustum strain G9 may be an ideal high lipid producing strain for biodiesel production.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.13 n.6 20102010-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600002en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	fatty acids Geotrichum robustum high lipid producing strain ion beam implantation
spellingShingle	fatty acids Geotrichum robustum high lipid producing strain ion beam implantation Cao,Yuanyuan Yao,Jianming Li,Jun Chen,Xiangsong Wu,Jinyong Breeding of high lipid producing strain of Geotrichum robustum by ion beam implantation
description	To obtain an industrial strain with high lipid yield, the wild strain G0 of Geotrichum robustum was mutated by means of nitrogen ions implantation. Mutagenic effects of strain G0 by low energy N+ ion implantation were studied. The experimental results indicated that the survival rate curve took a ?saddle? shape, and the positive mutation rate was increased to 22.00% at the dose of nitrogen ions 2.0 x 10(15) ions/cm² when the survival rate was 28.60%. By repeated screening, a high lipid producing strain G9 was obtained. The biomass, lipid content and lipid yield of the mutant can reach 40.25 g/L, 71.14% and 28.63 g/L after cultured in a 5L fermenter for 8 days, increasing by 52.81%, 68.82% and 157.93% to those of wild strain, respectively. Analysis results on fatty acids composition and relative content by gas chromatography and mass spectrometry showed that the lipid in strain G9 was mainly composed of 16-carbon and 18-carbon fatty acids, including 37.360% oleic acid, 23.631% palmitic acid, 4.458% linoleic acid and 26.465% stearic acid. Such compositional features were quite similar to plant oil. Geotrichum robustum strain G9 may be an ideal high lipid producing strain for biodiesel production.
author	Cao,Yuanyuan Yao,Jianming Li,Jun Chen,Xiangsong Wu,Jinyong
author_facet	Cao,Yuanyuan Yao,Jianming Li,Jun Chen,Xiangsong Wu,Jinyong
author_sort	Cao,Yuanyuan
title	Breeding of high lipid producing strain of Geotrichum robustum by ion beam implantation
title_short	Breeding of high lipid producing strain of Geotrichum robustum by ion beam implantation
title_full	Breeding of high lipid producing strain of Geotrichum robustum by ion beam implantation
title_fullStr	Breeding of high lipid producing strain of Geotrichum robustum by ion beam implantation
title_full_unstemmed	Breeding of high lipid producing strain of Geotrichum robustum by ion beam implantation
title_sort	breeding of high lipid producing strain of geotrichum robustum by ion beam implantation
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600002
work_keys_str_mv	AT caoyuanyuan breedingofhighlipidproducingstrainofgeotrichumrobustumbyionbeamimplantation AT yaojianming breedingofhighlipidproducingstrainofgeotrichumrobustumbyionbeamimplantation AT lijun breedingofhighlipidproducingstrainofgeotrichumrobustumbyionbeamimplantation AT chenxiangsong breedingofhighlipidproducingstrainofgeotrichumrobustumbyionbeamimplantation AT wujinyong breedingofhighlipidproducingstrainofgeotrichumrobustumbyionbeamimplantation
_version_	1718441825971208192

Breeding of high lipid producing strain of Geotrichum robustum by ion beam implantation

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