Development of trinucleotide (GGC)n SSR markers in peanut (Arachis hypogaea L.)

Cultivated peanut (Arachis hypogaea L.) is an oilseed crop of economic importance. It is native to South America, and it is grown extensively in the semi-arid tropics of Asia, Africa, and Latin America. Given an extremely narrow genetic base, efforts are being made to develop simple sequence repeat...

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Autores principales:	Yuan,Mei, Gong,Limin, Meng,Ronghua, Li,Shuangling, Dang,Phat, Guo,Baozhu, He,Guohao
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2010
Materias:	cultivated peanut microsatellites polymorphism simple sequence repeat
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600005
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spelling	oai:scielo:S0717-345820100006000052011-05-26Development of trinucleotide (GGC)n SSR markers in peanut (Arachis hypogaea L.)Yuan,MeiGong,LiminMeng,RonghuaLi,ShuanglingDang,PhatGuo,BaozhuHe,Guohao cultivated peanut microsatellites polymorphism simple sequence repeat Cultivated peanut (Arachis hypogaea L.) is an oilseed crop of economic importance. It is native to South America, and it is grown extensively in the semi-arid tropics of Asia, Africa, and Latin America. Given an extremely narrow genetic base, efforts are being made to develop simple sequence repeat (SSR) markers to provide useful genetic and genomic tools for the peanut research community. A SSR-enriched library to isolate trinucleotide (GGC)n SSRs in peanut was constructed. A total of 143 unique sequences containing (GGC)n repeats were identified. One hundred thirty eight primer pairs were successfully designed at the flanking regions of SSRs. A suitable polymerase was chosen to amplify these GC-rich sequences. Although a low level of polymorphism was observed in cultivated peanut by these new developed SSRs, a high level of transferability to wild species would be beneficial to increasing the number of SSRs in wild species.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.13 n.6 20102010-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600005en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	cultivated peanut microsatellites polymorphism simple sequence repeat
spellingShingle	cultivated peanut microsatellites polymorphism simple sequence repeat Yuan,Mei Gong,Limin Meng,Ronghua Li,Shuangling Dang,Phat Guo,Baozhu He,Guohao Development of trinucleotide (GGC)n SSR markers in peanut (Arachis hypogaea L.)
description	Cultivated peanut (Arachis hypogaea L.) is an oilseed crop of economic importance. It is native to South America, and it is grown extensively in the semi-arid tropics of Asia, Africa, and Latin America. Given an extremely narrow genetic base, efforts are being made to develop simple sequence repeat (SSR) markers to provide useful genetic and genomic tools for the peanut research community. A SSR-enriched library to isolate trinucleotide (GGC)n SSRs in peanut was constructed. A total of 143 unique sequences containing (GGC)n repeats were identified. One hundred thirty eight primer pairs were successfully designed at the flanking regions of SSRs. A suitable polymerase was chosen to amplify these GC-rich sequences. Although a low level of polymorphism was observed in cultivated peanut by these new developed SSRs, a high level of transferability to wild species would be beneficial to increasing the number of SSRs in wild species.
author	Yuan,Mei Gong,Limin Meng,Ronghua Li,Shuangling Dang,Phat Guo,Baozhu He,Guohao
author_facet	Yuan,Mei Gong,Limin Meng,Ronghua Li,Shuangling Dang,Phat Guo,Baozhu He,Guohao
author_sort	Yuan,Mei
title	Development of trinucleotide (GGC)n SSR markers in peanut (Arachis hypogaea L.)
title_short	Development of trinucleotide (GGC)n SSR markers in peanut (Arachis hypogaea L.)
title_full	Development of trinucleotide (GGC)n SSR markers in peanut (Arachis hypogaea L.)
title_fullStr	Development of trinucleotide (GGC)n SSR markers in peanut (Arachis hypogaea L.)
title_full_unstemmed	Development of trinucleotide (GGC)n SSR markers in peanut (Arachis hypogaea L.)
title_sort	development of trinucleotide (ggc)n ssr markers in peanut (arachis hypogaea l.)
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600005
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Development of trinucleotide (GGC)n SSR markers in peanut (Arachis hypogaea L.)

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