Enhancement with inducers of lacasse production by some strains and application of enzyme to dechlorination of 2,4,5-trichlorophenol

Lacasse is one of the extracellular enzymes excreted from white and brown rot fungi, which is involved in ligninolysis. In the present study, the effects of the addition of lacasse inducers to the medium on enhancement of enzyme production under conditions of submerged fermentation were researched....

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Autores principales:	Gedikli,Serap, Aytar,Pinar, Ünal,Arzu, Yamaç,Mustafa, Çabuk,Ahmet, Kolankaya,Nazif
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2010
Materias:	2,4,5-trichlorophenol basidiomycetes dechlorination inducer lacasse
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600006
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spelling	oai:scielo:S0717-345820100006000062011-05-26Enhancement with inducers of lacasse production by some strains and application of enzyme to dechlorination of 2,4,5-trichlorophenolGedikli,SerapAytar,PinarÜnal,ArzuYamaç,MustafaÇabuk,AhmetKolankaya,Nazif 2,4,5-trichlorophenol basidiomycetes dechlorination inducer lacasse Lacasse is one of the extracellular enzymes excreted from white and brown rot fungi, which is involved in ligninolysis. In the present study, the effects of the addition of lacasse inducers to the medium on enhancement of enzyme production under conditions of submerged fermentation were researched. At first, a culture medium was selected suitable for lacasse production. To increase the production of lacasse using different inducers and to examine the ability of dechlorination, this article focuses on screen lacasse activity of 21 basidiomycetes isolates grown in five culture media. All inducers evaluated influenced lacasse activity positively except for gallic acid, mannitol, and malt extract for studied isolates. Our findings showed that lacasse activity of Trametes versicolor ATCC (200801) when it was induced with wheat bran reached up to 29.08 U ml-1 and was examined the ability of dechlorination of 2,4,5-trichlorophenol (2,4,5-TCP). The parameters including pH, initial substrate concentration, amount of enzyme, period of reaction, and temperature were tested for dechlorination process. Correlation between oxygen consumption and dechlorination processes under the determined optimum conditions was analyzed. Toxicity of 2,4,5-TCP before and after enzymatic treatment was evaluated by Microtox test. The results demonstrated that toxicity of intermediates formed 2,4,5-TCP did not change.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.13 n.6 20102010-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600006en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	2,4,5-trichlorophenol basidiomycetes dechlorination inducer lacasse
spellingShingle	2,4,5-trichlorophenol basidiomycetes dechlorination inducer lacasse Gedikli,Serap Aytar,Pinar Ünal,Arzu Yamaç,Mustafa Çabuk,Ahmet Kolankaya,Nazif Enhancement with inducers of lacasse production by some strains and application of enzyme to dechlorination of 2,4,5-trichlorophenol
description	Lacasse is one of the extracellular enzymes excreted from white and brown rot fungi, which is involved in ligninolysis. In the present study, the effects of the addition of lacasse inducers to the medium on enhancement of enzyme production under conditions of submerged fermentation were researched. At first, a culture medium was selected suitable for lacasse production. To increase the production of lacasse using different inducers and to examine the ability of dechlorination, this article focuses on screen lacasse activity of 21 basidiomycetes isolates grown in five culture media. All inducers evaluated influenced lacasse activity positively except for gallic acid, mannitol, and malt extract for studied isolates. Our findings showed that lacasse activity of Trametes versicolor ATCC (200801) when it was induced with wheat bran reached up to 29.08 U ml-1 and was examined the ability of dechlorination of 2,4,5-trichlorophenol (2,4,5-TCP). The parameters including pH, initial substrate concentration, amount of enzyme, period of reaction, and temperature were tested for dechlorination process. Correlation between oxygen consumption and dechlorination processes under the determined optimum conditions was analyzed. Toxicity of 2,4,5-TCP before and after enzymatic treatment was evaluated by Microtox test. The results demonstrated that toxicity of intermediates formed 2,4,5-TCP did not change.
author	Gedikli,Serap Aytar,Pinar Ünal,Arzu Yamaç,Mustafa Çabuk,Ahmet Kolankaya,Nazif
author_facet	Gedikli,Serap Aytar,Pinar Ünal,Arzu Yamaç,Mustafa Çabuk,Ahmet Kolankaya,Nazif
author_sort	Gedikli,Serap
title	Enhancement with inducers of lacasse production by some strains and application of enzyme to dechlorination of 2,4,5-trichlorophenol
title_short	Enhancement with inducers of lacasse production by some strains and application of enzyme to dechlorination of 2,4,5-trichlorophenol
title_full	Enhancement with inducers of lacasse production by some strains and application of enzyme to dechlorination of 2,4,5-trichlorophenol
title_fullStr	Enhancement with inducers of lacasse production by some strains and application of enzyme to dechlorination of 2,4,5-trichlorophenol
title_full_unstemmed	Enhancement with inducers of lacasse production by some strains and application of enzyme to dechlorination of 2,4,5-trichlorophenol
title_sort	enhancement with inducers of lacasse production by some strains and application of enzyme to dechlorination of 2,4,5-trichlorophenol
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600006
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Enhancement with inducers of lacasse production by some strains and application of enzyme to dechlorination of 2,4,5-trichlorophenol

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