Genetic mapping of EST-SSR, SSR and InDel to improve saturation of genomic regions in a previously developed sunflower map

In order to saturate a sunflower genetic map and facilitate marker-assisted selection (MAS) breeding for stress response, it is necessary to enhance map saturation with molecular markers localized in linkage groups associated to genomic regions involved in these traits. This work describes the ident...

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Autores principales:	Talia,Paola, Nishinakamasu,Verónica, Hopp,Horacio Esteban, Heinz,Ruth Amelia, Paniego,Norma
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2010
Materias:	EST-SSR InDels linkage map SSR sunflower
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600007
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spelling	oai:scielo:S0717-345820100006000072011-05-26Genetic mapping of EST-SSR, SSR and InDel to improve saturation of genomic regions in a previously developed sunflower mapTalia,PaolaNishinakamasu,VerónicaHopp,Horacio EstebanHeinz,Ruth AmeliaPaniego,Norma EST-SSR InDels linkage map SSR sunflower In order to saturate a sunflower genetic map and facilitate marker-assisted selection (MAS) breeding for stress response, it is necessary to enhance map saturation with molecular markers localized in linkage groups associated to genomic regions involved in these traits. This work describes the identification and characterization of 1,134 simple sequence repeat (SSR) containing expressed sequence tags (EST) from unigenes available databases. Twelve of these functional markers as well as 41 public SSR markers were successfully localized in linkage groups, thus contributing to the saturation of specific regions on a reference genetic-linkage-map derived from recombinant inbred lines (RIL) mapping population from the cross between PAC2 x RHA266 lines. The enriched map includes 547 markers (231 SSR, 9 EST-SSR, 3 insertions/deletions (InDel) and 304 amplified fragment length polymorphisms (AFLP) distributed in 17 linkage groups (LG), spanning genetic size to 1,942.3 cM and improving its mean density to 3.6 cM per locus. As consequence, no gaps longer than 13.2 cM remain uncovered throughout the entire map, which increases the feasibility of detecting genes or traits of agronomic importance in sunflower.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.13 n.6 20102010-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600007en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	EST-SSR InDels linkage map SSR sunflower
spellingShingle	EST-SSR InDels linkage map SSR sunflower Talia,Paola Nishinakamasu,Verónica Hopp,Horacio Esteban Heinz,Ruth Amelia Paniego,Norma Genetic mapping of EST-SSR, SSR and InDel to improve saturation of genomic regions in a previously developed sunflower map
description	In order to saturate a sunflower genetic map and facilitate marker-assisted selection (MAS) breeding for stress response, it is necessary to enhance map saturation with molecular markers localized in linkage groups associated to genomic regions involved in these traits. This work describes the identification and characterization of 1,134 simple sequence repeat (SSR) containing expressed sequence tags (EST) from unigenes available databases. Twelve of these functional markers as well as 41 public SSR markers were successfully localized in linkage groups, thus contributing to the saturation of specific regions on a reference genetic-linkage-map derived from recombinant inbred lines (RIL) mapping population from the cross between PAC2 x RHA266 lines. The enriched map includes 547 markers (231 SSR, 9 EST-SSR, 3 insertions/deletions (InDel) and 304 amplified fragment length polymorphisms (AFLP) distributed in 17 linkage groups (LG), spanning genetic size to 1,942.3 cM and improving its mean density to 3.6 cM per locus. As consequence, no gaps longer than 13.2 cM remain uncovered throughout the entire map, which increases the feasibility of detecting genes or traits of agronomic importance in sunflower.
author	Talia,Paola Nishinakamasu,Verónica Hopp,Horacio Esteban Heinz,Ruth Amelia Paniego,Norma
author_facet	Talia,Paola Nishinakamasu,Verónica Hopp,Horacio Esteban Heinz,Ruth Amelia Paniego,Norma
author_sort	Talia,Paola
title	Genetic mapping of EST-SSR, SSR and InDel to improve saturation of genomic regions in a previously developed sunflower map
title_short	Genetic mapping of EST-SSR, SSR and InDel to improve saturation of genomic regions in a previously developed sunflower map
title_full	Genetic mapping of EST-SSR, SSR and InDel to improve saturation of genomic regions in a previously developed sunflower map
title_fullStr	Genetic mapping of EST-SSR, SSR and InDel to improve saturation of genomic regions in a previously developed sunflower map
title_full_unstemmed	Genetic mapping of EST-SSR, SSR and InDel to improve saturation of genomic regions in a previously developed sunflower map
title_sort	genetic mapping of est-ssr, ssr and indel to improve saturation of genomic regions in a previously developed sunflower map
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600007
work_keys_str_mv	AT taliapaola geneticmappingofestssrssrandindeltoimprovesaturationofgenomicregionsinapreviouslydevelopedsunflowermap AT nishinakamasuveronica geneticmappingofestssrssrandindeltoimprovesaturationofgenomicregionsinapreviouslydevelopedsunflowermap AT hopphoracioesteban geneticmappingofestssrssrandindeltoimprovesaturationofgenomicregionsinapreviouslydevelopedsunflowermap AT heinzruthamelia geneticmappingofestssrssrandindeltoimprovesaturationofgenomicregionsinapreviouslydevelopedsunflowermap AT paniegonorma geneticmappingofestssrssrandindeltoimprovesaturationofgenomicregionsinapreviouslydevelopedsunflowermap
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Genetic mapping of EST-SSR, SSR and InDel to improve saturation of genomic regions in a previously developed sunflower map

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