Marker-assisted backcrossing using microsatellites and validation of SCAR Phs marker for resistance to white mold in common bean

Molecular markers may accelerate selection through the identification of plants with higher proportion of recurrent parent genome, as well as identifying those plants bearing target alleles like quantitative traits loci (QTLs) for white mold resistance. The objectives of this work were: 1) to employ...

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Autores principales:	Carneiro,Flávia Fernandes, Santos,João Bosco dos, Leite,Monik Evelin
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2010
Materias:	backcross genetic similarity Phaseolus vulgaris SCAR Sclerotinia sclerotiorum SSR assisted selection
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600009
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spelling	oai:scielo:S0717-345820100006000092011-05-26Marker-assisted backcrossing using microsatellites and validation of SCAR Phs marker for resistance to white mold in common beanCarneiro,Flávia FernandesSantos,João Bosco dosLeite,Monik Evelin backcross genetic similarity Phaseolus vulgaris SCAR Sclerotinia sclerotiorum SSR assisted selection Molecular markers may accelerate selection through the identification of plants with higher proportion of recurrent parent genome, as well as identifying those plants bearing target alleles like quantitative traits loci (QTLs) for white mold resistance. The objectives of this work were: 1) to employ microsatellite markers (SSR) in order to accelerate the recovery of recurrent parent genome 2) to validate sequence characterized amplified region (SCAR) Phs associated with a QTL that confers resistance to white mold, as previously identified in bean populations. Lines G122 and M20 were crossed, which generated 267 F1 plants from backcross (BC) BC1 and 113 plants from backcross BC2.SSR polymorphic markers were adopted. The relationship between BC plants and the recurrent parent was estimated based on the recurrent genome proportion (PR) in each BC plant, and the Sorensen-Dice genetic similarity (sg ir). To determine how much the phenotypic variation is explained by SCAR Phs, 56 F1:2BC1 progenies were evaluated on the field following a random block design with two replications through the straw test method. SSR markers are efficient in identifying individuals with a greater proportion of the recurrent genome. SCAR Phs was not efficient for the indirect selection of common beans for white mold resistance.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.13 n.6 20102010-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600009en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	backcross genetic similarity Phaseolus vulgaris SCAR Sclerotinia sclerotiorum SSR assisted selection
spellingShingle	backcross genetic similarity Phaseolus vulgaris SCAR Sclerotinia sclerotiorum SSR assisted selection Carneiro,Flávia Fernandes Santos,João Bosco dos Leite,Monik Evelin Marker-assisted backcrossing using microsatellites and validation of SCAR Phs marker for resistance to white mold in common bean
description	Molecular markers may accelerate selection through the identification of plants with higher proportion of recurrent parent genome, as well as identifying those plants bearing target alleles like quantitative traits loci (QTLs) for white mold resistance. The objectives of this work were: 1) to employ microsatellite markers (SSR) in order to accelerate the recovery of recurrent parent genome 2) to validate sequence characterized amplified region (SCAR) Phs associated with a QTL that confers resistance to white mold, as previously identified in bean populations. Lines G122 and M20 were crossed, which generated 267 F1 plants from backcross (BC) BC1 and 113 plants from backcross BC2.SSR polymorphic markers were adopted. The relationship between BC plants and the recurrent parent was estimated based on the recurrent genome proportion (PR) in each BC plant, and the Sorensen-Dice genetic similarity (sg ir). To determine how much the phenotypic variation is explained by SCAR Phs, 56 F1:2BC1 progenies were evaluated on the field following a random block design with two replications through the straw test method. SSR markers are efficient in identifying individuals with a greater proportion of the recurrent genome. SCAR Phs was not efficient for the indirect selection of common beans for white mold resistance.
author	Carneiro,Flávia Fernandes Santos,João Bosco dos Leite,Monik Evelin
author_facet	Carneiro,Flávia Fernandes Santos,João Bosco dos Leite,Monik Evelin
author_sort	Carneiro,Flávia Fernandes
title	Marker-assisted backcrossing using microsatellites and validation of SCAR Phs marker for resistance to white mold in common bean
title_short	Marker-assisted backcrossing using microsatellites and validation of SCAR Phs marker for resistance to white mold in common bean
title_full	Marker-assisted backcrossing using microsatellites and validation of SCAR Phs marker for resistance to white mold in common bean
title_fullStr	Marker-assisted backcrossing using microsatellites and validation of SCAR Phs marker for resistance to white mold in common bean
title_full_unstemmed	Marker-assisted backcrossing using microsatellites and validation of SCAR Phs marker for resistance to white mold in common bean
title_sort	marker-assisted backcrossing using microsatellites and validation of scar phs marker for resistance to white mold in common bean
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600009
work_keys_str_mv	AT carneiroflaviafernandes markerassistedbackcrossingusingmicrosatellitesandvalidationofscarphsmarkerforresistancetowhitemoldincommonbean AT santosjoaoboscodos markerassistedbackcrossingusingmicrosatellitesandvalidationofscarphsmarkerforresistancetowhitemoldincommonbean AT leitemonikevelin markerassistedbackcrossingusingmicrosatellitesandvalidationofscarphsmarkerforresistancetowhitemoldincommonbean
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Marker-assisted backcrossing using microsatellites and validation of SCAR Phs marker for resistance to white mold in common bean

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