Molecular characterization and transcriptome profiling of expansin genes isolated from Calotropis procera fibers

The Calotropis procera seed fibers provide an excellent model system to study the genes involved in fiber elongation, fineness and strength. Expansins constitute one of the important gene families involved in plant cell expansion and other cell wall modification processes. Four homologs of Expansin...

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Autores principales:	Cheema,Hafiza Masooma Naseer, Bashir,Aftab, Khatoon,Asia, Iqbal,Nadia, Zafar,Yusuf, Malik,Kauser A
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2010
Materias:	Calotropis procera cDNA library cotton fibers EXPANSIN A signal peptide transcriptome profiling
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600010
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spelling	oai:scielo:S0717-345820100006000102011-05-26Molecular characterization and transcriptome profiling of expansin genes isolated from Calotropis procera fibersCheema,Hafiza Masooma NaseerBashir,AftabKhatoon,AsiaIqbal,NadiaZafar,YusufMalik,Kauser A Calotropis procera cDNA library cotton fibers EXPANSIN A signal peptide transcriptome profiling The Calotropis procera seed fibers provide an excellent model system to study the genes involved in fiber elongation, fineness and strength. Expansins constitute one of the important gene families involved in plant cell expansion and other cell wall modification processes. Four homologs of Expansin A gene i.e. CpEXPA1, CpEXPA2, CpEXPA3 and CpEXPA4 were isolated from the cDNA library obtained from fast growing Calotropis procera fibers. These homologs represented typical Expansin A family. Each of them had two conserved domains including GH45 like domain and the putative polysaccharide binding domain. The deduced amino acid sequences of the homologs indicated three conserved motifs: i) eight cysteine residues at N-terminus, ii) four tryptophan residues at C-terminus and iii) a Histidine-Phenylalanine-Aspartate motif in the center of the sequence. The presence of N-terminal signal peptide consisting of hydrophobic amino acids and a transmembrane region in all these expansin isoforms suggests their cotranslational insertion into the endoplasmic reticulum and then transportation to the cell wall by secretory pathway. The relative quantification of the four expansins in root, stem, fiber and leave tissues indicated that the transcripts of CpEXPA1, CpEXPA2, CpEXPA3 and CpEXPA4 are variably transcribed in these tissues. The lowest transcription of all the four Expansin A isoforms was observed in elongating roots indicating that root tissue might be having specific expansins other than those confined to air grown organs.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.13 n.6 20102010-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600010en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Calotropis procera cDNA library cotton fibers EXPANSIN A signal peptide transcriptome profiling
spellingShingle	Calotropis procera cDNA library cotton fibers EXPANSIN A signal peptide transcriptome profiling Cheema,Hafiza Masooma Naseer Bashir,Aftab Khatoon,Asia Iqbal,Nadia Zafar,Yusuf Malik,Kauser A Molecular characterization and transcriptome profiling of expansin genes isolated from Calotropis procera fibers
description	The Calotropis procera seed fibers provide an excellent model system to study the genes involved in fiber elongation, fineness and strength. Expansins constitute one of the important gene families involved in plant cell expansion and other cell wall modification processes. Four homologs of Expansin A gene i.e. CpEXPA1, CpEXPA2, CpEXPA3 and CpEXPA4 were isolated from the cDNA library obtained from fast growing Calotropis procera fibers. These homologs represented typical Expansin A family. Each of them had two conserved domains including GH45 like domain and the putative polysaccharide binding domain. The deduced amino acid sequences of the homologs indicated three conserved motifs: i) eight cysteine residues at N-terminus, ii) four tryptophan residues at C-terminus and iii) a Histidine-Phenylalanine-Aspartate motif in the center of the sequence. The presence of N-terminal signal peptide consisting of hydrophobic amino acids and a transmembrane region in all these expansin isoforms suggests their cotranslational insertion into the endoplasmic reticulum and then transportation to the cell wall by secretory pathway. The relative quantification of the four expansins in root, stem, fiber and leave tissues indicated that the transcripts of CpEXPA1, CpEXPA2, CpEXPA3 and CpEXPA4 are variably transcribed in these tissues. The lowest transcription of all the four Expansin A isoforms was observed in elongating roots indicating that root tissue might be having specific expansins other than those confined to air grown organs.
author	Cheema,Hafiza Masooma Naseer Bashir,Aftab Khatoon,Asia Iqbal,Nadia Zafar,Yusuf Malik,Kauser A
author_facet	Cheema,Hafiza Masooma Naseer Bashir,Aftab Khatoon,Asia Iqbal,Nadia Zafar,Yusuf Malik,Kauser A
author_sort	Cheema,Hafiza Masooma Naseer
title	Molecular characterization and transcriptome profiling of expansin genes isolated from Calotropis procera fibers
title_short	Molecular characterization and transcriptome profiling of expansin genes isolated from Calotropis procera fibers
title_full	Molecular characterization and transcriptome profiling of expansin genes isolated from Calotropis procera fibers
title_fullStr	Molecular characterization and transcriptome profiling of expansin genes isolated from Calotropis procera fibers
title_full_unstemmed	Molecular characterization and transcriptome profiling of expansin genes isolated from Calotropis procera fibers
title_sort	molecular characterization and transcriptome profiling of expansin genes isolated from calotropis procera fibers
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600010
work_keys_str_mv	AT cheemahafizamasoomanaseer molecularcharacterizationandtranscriptomeprofilingofexpansingenesisolatedfromcalotropisprocerafibers AT bashiraftab molecularcharacterizationandtranscriptomeprofilingofexpansingenesisolatedfromcalotropisprocerafibers AT khatoonasia molecularcharacterizationandtranscriptomeprofilingofexpansingenesisolatedfromcalotropisprocerafibers AT iqbalnadia molecularcharacterizationandtranscriptomeprofilingofexpansingenesisolatedfromcalotropisprocerafibers AT zafaryusuf molecularcharacterizationandtranscriptomeprofilingofexpansingenesisolatedfromcalotropisprocerafibers AT malikkausera molecularcharacterizationandtranscriptomeprofilingofexpansingenesisolatedfromcalotropisprocerafibers
_version_	1718441828089331712

Molecular characterization and transcriptome profiling of expansin genes isolated from Calotropis procera fibers

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