Phenolic antioxidants extraction from raspberry wastes assisted by-enzymes

The effect of enzymatic hydrolysis on phenolic antioxidant extraction was studied in raspberry solid wastes. This by-product possesses high content of crude fiber (60%) and low values of protein, oil and ash. Raspberry fiber composition suggests that biocatalysts with cellulase, hemicellulase and pe...

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Autores principales:	Laroze,Liza, Soto,Carmen, Zúñiga,María Elvira
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2010
Materias:	antioxidant activity enzymatic extraction phenolic compounds
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600011
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spelling	oai:scielo:S0717-345820100006000112011-05-26Phenolic antioxidants extraction from raspberry wastes assisted by-enzymesLaroze,LizaSoto,CarmenZúñiga,María Elvira antioxidant activity enzymatic extraction phenolic compounds The effect of enzymatic hydrolysis on phenolic antioxidant extraction was studied in raspberry solid wastes. This by-product possesses high content of crude fiber (60%) and low values of protein, oil and ash. Raspberry fiber composition suggests that biocatalysts with cellulase, hemicellulase and pectinase activities would be useful for carrying out an enzymatically assisted antioxidant extraction. Hydro-alcoholic extraction was done using different commercial enzymes. Total phenol content and antioxidant activity of enzyme-hydrolyzed residue extracts were measured and compared with those obtained without enzyme application.All biocatalysts evaluated increased soluble solids in comparison to the non-enzymatic control. Among them, Grindamyl and Maxoliva offered the best recovery of polyphenols. Enzymatic assisted extraction with an hydro-ethanolic mixture (75:25, v/v) during 18 hrs at 50ï¿½C increased phenolic content up to 35% and antioxidant capacity around 50%, 15% and 30% according to 2,2-diphenyl-1-picrylhydrazyl = 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl) (DPPH), 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and ferric reducing antioxidant power (FRAP) methods, respectively. Use of a higher enzyme concentration significantly improved extraction of phenolic antioxidants.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.13 n.6 20102010-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600011en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	antioxidant activity enzymatic extraction phenolic compounds
spellingShingle	antioxidant activity enzymatic extraction phenolic compounds Laroze,Liza Soto,Carmen Zúñiga,María Elvira Phenolic antioxidants extraction from raspberry wastes assisted by-enzymes
description	The effect of enzymatic hydrolysis on phenolic antioxidant extraction was studied in raspberry solid wastes. This by-product possesses high content of crude fiber (60%) and low values of protein, oil and ash. Raspberry fiber composition suggests that biocatalysts with cellulase, hemicellulase and pectinase activities would be useful for carrying out an enzymatically assisted antioxidant extraction. Hydro-alcoholic extraction was done using different commercial enzymes. Total phenol content and antioxidant activity of enzyme-hydrolyzed residue extracts were measured and compared with those obtained without enzyme application.All biocatalysts evaluated increased soluble solids in comparison to the non-enzymatic control. Among them, Grindamyl and Maxoliva offered the best recovery of polyphenols. Enzymatic assisted extraction with an hydro-ethanolic mixture (75:25, v/v) during 18 hrs at 50ï¿½C increased phenolic content up to 35% and antioxidant capacity around 50%, 15% and 30% according to 2,2-diphenyl-1-picrylhydrazyl = 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl) (DPPH), 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and ferric reducing antioxidant power (FRAP) methods, respectively. Use of a higher enzyme concentration significantly improved extraction of phenolic antioxidants.
author	Laroze,Liza Soto,Carmen Zúñiga,María Elvira
author_facet	Laroze,Liza Soto,Carmen Zúñiga,María Elvira
author_sort	Laroze,Liza
title	Phenolic antioxidants extraction from raspberry wastes assisted by-enzymes
title_short	Phenolic antioxidants extraction from raspberry wastes assisted by-enzymes
title_full	Phenolic antioxidants extraction from raspberry wastes assisted by-enzymes
title_fullStr	Phenolic antioxidants extraction from raspberry wastes assisted by-enzymes
title_full_unstemmed	Phenolic antioxidants extraction from raspberry wastes assisted by-enzymes
title_sort	phenolic antioxidants extraction from raspberry wastes assisted by-enzymes
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600011
work_keys_str_mv	AT larozeliza phenolicantioxidantsextractionfromraspberrywastesassistedbyenzymes AT sotocarmen phenolicantioxidantsextractionfromraspberrywastesassistedbyenzymes AT zunigamariaelvira phenolicantioxidantsextractionfromraspberrywastesassistedbyenzymes
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Phenolic antioxidants extraction from raspberry wastes assisted by-enzymes

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