Callus induction and plant regeneration of the endemic Astragalus nezaketae in Turkey

A callus induction and plant regeneration protocol was developed from leaf and petiole explants of the endemic Astragalus nezaketae. Explants were cultured on Murashige and Skoog medium (MS) supplemented with different plant growth regulators (PGRs) [?-naphthaleneacetic acid (NAA), benzyladenine (BA...

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Autores principales:	Erisen,Semiha, Yorgancilar,Mustafa, Atalay,Emine, Babaoglu,Mehmet, Duran,Ahmet
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2010
Materias:	astragalus callus organogenesis plant regeneration
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600013
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spelling	oai:scielo:S0717-345820100006000132011-05-26Callus induction and plant regeneration of the endemic Astragalus nezaketae in TurkeyErisen,SemihaYorgancilar,MustafaAtalay,EmineBabaoglu,MehmetDuran,Ahmet astragalus callus organogenesis plant regeneration A callus induction and plant regeneration protocol was developed from leaf and petiole explants of the endemic Astragalus nezaketae. Explants were cultured on Murashige and Skoog medium (MS) supplemented with different plant growth regulators (PGRs) [?-naphthaleneacetic acid (NAA), benzyladenine (BA), 2,4-dichlorophenoxyacetic acid (2,4-D), kinetin (Kin), thidiazuron (TDZ)]. The combinations and concentrations of PGRs were shown significant variations for the frequency of callus formation, appearence of callus and the potential of callus differentiation. NAA x BA have been found highly affective in callusing and plant regeneration. Other PGRs have not resulted in callus differentiation for shoot formation. The highest number of shoots (6/explants) was obtained from leaf explants cultured on MS with 0.5 mg/l NAA and 4 mg/l BA. The regenerated shoots transferred to rooting medium (MS with 0.5 mg/l indole-3-butyric acid) were successfully rooted (100%) and showed rapid elongation. Rooted plantlets were acclimatized in pots containing 1:1 mixture of peat and perlite.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.13 n.6 20102010-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600013en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	astragalus callus organogenesis plant regeneration
spellingShingle	astragalus callus organogenesis plant regeneration Erisen,Semiha Yorgancilar,Mustafa Atalay,Emine Babaoglu,Mehmet Duran,Ahmet Callus induction and plant regeneration of the endemic Astragalus nezaketae in Turkey
description	A callus induction and plant regeneration protocol was developed from leaf and petiole explants of the endemic Astragalus nezaketae. Explants were cultured on Murashige and Skoog medium (MS) supplemented with different plant growth regulators (PGRs) [?-naphthaleneacetic acid (NAA), benzyladenine (BA), 2,4-dichlorophenoxyacetic acid (2,4-D), kinetin (Kin), thidiazuron (TDZ)]. The combinations and concentrations of PGRs were shown significant variations for the frequency of callus formation, appearence of callus and the potential of callus differentiation. NAA x BA have been found highly affective in callusing and plant regeneration. Other PGRs have not resulted in callus differentiation for shoot formation. The highest number of shoots (6/explants) was obtained from leaf explants cultured on MS with 0.5 mg/l NAA and 4 mg/l BA. The regenerated shoots transferred to rooting medium (MS with 0.5 mg/l indole-3-butyric acid) were successfully rooted (100%) and showed rapid elongation. Rooted plantlets were acclimatized in pots containing 1:1 mixture of peat and perlite.
author	Erisen,Semiha Yorgancilar,Mustafa Atalay,Emine Babaoglu,Mehmet Duran,Ahmet
author_facet	Erisen,Semiha Yorgancilar,Mustafa Atalay,Emine Babaoglu,Mehmet Duran,Ahmet
author_sort	Erisen,Semiha
title	Callus induction and plant regeneration of the endemic Astragalus nezaketae in Turkey
title_short	Callus induction and plant regeneration of the endemic Astragalus nezaketae in Turkey
title_full	Callus induction and plant regeneration of the endemic Astragalus nezaketae in Turkey
title_fullStr	Callus induction and plant regeneration of the endemic Astragalus nezaketae in Turkey
title_full_unstemmed	Callus induction and plant regeneration of the endemic Astragalus nezaketae in Turkey
title_sort	callus induction and plant regeneration of the endemic astragalus nezaketae in turkey
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600013
work_keys_str_mv	AT erisensemiha callusinductionandplantregenerationoftheendemicastragalusnezaketaeinturkey AT yorgancilarmustafa callusinductionandplantregenerationoftheendemicastragalusnezaketaeinturkey AT atalayemine callusinductionandplantregenerationoftheendemicastragalusnezaketaeinturkey AT babaoglumehmet callusinductionandplantregenerationoftheendemicastragalusnezaketaeinturkey AT duranahmet callusinductionandplantregenerationoftheendemicastragalusnezaketaeinturkey
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Callus induction and plant regeneration of the endemic Astragalus nezaketae in Turkey

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