Assessment of genetic diversity and relationships among Osmanthus fragrans cultivars using AFLP markers

This study was conducted to reveal genetic diversity among 100 Osmanthus fragrans cultivars using amplified fragment length polymorphism (AFLP) markers. Eight AFLP primer combinations produced a total of 443 polymorphic fragments with an average of 64 per primer combination. The percentage of polymo...

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Autores principales:	Yuan,Wang Jun, Han,Yuan Ji, Dong,Mei Fang, Shang,Fu De
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2011
Materias:	AFLP marker cluster analysis genetic diversity Osmanthus fragrans
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100002
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spelling	oai:scielo:S0717-345820110001000022011-05-24Assessment of genetic diversity and relationships among Osmanthus fragrans cultivars using AFLP markersYuan,Wang JunHan,Yuan JiDong,Mei FangShang,Fu De AFLP marker cluster analysis genetic diversity Osmanthus fragrans This study was conducted to reveal genetic diversity among 100 Osmanthus fragrans cultivars using amplified fragment length polymorphism (AFLP) markers. Eight AFLP primer combinations produced a total of 443 polymorphic fragments with an average of 64 per primer combination. The percentage of polymorphic bands (86.81%), the resolving power (Rp) (32.71) and the PIC values (0.331) showed the efficiency of used primer combinations. The revealed AFLP makers were effective in distinguishing all the cultivars considered. Cluster analysis were performed to assess patterns of diversity among cultivars and showed the abundant genetic diversity. The overall distribution pattern of molecular variation suggested that 93.33% of the total genetic variance was within the identified groups and 6.67% of the genetic variation was among the identified groups. Our results showed that AFLP markers are useful for Osmanthus fragrans germplasm discrimination as well as for investigation of genetic diversity and variation. The information will facilitate germplasm identiﬁcation, conservation and new cultivar development.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.14 n.1 20112011-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100002en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	AFLP marker cluster analysis genetic diversity Osmanthus fragrans
spellingShingle	AFLP marker cluster analysis genetic diversity Osmanthus fragrans Yuan,Wang Jun Han,Yuan Ji Dong,Mei Fang Shang,Fu De Assessment of genetic diversity and relationships among Osmanthus fragrans cultivars using AFLP markers
description	This study was conducted to reveal genetic diversity among 100 Osmanthus fragrans cultivars using amplified fragment length polymorphism (AFLP) markers. Eight AFLP primer combinations produced a total of 443 polymorphic fragments with an average of 64 per primer combination. The percentage of polymorphic bands (86.81%), the resolving power (Rp) (32.71) and the PIC values (0.331) showed the efficiency of used primer combinations. The revealed AFLP makers were effective in distinguishing all the cultivars considered. Cluster analysis were performed to assess patterns of diversity among cultivars and showed the abundant genetic diversity. The overall distribution pattern of molecular variation suggested that 93.33% of the total genetic variance was within the identified groups and 6.67% of the genetic variation was among the identified groups. Our results showed that AFLP markers are useful for Osmanthus fragrans germplasm discrimination as well as for investigation of genetic diversity and variation. The information will facilitate germplasm identiﬁcation, conservation and new cultivar development.
author	Yuan,Wang Jun Han,Yuan Ji Dong,Mei Fang Shang,Fu De
author_facet	Yuan,Wang Jun Han,Yuan Ji Dong,Mei Fang Shang,Fu De
author_sort	Yuan,Wang Jun
title	Assessment of genetic diversity and relationships among Osmanthus fragrans cultivars using AFLP markers
title_short	Assessment of genetic diversity and relationships among Osmanthus fragrans cultivars using AFLP markers
title_full	Assessment of genetic diversity and relationships among Osmanthus fragrans cultivars using AFLP markers
title_fullStr	Assessment of genetic diversity and relationships among Osmanthus fragrans cultivars using AFLP markers
title_full_unstemmed	Assessment of genetic diversity and relationships among Osmanthus fragrans cultivars using AFLP markers
title_sort	assessment of genetic diversity and relationships among osmanthus fragrans cultivars using aflp markers
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100002
work_keys_str_mv	AT yuanwangjun assessmentofgeneticdiversityandrelationshipsamongosmanthusfragranscultivarsusingaflpmarkers AT hanyuanji assessmentofgeneticdiversityandrelationshipsamongosmanthusfragranscultivarsusingaflpmarkers AT dongmeifang assessmentofgeneticdiversityandrelationshipsamongosmanthusfragranscultivarsusingaflpmarkers AT shangfude assessmentofgeneticdiversityandrelationshipsamongosmanthusfragranscultivarsusingaflpmarkers
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Assessment of genetic diversity and relationships among Osmanthus fragrans cultivars using AFLP markers

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