Elimination of TSWV from Impatiens hawkerii Bull. and regeneration of virus-free plant

The possibility for obtaining virus free plants from Impatiens hawkerii Bull. shoots infected with Tomato spotted wilt virus (TSWV) through meristem-tip culture was examined. TSWV presence in I. hawkerii plants was detected by DAS-ELISA and RT-PCR and identification of the virus was confirmed by seq...

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Autores principales:	Milosevic,Snezana, Subotic,Angelina, Bulajic,Aleksandra, Djekic,Ivana, Jevremovic,Sladjana, Vucurovic,Ana, Krstic,Branka
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2011
Materias:	meristem culture New Guinea Impatiens Tomato spotted wilt virus virus- free plants
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100003
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spelling	oai:scielo:S0717-345820110001000032011-05-24Elimination of TSWV from Impatiens hawkerii Bull. and regeneration of virus-free plantMilosevic,SnezanaSubotic,AngelinaBulajic,AleksandraDjekic,IvanaJevremovic,SladjanaVucurovic,AnaKrstic,Branka meristem culture New Guinea Impatiens Tomato spotted wilt virus virus- free plants The possibility for obtaining virus free plants from Impatiens hawkerii Bull. shoots infected with Tomato spotted wilt virus (TSWV) through meristem-tip culture was examined. TSWV presence in I. hawkerii plants was detected by DAS-ELISA and RT-PCR and identification of the virus was confirmed by sequencing one of the chosen isolate (GenBank Accesion CQ132190). Meristem-tip explants (0.3-1.5 mm) from virus-infected shoots are cultured on MS media supplemented with different concentrations of the cytokinins, CPPU or TDZ (0.01-1.0 µM), respectively. Using this system, a large number of in vitro shoots could be produced from a single explant. Also, cytokinins showed a stimulatory effect on the length, fresh and dry weights of the newly formed shoots. Plant pigments content in I. hawkerii shoots increased significantly in the presence of cytokinins. Rooting of shoots was spontaneous on the same media. Rooted plantlets were transferred to soil where 97% successfully acclimatized. By DAS-ELISA and RT-PCR, 80% of the in vitro plantlets were shown to be a virus-free. Considering these, the present protocol seems to be an efficient method for in vitro generation of virus-free I. hawkerii plantlets by meristem tip cultures.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.14 n.1 20112011-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100003en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	meristem culture New Guinea Impatiens Tomato spotted wilt virus virus- free plants
spellingShingle	meristem culture New Guinea Impatiens Tomato spotted wilt virus virus- free plants Milosevic,Snezana Subotic,Angelina Bulajic,Aleksandra Djekic,Ivana Jevremovic,Sladjana Vucurovic,Ana Krstic,Branka Elimination of TSWV from Impatiens hawkerii Bull. and regeneration of virus-free plant
description	The possibility for obtaining virus free plants from Impatiens hawkerii Bull. shoots infected with Tomato spotted wilt virus (TSWV) through meristem-tip culture was examined. TSWV presence in I. hawkerii plants was detected by DAS-ELISA and RT-PCR and identification of the virus was confirmed by sequencing one of the chosen isolate (GenBank Accesion CQ132190). Meristem-tip explants (0.3-1.5 mm) from virus-infected shoots are cultured on MS media supplemented with different concentrations of the cytokinins, CPPU or TDZ (0.01-1.0 µM), respectively. Using this system, a large number of in vitro shoots could be produced from a single explant. Also, cytokinins showed a stimulatory effect on the length, fresh and dry weights of the newly formed shoots. Plant pigments content in I. hawkerii shoots increased significantly in the presence of cytokinins. Rooting of shoots was spontaneous on the same media. Rooted plantlets were transferred to soil where 97% successfully acclimatized. By DAS-ELISA and RT-PCR, 80% of the in vitro plantlets were shown to be a virus-free. Considering these, the present protocol seems to be an efficient method for in vitro generation of virus-free I. hawkerii plantlets by meristem tip cultures.
author	Milosevic,Snezana Subotic,Angelina Bulajic,Aleksandra Djekic,Ivana Jevremovic,Sladjana Vucurovic,Ana Krstic,Branka
author_facet	Milosevic,Snezana Subotic,Angelina Bulajic,Aleksandra Djekic,Ivana Jevremovic,Sladjana Vucurovic,Ana Krstic,Branka
author_sort	Milosevic,Snezana
title	Elimination of TSWV from Impatiens hawkerii Bull. and regeneration of virus-free plant
title_short	Elimination of TSWV from Impatiens hawkerii Bull. and regeneration of virus-free plant
title_full	Elimination of TSWV from Impatiens hawkerii Bull. and regeneration of virus-free plant
title_fullStr	Elimination of TSWV from Impatiens hawkerii Bull. and regeneration of virus-free plant
title_full_unstemmed	Elimination of TSWV from Impatiens hawkerii Bull. and regeneration of virus-free plant
title_sort	elimination of tswv from impatiens hawkerii bull. and regeneration of virus-free plant
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100003
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Elimination of TSWV from Impatiens hawkerii Bull. and regeneration of virus-free plant

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