A real-time PCR genotyping assay to detect FAD2A SNPs in peanuts (Arachis hypogaea L.)

The high oleic (C18:1) phenotype in peanuts has been previously demonstrated to result from a homozygous recessive genotype (ol1ol1ol2ol2) in two homeologous fatty acid desaturase genes (FAD2A and FAD2B) with two key SNPs. These mutant SNPs, specifically G448A in FAD2A and 442insA in FAD2B, signific...

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Autores principales:	Barkley,Noelle A, Wang,Ming Li, Pittman,Roy N
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2011
Materias:	fatty acid composition gas chromatography peanut (Arachis hypogaea L.) real-time PCR SNP genotyping
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100009
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spelling	oai:scielo:S0717-345820110001000092011-05-24A real-time PCR genotyping assay to detect FAD2A SNPs in peanuts (Arachis hypogaea L.)Barkley,Noelle AWang,Ming LiPittman,Roy N fatty acid composition gas chromatography peanut (Arachis hypogaea L.) real-time PCR SNP genotyping The high oleic (C18:1) phenotype in peanuts has been previously demonstrated to result from a homozygous recessive genotype (ol1ol1ol2ol2) in two homeologous fatty acid desaturase genes (FAD2A and FAD2B) with two key SNPs. These mutant SNPs, specifically G448A in FAD2A and 442insA in FAD2B, significantly limit the normal function of the desaturase enzyme activity which converts oleic acid into linoleic acid by the addition of a second double bond in the hydrocarbon chain. Previously, a genotyping assay was developed to detect wild type and mutant alleles in FAD2B. A real-time PCR assay has now been developed to detect wild type and mutant alleles (G448A) in FAD2A using either seed or leaf tissue. This assay was demonstrated to be applicable for the detection of homozygous and heterozygous samples. The FAD2A genotyping assay was validated by employing gas chromatography (GC) to determine total fatty acid composition and by genotyping peanut lines that have been well characterized. Overall, development of rapid assays such as real-time PCR which can identify key genotypes associated with important agronomic traits such as oleic acid, will improve breeding efficiency by targeting desirable genotypes at early stages of development.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.14 n.1 20112011-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100009en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	fatty acid composition gas chromatography peanut (Arachis hypogaea L.) real-time PCR SNP genotyping
spellingShingle	fatty acid composition gas chromatography peanut (Arachis hypogaea L.) real-time PCR SNP genotyping Barkley,Noelle A Wang,Ming Li Pittman,Roy N A real-time PCR genotyping assay to detect FAD2A SNPs in peanuts (Arachis hypogaea L.)
description	The high oleic (C18:1) phenotype in peanuts has been previously demonstrated to result from a homozygous recessive genotype (ol1ol1ol2ol2) in two homeologous fatty acid desaturase genes (FAD2A and FAD2B) with two key SNPs. These mutant SNPs, specifically G448A in FAD2A and 442insA in FAD2B, significantly limit the normal function of the desaturase enzyme activity which converts oleic acid into linoleic acid by the addition of a second double bond in the hydrocarbon chain. Previously, a genotyping assay was developed to detect wild type and mutant alleles in FAD2B. A real-time PCR assay has now been developed to detect wild type and mutant alleles (G448A) in FAD2A using either seed or leaf tissue. This assay was demonstrated to be applicable for the detection of homozygous and heterozygous samples. The FAD2A genotyping assay was validated by employing gas chromatography (GC) to determine total fatty acid composition and by genotyping peanut lines that have been well characterized. Overall, development of rapid assays such as real-time PCR which can identify key genotypes associated with important agronomic traits such as oleic acid, will improve breeding efficiency by targeting desirable genotypes at early stages of development.
author	Barkley,Noelle A Wang,Ming Li Pittman,Roy N
author_facet	Barkley,Noelle A Wang,Ming Li Pittman,Roy N
author_sort	Barkley,Noelle A
title	A real-time PCR genotyping assay to detect FAD2A SNPs in peanuts (Arachis hypogaea L.)
title_short	A real-time PCR genotyping assay to detect FAD2A SNPs in peanuts (Arachis hypogaea L.)
title_full	A real-time PCR genotyping assay to detect FAD2A SNPs in peanuts (Arachis hypogaea L.)
title_fullStr	A real-time PCR genotyping assay to detect FAD2A SNPs in peanuts (Arachis hypogaea L.)
title_full_unstemmed	A real-time PCR genotyping assay to detect FAD2A SNPs in peanuts (Arachis hypogaea L.)
title_sort	real-time pcr genotyping assay to detect fad2a snps in peanuts (arachis hypogaea l.)
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100009
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A real-time PCR genotyping assay to detect FAD2A SNPs in peanuts (Arachis hypogaea L.)

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