Infectious pancreatic necrosis virus (IPNV) enumeration through epifluorescence microscopy: technical aspects

A method for counting Infectious pancreatic necrosis virus (IPNV) through epifluorescence microscopy was analyzed in detail. Image processing and statistic considerations are included. The particle size of viruses was compared in different experimental conditions such as the staining of the virus wi...

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Autores principales:	Pavlov,Maria Soledad, Eissler,Yoanna, Espinoza,Juan Carlos, Garland,David E, Kuznar,Juan
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2011
Materias:	digital image analysis fluorescence microscope methods immunofluorescence Infectious pancreatic necrosis virus (IPNV) SYBR-Green I
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100011
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spelling	oai:scielo:S0717-345820110001000112011-05-24Infectious pancreatic necrosis virus (IPNV) enumeration through epifluorescence microscopy: technical aspectsPavlov,Maria SoledadEissler,YoannaEspinoza,Juan CarlosGarland,David EKuznar,Juan digital image analysis fluorescence microscope methods immunofluorescence Infectious pancreatic necrosis virus (IPNV) SYBR-Green I A method for counting Infectious pancreatic necrosis virus (IPNV) through epifluorescence microscopy was analyzed in detail. Image processing and statistic considerations are included. The particle size of viruses was compared in different experimental conditions such as the staining of the virus with SYBR-Green I or with antibodies for specific fluorescence labeling of viral proteins. The type of surface used as mounting support was assayed as well. The results indicated that the most suitable method involves the mounting of the viral-containing suspension on a membrane filter followed by the staining with a monoclonal antibody specific for a viral protein combined with a FITC (fluorescein isothiocyanate)-conjugated secondary antibody.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.14 n.1 20112011-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100011en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	digital image analysis fluorescence microscope methods immunofluorescence Infectious pancreatic necrosis virus (IPNV) SYBR-Green I
spellingShingle	digital image analysis fluorescence microscope methods immunofluorescence Infectious pancreatic necrosis virus (IPNV) SYBR-Green I Pavlov,Maria Soledad Eissler,Yoanna Espinoza,Juan Carlos Garland,David E Kuznar,Juan Infectious pancreatic necrosis virus (IPNV) enumeration through epifluorescence microscopy: technical aspects
description	A method for counting Infectious pancreatic necrosis virus (IPNV) through epifluorescence microscopy was analyzed in detail. Image processing and statistic considerations are included. The particle size of viruses was compared in different experimental conditions such as the staining of the virus with SYBR-Green I or with antibodies for specific fluorescence labeling of viral proteins. The type of surface used as mounting support was assayed as well. The results indicated that the most suitable method involves the mounting of the viral-containing suspension on a membrane filter followed by the staining with a monoclonal antibody specific for a viral protein combined with a FITC (fluorescein isothiocyanate)-conjugated secondary antibody.
author	Pavlov,Maria Soledad Eissler,Yoanna Espinoza,Juan Carlos Garland,David E Kuznar,Juan
author_facet	Pavlov,Maria Soledad Eissler,Yoanna Espinoza,Juan Carlos Garland,David E Kuznar,Juan
author_sort	Pavlov,Maria Soledad
title	Infectious pancreatic necrosis virus (IPNV) enumeration through epifluorescence microscopy: technical aspects
title_short	Infectious pancreatic necrosis virus (IPNV) enumeration through epifluorescence microscopy: technical aspects
title_full	Infectious pancreatic necrosis virus (IPNV) enumeration through epifluorescence microscopy: technical aspects
title_fullStr	Infectious pancreatic necrosis virus (IPNV) enumeration through epifluorescence microscopy: technical aspects
title_full_unstemmed	Infectious pancreatic necrosis virus (IPNV) enumeration through epifluorescence microscopy: technical aspects
title_sort	infectious pancreatic necrosis virus (ipnv) enumeration through epifluorescence microscopy: technical aspects
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100011
work_keys_str_mv	AT pavlovmariasoledad infectiouspancreaticnecrosisvirusipnvenumerationthroughepifluorescencemicroscopytechnicalaspects AT eissleryoanna infectiouspancreaticnecrosisvirusipnvenumerationthroughepifluorescencemicroscopytechnicalaspects AT espinozajuancarlos infectiouspancreaticnecrosisvirusipnvenumerationthroughepifluorescencemicroscopytechnicalaspects AT garlanddavide infectiouspancreaticnecrosisvirusipnvenumerationthroughepifluorescencemicroscopytechnicalaspects AT kuznarjuan infectiouspancreaticnecrosisvirusipnvenumerationthroughepifluorescencemicroscopytechnicalaspects
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Infectious pancreatic necrosis virus (IPNV) enumeration through epifluorescence microscopy: technical aspects

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