Characterization of novel genic SSR markers in Linum usitatissimum (L.) and their transferability across eleven Linum species

Little is known about the evolutionary relationships among Linum species, basically because of the lack of transferable molecular markers. Currently, expressed sequence tags available in public databases provide an opportunity for the rapid and inexpensive development of simple sequence repeat (SSR)...

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Autores principales:	Soto-Cerda,Braulio J, Saavedra,Hector Urbina, Navarro,Cristell Navarro, Ortega,Paula Mora
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2011
Materias:	EST-SSRs expressed sequence tag flaxseed polymorphic loci relatives
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000200004
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spelling	oai:scielo:S0717-345820110002000042011-05-26Characterization of novel genic SSR markers in Linum usitatissimum (L.) and their transferability across eleven Linum speciesSoto-Cerda,Braulio JSaavedra,Hector UrbinaNavarro,Cristell NavarroOrtega,Paula Mora EST-SSRs expressed sequence tag flaxseed polymorphic loci relatives Little is known about the evolutionary relationships among Linum species, basically because of the lack of transferable molecular markers. Currently, expressed sequence tags available in public databases provide an opportunity for the rapid and inexpensive development of simple sequence repeat (SSR) markers in wild flax species. In this regard, fifty expressed sequence tag-derived microsatellite markers (EST-SSRs) were evaluated for polymorphism and transferability in 50 Linum usitatissimum cultivars/accessions and 11 Linum species. Among them 23 EST-SSRs were polymorphic in L. usitatissimum, while 2-4 alleles were detected (average 2.26 per locus). The polymorphism information content value ranged from 0.08 to 0.55 (average 0.38). Forty one genic markers (95.3%) produced strong amplicons in at least two of the 11 Linum species. The percentage of cross amplification ranged from 34.1% to 92.7% in L. tauricum and L. bienne, respectively. Moreover, the rate of transferability was associated positively with the botanical section. Our results suggest that the high degree of EST-SSRs transferability to Linum species can be a useful enhancement of the current database of SSR markers for future genetic and evolutionary studies.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.14 n.2 20112011-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000200004en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	EST-SSRs expressed sequence tag flaxseed polymorphic loci relatives
spellingShingle	EST-SSRs expressed sequence tag flaxseed polymorphic loci relatives Soto-Cerda,Braulio J Saavedra,Hector Urbina Navarro,Cristell Navarro Ortega,Paula Mora Characterization of novel genic SSR markers in Linum usitatissimum (L.) and their transferability across eleven Linum species
description	Little is known about the evolutionary relationships among Linum species, basically because of the lack of transferable molecular markers. Currently, expressed sequence tags available in public databases provide an opportunity for the rapid and inexpensive development of simple sequence repeat (SSR) markers in wild flax species. In this regard, fifty expressed sequence tag-derived microsatellite markers (EST-SSRs) were evaluated for polymorphism and transferability in 50 Linum usitatissimum cultivars/accessions and 11 Linum species. Among them 23 EST-SSRs were polymorphic in L. usitatissimum, while 2-4 alleles were detected (average 2.26 per locus). The polymorphism information content value ranged from 0.08 to 0.55 (average 0.38). Forty one genic markers (95.3%) produced strong amplicons in at least two of the 11 Linum species. The percentage of cross amplification ranged from 34.1% to 92.7% in L. tauricum and L. bienne, respectively. Moreover, the rate of transferability was associated positively with the botanical section. Our results suggest that the high degree of EST-SSRs transferability to Linum species can be a useful enhancement of the current database of SSR markers for future genetic and evolutionary studies.
author	Soto-Cerda,Braulio J Saavedra,Hector Urbina Navarro,Cristell Navarro Ortega,Paula Mora
author_facet	Soto-Cerda,Braulio J Saavedra,Hector Urbina Navarro,Cristell Navarro Ortega,Paula Mora
author_sort	Soto-Cerda,Braulio J
title	Characterization of novel genic SSR markers in Linum usitatissimum (L.) and their transferability across eleven Linum species
title_short	Characterization of novel genic SSR markers in Linum usitatissimum (L.) and their transferability across eleven Linum species
title_full	Characterization of novel genic SSR markers in Linum usitatissimum (L.) and their transferability across eleven Linum species
title_fullStr	Characterization of novel genic SSR markers in Linum usitatissimum (L.) and their transferability across eleven Linum species
title_full_unstemmed	Characterization of novel genic SSR markers in Linum usitatissimum (L.) and their transferability across eleven Linum species
title_sort	characterization of novel genic ssr markers in linum usitatissimum (l.) and their transferability across eleven linum species
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000200004
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Characterization of novel genic SSR markers in Linum usitatissimum (L.) and their transferability across eleven Linum species

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