High density process to cultivate Lactobacillus plantarum biomass using wheat stillage and sugar beet molasses

Background: Owing to the growing interest in biofuels, the concept of a biorefinery where biomass is converted to a variety of useful products is gaining ground. We here present how distillery waste is combined with a by-product from a sugar production, molasses, to form a medium for the growth of L...

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Autores principales:	Krzywonos,Magorzata, Eberhard,Thomas
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2011
Materias:	distillery wastewater high density fermentation lactic acid bacteria Lactobacillus plantarum sugar beet molasses wheat stillage
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000200006
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spelling	oai:scielo:S0717-345820110002000062011-05-26High density process to cultivate Lactobacillus plantarum biomass using wheat stillage and sugar beet molassesKrzywonos,MagorzataEberhard,Thomas distillery wastewater high density fermentation lactic acid bacteria Lactobacillus plantarum sugar beet molasses wheat stillage Background: Owing to the growing interest in biofuels, the concept of a biorefinery where biomass is converted to a variety of useful products is gaining ground. We here present how distillery waste is combined with a by-product from a sugar production, molasses, to form a medium for the growth of Lactobacillus plantarum with yields and biomass densities comparable with conventional industrial media. Such approach enables a cost-effective utilization of the problematic wastewater from ethanol and a by-product from sugar production. It is the first approach that attempts to find low-cost media for the production of Lactobacillus plantarum biomass. Results: This study suggests that sieved wheat stillage enriched by adding 1.77 g/l yeast extract and 10% molasses (v/v), with NH4OH used for pH adjustment, may be used as a media for large-scale cultivation of L. plantarum. Such composition of the medium permits a high density of lactic acid bacteria (1.6 x 10(10) cfu/ml) to be achieved. Conclusions: The use of a fermentation medium consisting of distillery wastewater and molasses to obtain value-added products (such as LAB biomass and lactic acid) is a possible step for classical ethanol production to move towards a biorefinery model production in which all by and waste products are utilized to increase produced values and reduce waste production. This enables a cost-effective utilization of the problematic wastewater from ethanol and sugar production.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.14 n.2 20112011-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000200006en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	distillery wastewater high density fermentation lactic acid bacteria Lactobacillus plantarum sugar beet molasses wheat stillage
spellingShingle	distillery wastewater high density fermentation lactic acid bacteria Lactobacillus plantarum sugar beet molasses wheat stillage Krzywonos,Magorzata Eberhard,Thomas High density process to cultivate Lactobacillus plantarum biomass using wheat stillage and sugar beet molasses
description	Background: Owing to the growing interest in biofuels, the concept of a biorefinery where biomass is converted to a variety of useful products is gaining ground. We here present how distillery waste is combined with a by-product from a sugar production, molasses, to form a medium for the growth of Lactobacillus plantarum with yields and biomass densities comparable with conventional industrial media. Such approach enables a cost-effective utilization of the problematic wastewater from ethanol and a by-product from sugar production. It is the first approach that attempts to find low-cost media for the production of Lactobacillus plantarum biomass. Results: This study suggests that sieved wheat stillage enriched by adding 1.77 g/l yeast extract and 10% molasses (v/v), with NH4OH used for pH adjustment, may be used as a media for large-scale cultivation of L. plantarum. Such composition of the medium permits a high density of lactic acid bacteria (1.6 x 10(10) cfu/ml) to be achieved. Conclusions: The use of a fermentation medium consisting of distillery wastewater and molasses to obtain value-added products (such as LAB biomass and lactic acid) is a possible step for classical ethanol production to move towards a biorefinery model production in which all by and waste products are utilized to increase produced values and reduce waste production. This enables a cost-effective utilization of the problematic wastewater from ethanol and sugar production.
author	Krzywonos,Magorzata Eberhard,Thomas
author_facet	Krzywonos,Magorzata Eberhard,Thomas
author_sort	Krzywonos,Magorzata
title	High density process to cultivate Lactobacillus plantarum biomass using wheat stillage and sugar beet molasses
title_short	High density process to cultivate Lactobacillus plantarum biomass using wheat stillage and sugar beet molasses
title_full	High density process to cultivate Lactobacillus plantarum biomass using wheat stillage and sugar beet molasses
title_fullStr	High density process to cultivate Lactobacillus plantarum biomass using wheat stillage and sugar beet molasses
title_full_unstemmed	High density process to cultivate Lactobacillus plantarum biomass using wheat stillage and sugar beet molasses
title_sort	high density process to cultivate lactobacillus plantarum biomass using wheat stillage and sugar beet molasses
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000200006
work_keys_str_mv	AT krzywonosmagorzata highdensityprocesstocultivatelactobacillusplantarumbiomassusingwheatstillageandsugarbeetmolasses AT eberhardthomas highdensityprocesstocultivatelactobacillusplantarumbiomassusingwheatstillageandsugarbeetmolasses
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High density process to cultivate Lactobacillus plantarum biomass using wheat stillage and sugar beet molasses

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