Xylanolytic enzymes production by Aspergillus niger GS1 from solid-state fermentation on corn stover and their effect on ruminal digestibility

Hemicellulosic agricultural by-products such as corn stover (CS) are highly available materials which represent an opportunity to develop value added products. Native Aspergillus niger GS1 was used for solid-state fermentation (SSF) on alkali pre-treated CS (ACS) aimed to optimize xylanolytic enzyme...

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Autores principales:	Regalado,Carlos, Vázquez-Obregón,Immer, García-Almendárez,Blanca Estela, Domínguez-Domínguez,Jorge, Aguilera-Barreyro,Araceli, Amaro-Reyes,Aldo
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2011
Materias:	Aspergillus niger GS1 corn stover digestibility solid-state fermentation xylanolytic activity
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000200009
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spelling	oai:scielo:S0717-345820110002000092011-05-26Xylanolytic enzymes production by Aspergillus niger GS1 from solid-state fermentation on corn stover and their effect on ruminal digestibilityRegalado,CarlosVázquez-Obregón,ImmerGarcía-Almendárez,Blanca EstelaDomínguez-Domínguez,JorgeAguilera-Barreyro,AraceliAmaro-Reyes,Aldo Aspergillus niger GS1 corn stover digestibility solid-state fermentation xylanolytic activity Hemicellulosic agricultural by-products such as corn stover (CS) are highly available materials which represent an opportunity to develop value added products. Native Aspergillus niger GS1 was used for solid-state fermentation (SSF) on alkali pre-treated CS (ACS) aimed to optimize xylanolytic enzymes production, and their effect on in vitro ruminal and true digestibility of ACS. Enzyme production was empirically modelled using a fractional factorial design 2(9-5), and the resulting significant factors were glucose, yeast extract and two mineral salts, which were arranged in a Draper-Lin optimization design at two levels. Predicted optimum xylanolytic activity of 33.6 U (mg protein)-1 was achieved at 48 hrs of SSF, and was validated by confirmatory experiments. ACS was incubated with a semipurified enzymatic extract (EE) showing a xylanolytic activity of 1600 U kg-1 dry ACS for 12 hrs before exposure to cow?s ruminal liquid for 72 hrs, which led to 5% and 10% increase of in vitro ruminal and true digestibility, respectively. CS is a readily available by-product in different regions which after alkaline treatment and partial hydrolysis with the EE, may be advantageously used as supplement for ruminant feed.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.14 n.2 20112011-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000200009en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Aspergillus niger GS1 corn stover digestibility solid-state fermentation xylanolytic activity
spellingShingle	Aspergillus niger GS1 corn stover digestibility solid-state fermentation xylanolytic activity Regalado,Carlos Vázquez-Obregón,Immer García-Almendárez,Blanca Estela Domínguez-Domínguez,Jorge Aguilera-Barreyro,Araceli Amaro-Reyes,Aldo Xylanolytic enzymes production by Aspergillus niger GS1 from solid-state fermentation on corn stover and their effect on ruminal digestibility
description	Hemicellulosic agricultural by-products such as corn stover (CS) are highly available materials which represent an opportunity to develop value added products. Native Aspergillus niger GS1 was used for solid-state fermentation (SSF) on alkali pre-treated CS (ACS) aimed to optimize xylanolytic enzymes production, and their effect on in vitro ruminal and true digestibility of ACS. Enzyme production was empirically modelled using a fractional factorial design 2(9-5), and the resulting significant factors were glucose, yeast extract and two mineral salts, which were arranged in a Draper-Lin optimization design at two levels. Predicted optimum xylanolytic activity of 33.6 U (mg protein)-1 was achieved at 48 hrs of SSF, and was validated by confirmatory experiments. ACS was incubated with a semipurified enzymatic extract (EE) showing a xylanolytic activity of 1600 U kg-1 dry ACS for 12 hrs before exposure to cow?s ruminal liquid for 72 hrs, which led to 5% and 10% increase of in vitro ruminal and true digestibility, respectively. CS is a readily available by-product in different regions which after alkaline treatment and partial hydrolysis with the EE, may be advantageously used as supplement for ruminant feed.
author	Regalado,Carlos Vázquez-Obregón,Immer García-Almendárez,Blanca Estela Domínguez-Domínguez,Jorge Aguilera-Barreyro,Araceli Amaro-Reyes,Aldo
author_facet	Regalado,Carlos Vázquez-Obregón,Immer García-Almendárez,Blanca Estela Domínguez-Domínguez,Jorge Aguilera-Barreyro,Araceli Amaro-Reyes,Aldo
author_sort	Regalado,Carlos
title	Xylanolytic enzymes production by Aspergillus niger GS1 from solid-state fermentation on corn stover and their effect on ruminal digestibility
title_short	Xylanolytic enzymes production by Aspergillus niger GS1 from solid-state fermentation on corn stover and their effect on ruminal digestibility
title_full	Xylanolytic enzymes production by Aspergillus niger GS1 from solid-state fermentation on corn stover and their effect on ruminal digestibility
title_fullStr	Xylanolytic enzymes production by Aspergillus niger GS1 from solid-state fermentation on corn stover and their effect on ruminal digestibility
title_full_unstemmed	Xylanolytic enzymes production by Aspergillus niger GS1 from solid-state fermentation on corn stover and their effect on ruminal digestibility
title_sort	xylanolytic enzymes production by aspergillus niger gs1 from solid-state fermentation on corn stover and their effect on ruminal digestibility
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000200009
work_keys_str_mv	AT regaladocarlos xylanolyticenzymesproductionbyaspergillusnigergs1fromsolidstatefermentationoncornstoverandtheireffectonruminaldigestibility AT vazquezobregonimmer xylanolyticenzymesproductionbyaspergillusnigergs1fromsolidstatefermentationoncornstoverandtheireffectonruminaldigestibility AT garciaalmendarezblancaestela xylanolyticenzymesproductionbyaspergillusnigergs1fromsolidstatefermentationoncornstoverandtheireffectonruminaldigestibility AT dominguezdominguezjorge xylanolyticenzymesproductionbyaspergillusnigergs1fromsolidstatefermentationoncornstoverandtheireffectonruminaldigestibility AT aguilerabarreyroaraceli xylanolyticenzymesproductionbyaspergillusnigergs1fromsolidstatefermentationoncornstoverandtheireffectonruminaldigestibility AT amaroreyesaldo xylanolyticenzymesproductionbyaspergillusnigergs1fromsolidstatefermentationoncornstoverandtheireffectonruminaldigestibility
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Xylanolytic enzymes production by Aspergillus niger GS1 from solid-state fermentation on corn stover and their effect on ruminal digestibility

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