Sodium azide mutagenesis resulted in a peanut plant with elevated oleate content

Screening of peanut seeds resulting from 0.39% sodium azide treatment with NIRS calibration equation for bulk seed samples identified a plant with more than 60% oleate. Oleate content in individual seeds of the plant, as predicted by NIRS calibration equation for intact single peanut seeds, ranged f...

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Autores principales:	Wang,Chuan Tang, Tang,Yue Yi, Wang,Xiu Zhen, Zhang,Shu Wei, Li,Gui Jie, Zhang,Jian Cheng, Yu,Shan Lin
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2011
Materias:	chemical mutant FAD2B NIRS oleate peanut
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000200010
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spelling	oai:scielo:S0717-345820110002000102011-05-26Sodium azide mutagenesis resulted in a peanut plant with elevated oleate contentWang,Chuan TangTang,Yue YiWang,Xiu ZhenZhang,Shu WeiLi,Gui JieZhang,Jian ChengYu,Shan Lin chemical mutant FAD2B NIRS oleate peanut Screening of peanut seeds resulting from 0.39% sodium azide treatment with NIRS calibration equation for bulk seed samples identified a plant with more than 60% oleate. Oleate content in individual seeds of the plant, as predicted by NIRS calibration equation for intact single peanut seeds, ranged from 50.05% ~ 68.69%. Three seeds with >60% oleate thus identified were further confirmed by gas chromatography. Multiple sequence alignments of the FAD2B gene from Huayu 22 (wild type) and peanut seeds with elevated oleate (mutant type) revealed a C281T transition in the coding region causing an I94T substitution in the oleoyl-PC desaturase, which may be responsible for reduction in the enzyme activity.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.14 n.2 20112011-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000200010en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	chemical mutant FAD2B NIRS oleate peanut
spellingShingle	chemical mutant FAD2B NIRS oleate peanut Wang,Chuan Tang Tang,Yue Yi Wang,Xiu Zhen Zhang,Shu Wei Li,Gui Jie Zhang,Jian Cheng Yu,Shan Lin Sodium azide mutagenesis resulted in a peanut plant with elevated oleate content
description	Screening of peanut seeds resulting from 0.39% sodium azide treatment with NIRS calibration equation for bulk seed samples identified a plant with more than 60% oleate. Oleate content in individual seeds of the plant, as predicted by NIRS calibration equation for intact single peanut seeds, ranged from 50.05% ~ 68.69%. Three seeds with >60% oleate thus identified were further confirmed by gas chromatography. Multiple sequence alignments of the FAD2B gene from Huayu 22 (wild type) and peanut seeds with elevated oleate (mutant type) revealed a C281T transition in the coding region causing an I94T substitution in the oleoyl-PC desaturase, which may be responsible for reduction in the enzyme activity.
author	Wang,Chuan Tang Tang,Yue Yi Wang,Xiu Zhen Zhang,Shu Wei Li,Gui Jie Zhang,Jian Cheng Yu,Shan Lin
author_facet	Wang,Chuan Tang Tang,Yue Yi Wang,Xiu Zhen Zhang,Shu Wei Li,Gui Jie Zhang,Jian Cheng Yu,Shan Lin
author_sort	Wang,Chuan Tang
title	Sodium azide mutagenesis resulted in a peanut plant with elevated oleate content
title_short	Sodium azide mutagenesis resulted in a peanut plant with elevated oleate content
title_full	Sodium azide mutagenesis resulted in a peanut plant with elevated oleate content
title_fullStr	Sodium azide mutagenesis resulted in a peanut plant with elevated oleate content
title_full_unstemmed	Sodium azide mutagenesis resulted in a peanut plant with elevated oleate content
title_sort	sodium azide mutagenesis resulted in a peanut plant with elevated oleate content
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000200010
work_keys_str_mv	AT wangchuantang sodiumazidemutagenesisresultedinapeanutplantwithelevatedoleatecontent AT tangyueyi sodiumazidemutagenesisresultedinapeanutplantwithelevatedoleatecontent AT wangxiuzhen sodiumazidemutagenesisresultedinapeanutplantwithelevatedoleatecontent AT zhangshuwei sodiumazidemutagenesisresultedinapeanutplantwithelevatedoleatecontent AT liguijie sodiumazidemutagenesisresultedinapeanutplantwithelevatedoleatecontent AT zhangjiancheng sodiumazidemutagenesisresultedinapeanutplantwithelevatedoleatecontent AT yushanlin sodiumazidemutagenesisresultedinapeanutplantwithelevatedoleatecontent
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Sodium azide mutagenesis resulted in a peanut plant with elevated oleate content

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