Construction and application of a built-in dual luciferase reporter for microRNA functional analysis
Background: As key gene regulators, microRNAs post-transcriptionally modulate gene expression via binding to partially complementary sequence in the 3? UTR of target mRNA. An accurate, rapid and quantitative tool for sensing and validation of miRNA targets is of crucial significance to decipher the...
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Auteurs principaux: | , , , , , |
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Langue: | English |
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Pontificia Universidad Católica de Valparaíso
2011
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Accès en ligne: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000200011 |
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