Developmental rates of bovine nuclear transfer embryos derived from different fetal non transfected and transfected cells

Since the first successful somatic cell nuclear transfer (SCNT) experiments were carried out, a number of domestic and agriculture species have been cloned using donor cells derived from different sources and origin. However, differences in nuclear transfer efficiency both in vitro and in vivo have...

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Autores principales:	Felmer D,Ricardo, Arias,María Elena
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2011
Materias:	bovine embryos fetal fibroblasts nuclear transfer transfected cells
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300005
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spelling	oai:scielo:S0717-345820110003000052011-09-23Developmental rates of bovine nuclear transfer embryos derived from different fetal non transfected and transfected cellsFelmer D,RicardoArias,María Elena bovine embryos fetal fibroblasts nuclear transfer transfected cells Since the first successful somatic cell nuclear transfer (SCNT) experiments were carried out, a number of domestic and agriculture species have been cloned using donor cells derived from different sources and origin. However, differences in nuclear transfer efficiency both in vitro and in vivo have been generally observed. These differences may be accentuated when transgenic cell lines are used as nuclear donors in an attempt to generate transgenic cloned offspring. The present study examined the suitability of cell lines derived from 3 different fetal sources and the effects of genetic manipulation of donor fetal fibroblasts with a red fluorescent plasmid, on the in vitro developmental potential and quality of nuclear transfer derived bovine embryos. We observed no differences in the cleavage rate of nuclear transfer embryos generated with any of the cell lines evaluated. However, the blastocyst rate was significantly affected when cell lines were derived from the 3 different fetal sources (21, 18 and 11%, respectively) or from 2 transgenic clonal cell lines that had originated from the same primary fetal cell (18 and 10%, respectively). Despite this difference, quality of embryos as measured by the total number of cells and by assessing some morphology aspects of their appearance was not different. Together these results indicate that fetal fibroblast cell lines derived from different fetal sources and transgenic clonal cell lines that had originated from the same fetus results in different in vitro developmental potential when used as donors for nuclear transfer experiments. Further studies, including evaluation of pregnancy rates, development to term, and epigenetic modifications of these cell lines will be necessary to better understand the differences observed in nuclear transfer efficiency.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.14 n.3 20112011-05-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300005en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	bovine embryos fetal fibroblasts nuclear transfer transfected cells
spellingShingle	bovine embryos fetal fibroblasts nuclear transfer transfected cells Felmer D,Ricardo Arias,María Elena Developmental rates of bovine nuclear transfer embryos derived from different fetal non transfected and transfected cells
description	Since the first successful somatic cell nuclear transfer (SCNT) experiments were carried out, a number of domestic and agriculture species have been cloned using donor cells derived from different sources and origin. However, differences in nuclear transfer efficiency both in vitro and in vivo have been generally observed. These differences may be accentuated when transgenic cell lines are used as nuclear donors in an attempt to generate transgenic cloned offspring. The present study examined the suitability of cell lines derived from 3 different fetal sources and the effects of genetic manipulation of donor fetal fibroblasts with a red fluorescent plasmid, on the in vitro developmental potential and quality of nuclear transfer derived bovine embryos. We observed no differences in the cleavage rate of nuclear transfer embryos generated with any of the cell lines evaluated. However, the blastocyst rate was significantly affected when cell lines were derived from the 3 different fetal sources (21, 18 and 11%, respectively) or from 2 transgenic clonal cell lines that had originated from the same primary fetal cell (18 and 10%, respectively). Despite this difference, quality of embryos as measured by the total number of cells and by assessing some morphology aspects of their appearance was not different. Together these results indicate that fetal fibroblast cell lines derived from different fetal sources and transgenic clonal cell lines that had originated from the same fetus results in different in vitro developmental potential when used as donors for nuclear transfer experiments. Further studies, including evaluation of pregnancy rates, development to term, and epigenetic modifications of these cell lines will be necessary to better understand the differences observed in nuclear transfer efficiency.
author	Felmer D,Ricardo Arias,María Elena
author_facet	Felmer D,Ricardo Arias,María Elena
author_sort	Felmer D,Ricardo
title	Developmental rates of bovine nuclear transfer embryos derived from different fetal non transfected and transfected cells
title_short	Developmental rates of bovine nuclear transfer embryos derived from different fetal non transfected and transfected cells
title_full	Developmental rates of bovine nuclear transfer embryos derived from different fetal non transfected and transfected cells
title_fullStr	Developmental rates of bovine nuclear transfer embryos derived from different fetal non transfected and transfected cells
title_full_unstemmed	Developmental rates of bovine nuclear transfer embryos derived from different fetal non transfected and transfected cells
title_sort	developmental rates of bovine nuclear transfer embryos derived from different fetal non transfected and transfected cells
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300005
work_keys_str_mv	AT felmerdricardo developmentalratesofbovinenucleartransferembryosderivedfromdifferentfetalnontransfectedandtransfectedcells AT ariasmariaelena developmentalratesofbovinenucleartransferembryosderivedfromdifferentfetalnontransfectedandtransfectedcells
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Developmental rates of bovine nuclear transfer embryos derived from different fetal non transfected and transfected cells

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