Identification of leaf rust resistance genes in selected Argentinean bread wheat cultivars by gene postulation and molecular markers

Leaf rust, caused by Puccinia triticina Eriks. is a common and widespread disease of bread wheat (Triticum aestivum L.), in Argentina. Host resistance is the most economical, effective and ecologically sustainable method of controlling the disease. Gene postulation helps to determine leaf rust resis...

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Autores principales:	Vanzetti,Leonardo Sebastián, Campos,Pablo, Demichelis,Melina, Lombardo,Lucio Andres, Aurelia,Paola Romina, Vaschetto,Luis María, Bainotti,Carlos Tomás, Helguera,Marcelo
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2011
Materias:	genetic control of pathogens molecular markers plant breeding
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300009
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spelling	oai:scielo:S0717-345820110003000092011-09-23Identification of leaf rust resistance genes in selected Argentinean bread wheat cultivars by gene postulation and molecular markersVanzetti,Leonardo SebastiánCampos,PabloDemichelis,MelinaLombardo,Lucio AndresAurelia,Paola RominaVaschetto,Luis MaríaBainotti,Carlos TomásHelguera,Marcelo genetic control of pathogens molecular markers plant breeding Leaf rust, caused by Puccinia triticina Eriks. is a common and widespread disease of bread wheat (Triticum aestivum L.), in Argentina. Host resistance is the most economical, effective and ecologically sustainable method of controlling the disease. Gene postulation helps to determine leaf rust resistance genes (Lr genes) that may be present in a large group of wheat germplasm. Additionally presence of Lr genes can be determined using associated molecular markers. The objective of this study was to identify Lr genes that condition leaf rust resistance in 66 wheat cultivars from Argentina. Twenty four differential lines with individual known leaf rust resistance genes were tested with 17 different pathotypes of leaf rust collected from Argentina. Leaf rust infection types produced on seedling plants of the 66 local cultivars were compared with the infection types produced by the same pathotypes on Lr differentials to postulate which seedling leaf rust genes were present. Presence of Lr9, Lr10, Lr19, Lr20, Lr21, Lr24, Lr25, Lr26, Lr29, Lr34, Lr35, Lr37, Lr47 and Lr51 was also determined using molecular markers. Eleven different Lr genes were postulated in the material: Lr1, Lr3a, Lr3ka, Lr9, Lr10, Lr16, Lr17, Lr19, Lr24, Lr26, Lr41. Presence of Lr21, Lr25, Lr29, and Lr47 could not be determined with the seventeen pathotypes used in the study because all were avirulent to these genes. Eleven cultivars (16.7%) were resistant to all pathotypes used in the study and the remaining 55 (83.3%) showed virulent reaction against one or more local pathotypes. Cultivars with seedling resistance gene combinations including Lr16 or single genes Lr47 (detected with molecular marker), Lr19 and Lr41, showed high levels of resistance against all pathotypes or most of them. On the opposite side, cultivars with seedling resistance genes Lr1, Lr3a, Lr3a + Lr24, Lr10, Lr3a + Lr10, Lr3a + Lr10 + Lr24 showed the highest number of virulent reactions against local pathotypes. Occurrence of adult plant resistance genes Lr34, Lr35 and Lr37 in local germplasm was evaluated using specific molecular markers confirming presence of Lr34 and Lr37. Our data suggest that combinations including seedling resistance genes like Lr16, Lr47, Lr19, Lr41, Lr21, Lr25 and Lr29, with adult plant resistance genes like Lr34, SV2, Lr46 will probably provide durable and effective resistance to leaf rust in the region.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.14 n.3 20112011-05-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300009en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	genetic control of pathogens molecular markers plant breeding
spellingShingle	genetic control of pathogens molecular markers plant breeding Vanzetti,Leonardo Sebastián Campos,Pablo Demichelis,Melina Lombardo,Lucio Andres Aurelia,Paola Romina Vaschetto,Luis María Bainotti,Carlos Tomás Helguera,Marcelo Identification of leaf rust resistance genes in selected Argentinean bread wheat cultivars by gene postulation and molecular markers
description	Leaf rust, caused by Puccinia triticina Eriks. is a common and widespread disease of bread wheat (Triticum aestivum L.), in Argentina. Host resistance is the most economical, effective and ecologically sustainable method of controlling the disease. Gene postulation helps to determine leaf rust resistance genes (Lr genes) that may be present in a large group of wheat germplasm. Additionally presence of Lr genes can be determined using associated molecular markers. The objective of this study was to identify Lr genes that condition leaf rust resistance in 66 wheat cultivars from Argentina. Twenty four differential lines with individual known leaf rust resistance genes were tested with 17 different pathotypes of leaf rust collected from Argentina. Leaf rust infection types produced on seedling plants of the 66 local cultivars were compared with the infection types produced by the same pathotypes on Lr differentials to postulate which seedling leaf rust genes were present. Presence of Lr9, Lr10, Lr19, Lr20, Lr21, Lr24, Lr25, Lr26, Lr29, Lr34, Lr35, Lr37, Lr47 and Lr51 was also determined using molecular markers. Eleven different Lr genes were postulated in the material: Lr1, Lr3a, Lr3ka, Lr9, Lr10, Lr16, Lr17, Lr19, Lr24, Lr26, Lr41. Presence of Lr21, Lr25, Lr29, and Lr47 could not be determined with the seventeen pathotypes used in the study because all were avirulent to these genes. Eleven cultivars (16.7%) were resistant to all pathotypes used in the study and the remaining 55 (83.3%) showed virulent reaction against one or more local pathotypes. Cultivars with seedling resistance gene combinations including Lr16 or single genes Lr47 (detected with molecular marker), Lr19 and Lr41, showed high levels of resistance against all pathotypes or most of them. On the opposite side, cultivars with seedling resistance genes Lr1, Lr3a, Lr3a + Lr24, Lr10, Lr3a + Lr10, Lr3a + Lr10 + Lr24 showed the highest number of virulent reactions against local pathotypes. Occurrence of adult plant resistance genes Lr34, Lr35 and Lr37 in local germplasm was evaluated using specific molecular markers confirming presence of Lr34 and Lr37. Our data suggest that combinations including seedling resistance genes like Lr16, Lr47, Lr19, Lr41, Lr21, Lr25 and Lr29, with adult plant resistance genes like Lr34, SV2, Lr46 will probably provide durable and effective resistance to leaf rust in the region.
author	Vanzetti,Leonardo Sebastián Campos,Pablo Demichelis,Melina Lombardo,Lucio Andres Aurelia,Paola Romina Vaschetto,Luis María Bainotti,Carlos Tomás Helguera,Marcelo
author_facet	Vanzetti,Leonardo Sebastián Campos,Pablo Demichelis,Melina Lombardo,Lucio Andres Aurelia,Paola Romina Vaschetto,Luis María Bainotti,Carlos Tomás Helguera,Marcelo
author_sort	Vanzetti,Leonardo Sebastián
title	Identification of leaf rust resistance genes in selected Argentinean bread wheat cultivars by gene postulation and molecular markers
title_short	Identification of leaf rust resistance genes in selected Argentinean bread wheat cultivars by gene postulation and molecular markers
title_full	Identification of leaf rust resistance genes in selected Argentinean bread wheat cultivars by gene postulation and molecular markers
title_fullStr	Identification of leaf rust resistance genes in selected Argentinean bread wheat cultivars by gene postulation and molecular markers
title_full_unstemmed	Identification of leaf rust resistance genes in selected Argentinean bread wheat cultivars by gene postulation and molecular markers
title_sort	identification of leaf rust resistance genes in selected argentinean bread wheat cultivars by gene postulation and molecular markers
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300009
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Identification of leaf rust resistance genes in selected Argentinean bread wheat cultivars by gene postulation and molecular markers

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