A preferable approach to clone hLIF cDNA from the genomic DNA
Complementary DNA (cDNA) is valuable for investigating protein structure and function in the research of life science, but it is difficult to obtain by traditional reverse transcription. In this study, we employed a novel strategy to clone the human leukemia inhibitory factor (hLIF) gene cDNA from g...
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| Lenguaje: | English |
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Pontificia Universidad Católica de Valparaíso
2011
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| Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300012 |
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oai:scielo:S0717-345820110003000122011-09-23A preferable approach to clone hLIF cDNA from the genomic DNACui,YihongZhu,GuangqinChen,QiujuWang,YunfeiYang,MingmingSong,YuxuanWang,JiangangCao,Binyun cDNA cloning hLIF oral mucous membrane overlap PCR Complementary DNA (cDNA) is valuable for investigating protein structure and function in the research of life science, but it is difficult to obtain by traditional reverse transcription. In this study, we employed a novel strategy to clone the human leukemia inhibitory factor (hLIF) gene cDNA from genomic DNA directly isolated from the mucous membrane of mouth. The hLIF sequence can be acquired within a few hours by means of amplification of each exon and splicing using overlap-PCR. Thus, the new approach developed in this study is simple, time- and cost-effective, and it is not limited to particular gene expression levels of each tissue.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.14 n.3 20112011-05-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300012en |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
cDNA cloning hLIF oral mucous membrane overlap PCR |
| spellingShingle |
cDNA cloning hLIF oral mucous membrane overlap PCR Cui,Yihong Zhu,Guangqin Chen,Qiuju Wang,Yunfei Yang,Mingming Song,Yuxuan Wang,Jiangang Cao,Binyun A preferable approach to clone hLIF cDNA from the genomic DNA |
| description |
Complementary DNA (cDNA) is valuable for investigating protein structure and function in the research of life science, but it is difficult to obtain by traditional reverse transcription. In this study, we employed a novel strategy to clone the human leukemia inhibitory factor (hLIF) gene cDNA from genomic DNA directly isolated from the mucous membrane of mouth. The hLIF sequence can be acquired within a few hours by means of amplification of each exon and splicing using overlap-PCR. Thus, the new approach developed in this study is simple, time- and cost-effective, and it is not limited to particular gene expression levels of each tissue. |
| author |
Cui,Yihong Zhu,Guangqin Chen,Qiuju Wang,Yunfei Yang,Mingming Song,Yuxuan Wang,Jiangang Cao,Binyun |
| author_facet |
Cui,Yihong Zhu,Guangqin Chen,Qiuju Wang,Yunfei Yang,Mingming Song,Yuxuan Wang,Jiangang Cao,Binyun |
| author_sort |
Cui,Yihong |
| title |
A preferable approach to clone hLIF cDNA from the genomic DNA |
| title_short |
A preferable approach to clone hLIF cDNA from the genomic DNA |
| title_full |
A preferable approach to clone hLIF cDNA from the genomic DNA |
| title_fullStr |
A preferable approach to clone hLIF cDNA from the genomic DNA |
| title_full_unstemmed |
A preferable approach to clone hLIF cDNA from the genomic DNA |
| title_sort |
preferable approach to clone hlif cdna from the genomic dna |
| publisher |
Pontificia Universidad Católica de Valparaíso |
| publishDate |
2011 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300012 |
| work_keys_str_mv |
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