A preferable approach to clone hLIF cDNA from the genomic DNA

A preferable approach to clone hLIF cDNA from the genomic DNA

Complementary DNA (cDNA) is valuable for investigating protein structure and function in the research of life science, but it is difficult to obtain by traditional reverse transcription. In this study, we employed a novel strategy to clone the human leukemia inhibitory factor (hLIF) gene cDNA from g...

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Autores principales: Cui,Yihong, Zhu,Guangqin, Chen,Qiuju, Wang,Yunfei, Yang,Mingming, Song,Yuxuan, Wang,Jiangang, Cao,Binyun
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso 2011
Materias:
cDNA cloning
hLIF
oral mucous membrane
overlap PCR
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300012
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