Convenient genotyping of nine bovine K-casein variants

K-casein gene polymorphisms are of major importance in the dairy industry due to their association with different quality and productive traits (i.e., milk protein). Several methods for genotyping this gene have been proposed; however, none are focused on the simultaneous discrimination of nine K-ca...

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Autores principales:	Pacheco Contreras,Víctor I, Lourenco Jaramillo,Diana L, Parra Bracamonte,Gaspar M, Martínez González,Juan C, Sifuentes Rincón,Ana M
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2011
Materias:	milk proteins milk quality protein genes
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000400012
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spelling	oai:scielo:S0717-345820110004000122012-04-17Convenient genotyping of nine bovine K-casein variantsPacheco Contreras,Víctor ILourenco Jaramillo,Diana LParra Bracamonte,Gaspar MMartínez González,Juan CSifuentes Rincón,Ana M milk proteins milk quality protein genes K-casein gene polymorphisms are of major importance in the dairy industry due to their association with different quality and productive traits (i.e., milk protein). Several methods for genotyping this gene have been proposed; however, none are focused on the simultaneous discrimination of nine K-casein variants. A strategy based on PCR-RFLP was designed to characterize nine K-casein variants (A, B, C, E, F², G, H, I and J) and used to genotype three cattle populations: Gyrholando (3/8 Gyr x 5/8 Holstein), Charolais and Carora. The B variant was the most frequent in the Charolais and Carora breeds, with allelic frequencies of 0.60 and 0.59, respectively. In the Gyrholando breed, four variants were found, with the A variant being the most frequent and E and H the least. The genotyping strategy was effective in detection and differentiation of K-casein variants, and it is proposed for use in laboratories with minimal molecular biology equipment for genotyping and evaluation of the phenotypic effects of nine K-casein variants on milk production and quality.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.14 n.4 20112011-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000400012en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	milk proteins milk quality protein genes
spellingShingle	milk proteins milk quality protein genes Pacheco Contreras,Víctor I Lourenco Jaramillo,Diana L Parra Bracamonte,Gaspar M Martínez González,Juan C Sifuentes Rincón,Ana M Convenient genotyping of nine bovine K-casein variants
description	K-casein gene polymorphisms are of major importance in the dairy industry due to their association with different quality and productive traits (i.e., milk protein). Several methods for genotyping this gene have been proposed; however, none are focused on the simultaneous discrimination of nine K-casein variants. A strategy based on PCR-RFLP was designed to characterize nine K-casein variants (A, B, C, E, F², G, H, I and J) and used to genotype three cattle populations: Gyrholando (3/8 Gyr x 5/8 Holstein), Charolais and Carora. The B variant was the most frequent in the Charolais and Carora breeds, with allelic frequencies of 0.60 and 0.59, respectively. In the Gyrholando breed, four variants were found, with the A variant being the most frequent and E and H the least. The genotyping strategy was effective in detection and differentiation of K-casein variants, and it is proposed for use in laboratories with minimal molecular biology equipment for genotyping and evaluation of the phenotypic effects of nine K-casein variants on milk production and quality.
author	Pacheco Contreras,Víctor I Lourenco Jaramillo,Diana L Parra Bracamonte,Gaspar M Martínez González,Juan C Sifuentes Rincón,Ana M
author_facet	Pacheco Contreras,Víctor I Lourenco Jaramillo,Diana L Parra Bracamonte,Gaspar M Martínez González,Juan C Sifuentes Rincón,Ana M
author_sort	Pacheco Contreras,Víctor I
title	Convenient genotyping of nine bovine K-casein variants
title_short	Convenient genotyping of nine bovine K-casein variants
title_full	Convenient genotyping of nine bovine K-casein variants
title_fullStr	Convenient genotyping of nine bovine K-casein variants
title_full_unstemmed	Convenient genotyping of nine bovine K-casein variants
title_sort	convenient genotyping of nine bovine k-casein variants
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000400012
work_keys_str_mv	AT pachecocontrerasvictori convenientgenotypingofninebovinekcaseinvariants AT lourencojaramillodianal convenientgenotypingofninebovinekcaseinvariants AT parrabracamontegasparm convenientgenotypingofninebovinekcaseinvariants AT martinezgonzalezjuanc convenientgenotypingofninebovinekcaseinvariants AT sifuentesrinconanam convenientgenotypingofninebovinekcaseinvariants
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Convenient genotyping of nine bovine K-casein variants

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