Antimicrobial effects of plant defence peptides expressed by bovine endothelial cells on intracellular pathogens

Background: The actions of plant antimicrobial peptides (PAP) against intracellular pathogens are poorly known. It has been reported that wheat puroindolines show antibacterial activity against Staphylococcus epidermidis endocyted by macrophages. In this work, we evaluated the intracellular antimicr...

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Autores principales:	Loeza-Ángeles,Heber, López-Meza,Joel E, Ochoa-Zarzosa,Alejandra
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2011
Materias:	antimicrobial peptides Arabidopsis thaliana thionin Capsicum chinense gamma-thionin Candida albicans intracellular pathogens Staphylococcus aureus
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000500001
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spelling	oai:scielo:S0717-345820110005000012012-04-17Antimicrobial effects of plant defence peptides expressed by bovine endothelial cells on intracellular pathogensLoeza-Ángeles,HeberLópez-Meza,Joel EOchoa-Zarzosa,Alejandra antimicrobial peptides Arabidopsis thaliana thionin Capsicum chinense gamma-thionin Candida albicans intracellular pathogens Staphylococcus aureus Background: The actions of plant antimicrobial peptides (PAP) against intracellular pathogens are poorly known. It has been reported that wheat puroindolines show antibacterial activity against Staphylococcus epidermidis endocyted by macrophages. In this work, we evaluated the intracellular antimicrobial activity of PAP gamma-thionin and thionin Thi2.1 produced by bovine endothelial cells against intracellular Staphylococcus aureus and Candida albicans. We used three host-pathogen models: 1) bovine mammary epithelial cells (BMEC)-S. aureus, 2) bovine endothelial cells (BEC)-S. aureus and 3) BEC-C. albicans, and evaluated the effect of conditioned media from BEC producers of PAP (gamma-thionin and thionin Thi2.1). Results: In the first model, conditioned medium (CM) containing Thi2.1 completely inhibited S. aureus intracellular after 24 hrs treatment. In the second model, CM from BEC containing gamma-thionin has a better effect killing intracellular S. aureus for 12-24 hrs incubations than CM from endothelial cells producers of Thi2.1; this was related with an increase of nitric oxide production (~2 times) in BEC infected and treated for 12 hrs with CM containing gamma-thionin, which negatively correlates with bacterial viability. In the third model, CM containing Thi2.1 showed a more potent intracellular fungicidal activity (~85% of inhibition) at 24 hrs treatment than CM containing gamma-thionin (~35% of inhibition). Conclusions: This work shows new effects of PAP to control intracellular bacterial or fungal infections.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.14 n.5 20112011-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000500001en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	antimicrobial peptides Arabidopsis thaliana thionin Capsicum chinense gamma-thionin Candida albicans intracellular pathogens Staphylococcus aureus
spellingShingle	antimicrobial peptides Arabidopsis thaliana thionin Capsicum chinense gamma-thionin Candida albicans intracellular pathogens Staphylococcus aureus Loeza-Ángeles,Heber López-Meza,Joel E Ochoa-Zarzosa,Alejandra Antimicrobial effects of plant defence peptides expressed by bovine endothelial cells on intracellular pathogens
description	Background: The actions of plant antimicrobial peptides (PAP) against intracellular pathogens are poorly known. It has been reported that wheat puroindolines show antibacterial activity against Staphylococcus epidermidis endocyted by macrophages. In this work, we evaluated the intracellular antimicrobial activity of PAP gamma-thionin and thionin Thi2.1 produced by bovine endothelial cells against intracellular Staphylococcus aureus and Candida albicans. We used three host-pathogen models: 1) bovine mammary epithelial cells (BMEC)-S. aureus, 2) bovine endothelial cells (BEC)-S. aureus and 3) BEC-C. albicans, and evaluated the effect of conditioned media from BEC producers of PAP (gamma-thionin and thionin Thi2.1). Results: In the first model, conditioned medium (CM) containing Thi2.1 completely inhibited S. aureus intracellular after 24 hrs treatment. In the second model, CM from BEC containing gamma-thionin has a better effect killing intracellular S. aureus for 12-24 hrs incubations than CM from endothelial cells producers of Thi2.1; this was related with an increase of nitric oxide production (~2 times) in BEC infected and treated for 12 hrs with CM containing gamma-thionin, which negatively correlates with bacterial viability. In the third model, CM containing Thi2.1 showed a more potent intracellular fungicidal activity (~85% of inhibition) at 24 hrs treatment than CM containing gamma-thionin (~35% of inhibition). Conclusions: This work shows new effects of PAP to control intracellular bacterial or fungal infections.
author	Loeza-Ángeles,Heber López-Meza,Joel E Ochoa-Zarzosa,Alejandra
author_facet	Loeza-Ángeles,Heber López-Meza,Joel E Ochoa-Zarzosa,Alejandra
author_sort	Loeza-Ángeles,Heber
title	Antimicrobial effects of plant defence peptides expressed by bovine endothelial cells on intracellular pathogens
title_short	Antimicrobial effects of plant defence peptides expressed by bovine endothelial cells on intracellular pathogens
title_full	Antimicrobial effects of plant defence peptides expressed by bovine endothelial cells on intracellular pathogens
title_fullStr	Antimicrobial effects of plant defence peptides expressed by bovine endothelial cells on intracellular pathogens
title_full_unstemmed	Antimicrobial effects of plant defence peptides expressed by bovine endothelial cells on intracellular pathogens
title_sort	antimicrobial effects of plant defence peptides expressed by bovine endothelial cells on intracellular pathogens
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000500001
work_keys_str_mv	AT loezaangelesheber antimicrobialeffectsofplantdefencepeptidesexpressedbybovineendothelialcellsonintracellularpathogens AT lopezmezajoele antimicrobialeffectsofplantdefencepeptidesexpressedbybovineendothelialcellsonintracellularpathogens AT ochoazarzosaalejandra antimicrobialeffectsofplantdefencepeptidesexpressedbybovineendothelialcellsonintracellularpathogens
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Antimicrobial effects of plant defence peptides expressed by bovine endothelial cells on intracellular pathogens

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