Effects of substrates and reaction conditions on production of cyclodextrins using cyclodextrin glucanotransferase from newly isolated Bacillus agaradhaerens KSU-A11

The effects of reaction conditions on cyclodextrins (CDs) production by CGTase from newly isolated Bacillus agaradhaerens KSU-A11 is reported. Among six types of starch tested, potato starch gave highest starch conversion into CDs. In addition, CDs yield was about three fold higher when using gelati...

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Autores principales:	Ibrahim,Abdelnasser S.S, El-Tayeb,Mohamed A, Elbadawi,Yahya B, Al-Salamah,Ali A
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2011
Materias:	Bacillus agaradhaerens cyclodextrin glucanotransferase cyclodextrinc enzymatic reaction starch
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000500004
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spelling	oai:scielo:S0717-345820110005000042012-04-17Effects of substrates and reaction conditions on production of cyclodextrins using cyclodextrin glucanotransferase from newly isolated Bacillus agaradhaerens KSU-A11Ibrahim,Abdelnasser S.SEl-Tayeb,Mohamed AElbadawi,Yahya BAl-Salamah,Ali A Bacillus agaradhaerens cyclodextrin glucanotransferase cyclodextrinc enzymatic reaction starch The effects of reaction conditions on cyclodextrins (CDs) production by CGTase from newly isolated Bacillus agaradhaerens KSU-A11 is reported. Among six types of starch tested, potato starch gave highest starch conversion into CDs. In addition, CDs yield was about three fold higher when using gelatinized potato starch in comparison to raw starch. The total CDs production was increased with increasing pH, showing maximum starch conversion at pH 10. Furthermore, the proportion of γ-CD was relatively higher under slightly acidic-neutral conditions than at alkaline pH with a maximum proportion of 35.6% at pH 7 compared to 7.6% at pH 10. Maximum starch conversion into CDs was seen at reaction temperature of 55ºC. Lower reaction temperature led to higher proportion of γ-CD with maximum percentage at 35ºC. Cyclization reaction was significantly promoted in the presence CaCl2 (10 mM), while in the presence of ethyl alcohol there was significant decrease in CD production particularly at high concentration. β-CD was the major product up to 1 hr reaction period with traces of α-CD and no detectable γ-CD. However, as the reaction proceed, γ-CD started to be synthesised and α-CD concentration increased up to 4 hrs, where the CDs ratios were 0.27:0.65:0.07 for α-CD:β-CD:γ-CD, respectively. In addition, optimum CGTase/starch ratio was obtained at 80 U/g starch, showing highest starch conversion into CDs. All the parameters involved have been shown to affect the products yield and/or specificity of B. agaradhaerens KSU-A11 CGTase.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.14 n.5 20112011-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000500004en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Bacillus agaradhaerens cyclodextrin glucanotransferase cyclodextrinc enzymatic reaction starch
spellingShingle	Bacillus agaradhaerens cyclodextrin glucanotransferase cyclodextrinc enzymatic reaction starch Ibrahim,Abdelnasser S.S El-Tayeb,Mohamed A Elbadawi,Yahya B Al-Salamah,Ali A Effects of substrates and reaction conditions on production of cyclodextrins using cyclodextrin glucanotransferase from newly isolated Bacillus agaradhaerens KSU-A11
description	The effects of reaction conditions on cyclodextrins (CDs) production by CGTase from newly isolated Bacillus agaradhaerens KSU-A11 is reported. Among six types of starch tested, potato starch gave highest starch conversion into CDs. In addition, CDs yield was about three fold higher when using gelatinized potato starch in comparison to raw starch. The total CDs production was increased with increasing pH, showing maximum starch conversion at pH 10. Furthermore, the proportion of γ-CD was relatively higher under slightly acidic-neutral conditions than at alkaline pH with a maximum proportion of 35.6% at pH 7 compared to 7.6% at pH 10. Maximum starch conversion into CDs was seen at reaction temperature of 55ºC. Lower reaction temperature led to higher proportion of γ-CD with maximum percentage at 35ºC. Cyclization reaction was significantly promoted in the presence CaCl2 (10 mM), while in the presence of ethyl alcohol there was significant decrease in CD production particularly at high concentration. β-CD was the major product up to 1 hr reaction period with traces of α-CD and no detectable γ-CD. However, as the reaction proceed, γ-CD started to be synthesised and α-CD concentration increased up to 4 hrs, where the CDs ratios were 0.27:0.65:0.07 for α-CD:β-CD:γ-CD, respectively. In addition, optimum CGTase/starch ratio was obtained at 80 U/g starch, showing highest starch conversion into CDs. All the parameters involved have been shown to affect the products yield and/or specificity of B. agaradhaerens KSU-A11 CGTase.
author	Ibrahim,Abdelnasser S.S El-Tayeb,Mohamed A Elbadawi,Yahya B Al-Salamah,Ali A
author_facet	Ibrahim,Abdelnasser S.S El-Tayeb,Mohamed A Elbadawi,Yahya B Al-Salamah,Ali A
author_sort	Ibrahim,Abdelnasser S.S
title	Effects of substrates and reaction conditions on production of cyclodextrins using cyclodextrin glucanotransferase from newly isolated Bacillus agaradhaerens KSU-A11
title_short	Effects of substrates and reaction conditions on production of cyclodextrins using cyclodextrin glucanotransferase from newly isolated Bacillus agaradhaerens KSU-A11
title_full	Effects of substrates and reaction conditions on production of cyclodextrins using cyclodextrin glucanotransferase from newly isolated Bacillus agaradhaerens KSU-A11
title_fullStr	Effects of substrates and reaction conditions on production of cyclodextrins using cyclodextrin glucanotransferase from newly isolated Bacillus agaradhaerens KSU-A11
title_full_unstemmed	Effects of substrates and reaction conditions on production of cyclodextrins using cyclodextrin glucanotransferase from newly isolated Bacillus agaradhaerens KSU-A11
title_sort	effects of substrates and reaction conditions on production of cyclodextrins using cyclodextrin glucanotransferase from newly isolated bacillus agaradhaerens ksu-a11
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000500004
work_keys_str_mv	AT ibrahimabdelnasserss effectsofsubstratesandreactionconditionsonproductionofcyclodextrinsusingcyclodextringlucanotransferasefromnewlyisolatedbacillusagaradhaerensksua11 AT eltayebmohameda effectsofsubstratesandreactionconditionsonproductionofcyclodextrinsusingcyclodextringlucanotransferasefromnewlyisolatedbacillusagaradhaerensksua11 AT elbadawiyahyab effectsofsubstratesandreactionconditionsonproductionofcyclodextrinsusingcyclodextringlucanotransferasefromnewlyisolatedbacillusagaradhaerensksua11 AT alsalamahalia effectsofsubstratesandreactionconditionsonproductionofcyclodextrinsusingcyclodextringlucanotransferasefromnewlyisolatedbacillusagaradhaerensksua11
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Effects of substrates and reaction conditions on production of cyclodextrins using cyclodextrin glucanotransferase from newly isolated Bacillus agaradhaerens KSU-A11

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